LOCUS       V025862                10087 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025862
VERSION     V025862
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4113
                     /label="ZC3H12A(NM_001323550)"
                     /note="ZC3H12A(NM_001323550)"
                     /gene="ZC3H12A"
     CDS             4120..4185
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4229..4440
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4453..4721
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4750..4759
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4780..5442
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5512..5565
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5566..6165
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6166..6754
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6828..7061
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7133..7254
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7294..7429
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7462..7478)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7486..7502
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7510..7540)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7555..7576
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7864..8452)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8623..9483)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9484..9588)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10062..10078
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga gtggcccctg tggagagaag
     2341 cctgtcctgg aagccagccc caccatgagt ctgtgggaat ttgaggacag ccacagccgt
     2401 cagggcaccc caaggccggg tcaagagctg gccgctgagg aggcctcggc cctggaactg
     2461 cagatgaagg tggacttctt ccggaagctg ggctattcat ccacggagat ccacagcgtc
     2521 ctgcagaagc tgggcgtcca ggcagacacc aacacggtgc tgggtgagct ggtgaaacac
     2581 gggacagcca ccgagcggga gcgccagacc tcaccggacc cctgccctca gctccctcta
     2641 gtcccgcggg gtggtggcac ccctaaggct cccaacctgg agcctccact cccagaagag
     2701 gaaaaggagg gcagcgacct gagaccagtg gtcatcgatg ggagcaacgt ggccatgagc
     2761 catgggaaca aggaggtctt ctcctgccgg ggcatcctgc tggcagtgaa ctggtttctg
     2821 gagcggggcc acacagacat cacagtgttt gtgccatcct ggaggaagga gcagcctcgg
     2881 cccgacgtgc ccatcacaga ccagcacatc ctgcgggaac tggagaagaa gaagatcctg
     2941 gtgttcacac catcacgacg cgtgggtggc aagcgggtgg tgtgctatga cgacagattc
     3001 attgtgaagc tggcctacga gtctgacggg atcgtggttt ccaacgacac ataccgtgac
     3061 ctccaaggcg agcggcagga gtggaagcgc ttcatcgagg agcggctgct catgtactcc
     3121 ttcgtcaatg acaagtttat gccccctgat gacccactgg gccggcacgg gcccagcctg
     3181 gacaacttcc tgcgtaagaa gccactcact ttggagcaca ggaagcagcc gtgtccctat
     3241 ggaaggaaat gcacctatgg gatcaagtgc cgattcttcc acccagagcg gccaagctgc
     3301 ccccagcgct ctgtggcaga tgagctccgt gccaatgctc tcctctcacc ccccagagcc
     3361 ccaagcaagg acaaaaatgg ccggcggcct tcaccttcat cccagtccag ctctctgcta
     3421 acagagagtg agcagtgcag cctggatggg aagaagctgg gggcccaggc atccccaggg
     3481 tcccgccaag agggtctaac acagacctat gccccatcag gcaggagcct cgcacctagc
     3541 gggggcagtg gcagcagctt tgggcccaca gactggctcc cacagacgct ggactcactc
     3601 ccgtacgtct cccaggattg cctggactcg ggcattggct ccctggagag ccagatgtcg
     3661 gaactttggg gggttcgagg aggaggccct ggtgagccgg gcccaccccg agccccttac
     3721 acgggctaca gtccctatgg atctgagctc ccagccaccg cagccttctc tgcctttggc
     3781 cgggccatgg gtgctggcca cttcagtgtc cctgccgact acccacccgc gccccctgcc
     3841 tttccacctc gagagtactg gtctgaacca tacccactgc ccccacccac atcagtcctt
     3901 caggagcccc cagtgcagag cccaggggct ggcaggagcc cgtggggcag ggcaggcagc
     3961 ctggccaagg agcaggccag cgtgtatact aagctgtgtg gtgtgtttcc cccgcacctg
     4021 gtggaggctg tgatggggcg cttcccacag ctcctggacc cccagcagct ggctgccgag
     4081 atcctctcct acaagtccca gcaccccagt gagggatccg actacaaaga ccatgacggt
     4141 gattataaag atcatgacat cgactacaag gatgacgatg acaagtagcg gccgcgaagg
     4201 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     4261 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac
     4321 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     4381 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     4441 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc
     4501 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg
     4561 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct
     4621 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc
     4681 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct
     4741 acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc
     4801 cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc
     4861 cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc
     4921 ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc
     4981 ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc
     5041 gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc
     5101 cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc
     5161 accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac
     5221 gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc
     5281 gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg
     5341 ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc
     5401 gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg
     5461 tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga
     5521 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag
     5581 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     5641 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     5701 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     5761 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     5821 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     5881 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     5941 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     6001 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     6061 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     6121 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat
     6181 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     6241 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     6301 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     6361 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     6421 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     6481 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     6541 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     6601 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     6661 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     6721 acgagtcgga tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta
     6781 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     6841 tcactcccaa cgaagataag atctgctttt tgcttgtact gggtctctct ggttagacca
     6901 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     6961 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     7021 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     7081 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     7141 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     7201 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     7261 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     7321 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     7381 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc
     7441 agagaccaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac
     7501 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     7561 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     7621 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     7681 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     7741 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     7801 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     7861 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     7921 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     7981 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     8041 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     8101 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8161 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8221 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     8281 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     8341 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     8401 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     8461 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     8521 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     8581 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     8641 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     8701 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     8761 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     8821 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     8881 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     8941 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     9001 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     9061 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     9121 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     9181 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     9241 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     9301 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     9361 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     9421 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     9481 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     9541 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     9601 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     9661 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     9721 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     9781 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     9841 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     9901 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
     9961 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
    10021 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc
    10081 caagctg
//
