LOCUS       V041067                 9814 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041067
VERSION     V041067
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..3840
                     /label="Ugp2(NM_139297)"
                     /note="Ugp2(NM_139297)"
                     /gene="Ugp2"
     CDS             3847..3912
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        3956..4167
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4180..4448
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4477..4486
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4507..5169
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5239..5292
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5293..5892
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5893..6481
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6555..6788
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6860..6981
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7021..7156
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7189..7205)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7213..7229
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7237..7267)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7282..7303
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7591..8179)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8350..9210)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9211..9315)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9789..9805
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt cgagatttgt acaagatctt
     2341 agcaaagcta tgtctcaaga tggtgcttct cagttccaag aggtcattct ccaagaacta
     2401 gaattatctg tgaagaaaga attagaaaaa atacttacca cagcagcctc acatgagttt
     2461 gagcacacta aaaaagatct tgatggattt cggaagctat ttcatagatt tttgcaagaa
     2521 aagggaccgt ctgtggactg gggtaaaatc cagagacctc cagaagattc gattcaaccc
     2581 tatgaaaaga taaaagccag aggcttgcct gataacatat cttctgtgtt gaacaagttg
     2641 gtggtagtga aactcaatgg tggtttggga accagcatgg gctgcaaagg ccctaaaagt
     2701 ctgattggtg taagaaatga gaacaccttt ttggatctaa ctgttcagca aattgaacat
     2761 ttgaacaaaa cctataatac agatgttcct ctcgttttaa tgaattcttt taacacggat
     2821 gaagatacca aaaaaatact ccagaagtac aatcactgtc gtgtgaaaat ctacaccttc
     2881 aatcaaagca ggtacccgag gatcaataag gaatctctac ttcctatagc aaaggatgtg
     2941 tcttattcag gggaaaatac tgaagcttgg taccctccag ggcatggaga tatctatgct
     3001 agtttctaca attctggctt gctcgatacc tttatagaag aaggcaaaga gtatattttt
     3061 gtgtctaaca tagataatct gggtgccaca gtggatcttt atattcttaa tcacctgatg
     3121 aacccaccca atgggaagcg ctgtgagttt gtcatggaag tcacaaacaa aacacgagca
     3181 gatgttaagg gtgggacact cactcagtat gaaggcaaac tgagactggt ggaaatcgct
     3241 caagtgccca aagcacacgt tgatgagttc aagtctgtgt caaaatttaa aatatttaac
     3301 acaaacaacc tatggatttc tcttggagca gttaaaagac ttcaggaaca gaatgccatt
     3361 gacatggaaa tcattgtgaa tccaaagaca ttggatggag gcctgaatgt aattcagtta
     3421 gaaactgcag ttggagctgc aattaaaagt ttcgagaatt ctttaggtat taatgttcca
     3481 aggagtcgtt ttctgcctgt gaaaaccaca tcagatctct tgcttgtgat gtcaaacctc
     3541 tatagcctta acgcaggatc tctgaccatg agtgaaaagc gtgaattccc tacagtgccc
     3601 ttggttaaat taggcagttc ttttaccaag gttcaagatt atctaagaag gtttgaaagt
     3661 atacccgata tgctggaact ggaccacctc actgtttcag gagatgtgac atttggaaaa
     3721 aatgtttcac taaagggaac agttattatt attgcaaatc atggtgacag aattgacatc
     3781 cccccaggag ccgtgttaga gaacaagatt gtatctggaa accttcggat cctggatcac
     3841 ggatccgact acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat
     3901 gacgatgaca agtagcggcc gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca
     3961 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt
     4021 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     4081 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     4141 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca
     4201 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct
     4261 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg
     4321 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg
     4381 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt
     4441 tacagatcca agctgtgacc ggcgcctacg ctagacgcca ccatggagag cgacgagagc
     4501 ggcctgcccg ccatggagat cgagtgccgc atcaccggca ccctgaacgg cgtggagttc
     4561 gagctggtgg gcggcggaga gggcaccccc aagcagggcc gcatgaccaa caagatgaag
     4621 agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat gggctacggc
     4681 ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca cgccatcaac
     4741 aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg
     4801 agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc
     4861 ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa cgccaccgtg
     4921 gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg caccttcagc
     4981 ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt caagagcgcc
     5041 atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg cgtggaggag
     5101 ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa gacccccatc
     5161 gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg tggacggcac cgccggaccc
     5221 ggctccaccg gatctcgcga gggcagagga agtcttctaa catgcggtga cgtggaggag
     5281 aatcccggcc ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     5341 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     5401 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     5461 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     5521 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     5581 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     5641 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     5701 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     5761 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     5821 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     5881 cccggtgcct gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     5941 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     6001 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     6061 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     6121 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     6181 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     6241 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     6301 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     6361 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     6421 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     6481 cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     6541 agaaaagggg ggactggaag ggctaattca ctcccaacga agataagatc tgctttttgc
     6601 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     6661 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     6721 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     6781 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     6841 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     6901 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     6961 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     7021 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     7081 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     7141 ggcttttttg gaggcctaga cttttgcaga gaccaaattc gtaatcatgt catagctgtt
     7201 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     7261 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     7321 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     7381 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     7441 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     7501 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     7561 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     7621 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     7681 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     7741 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     7801 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     7861 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     7921 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     7981 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     8041 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     8101 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     8161 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     8221 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     8281 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     8341 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     8401 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     8461 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     8521 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     8581 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     8641 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     8701 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     8761 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     8821 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     8881 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     8941 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     9001 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     9061 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     9121 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     9181 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     9241 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     9301 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     9361 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
     9421 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     9481 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     9541 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     9601 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt
     9661 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     9721 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
     9781 acgacgttgt aaaacgacgg ccagtgccaa gctg
//
