LOCUS       V044502                10435 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044502
VERSION     V044502
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4461
                     /label="ZDHHC5(NM_015457)"
                     /note="ZDHHC5(NM_015457)"
                     /gene="ZDHHC5"
     CDS             4468..4533
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4577..4788
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4801..5069
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5098..5107
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5128..5790
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5860..5913
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5914..6513
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6514..7102
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7176..7409
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7481..7602
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7642..7777
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7810..7826)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7834..7850
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7858..7888)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7903..7924
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8212..8800)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8971..9831)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9832..9936)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10410..10426
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc ccgcagagtc tggaaagaga
     2341 ttcaaaccca gcaagtatgt cccggtctct gcagccgcca tcttcctagt gggagctacg
     2401 acactcttct ttgcctttac gtgtccagga ctaagcctgt atgtgtcacc tgcagtgccc
     2461 atctacaatg caattatgtt tctctttgtg ttggccaact tcagcatggc caccttcatg
     2521 gacccaggga ttttccctcg agctgaggag gatgaggaca aggaagatga tttccgagct
     2581 cccctttaca aaacagtgga gataaagggc atccaggtgc gcatgaaatg gtgtgccacc
     2641 tgccgctttt accgtccccc tcgatgttcc cactgcagtg tctgtgacaa ctgtgtggag
     2701 gaatttgatc atcactgccc ctgggtgaat aactgtattg gtcgccggaa ctaccgttat
     2761 tttttccttt tcctcctttc cctgacagcc cacattatgg gtgtgtttgg ctttggcctc
     2821 ctttatgtcc tctaccacat agaggaactc tcaggggtcc gcacggctgt cacaatggca
     2881 gtaatgtgtg tggctggctt attcttcatc cctgtagctg gcctcacggg atttcacgtg
     2941 gttctggtgg ccaggggacg cacaaccaat gaacaggtta cgggtaaatt ccggggaggt
     3001 gtgaacccct tcaccaatgg ctgctgtaac aatgtcagcc gtgttctctg cagttctcca
     3061 gcacccaggt atttggggag accaaagaaa gagaagacaa ttgtaatcag acctcccttc
     3121 cttcgaccag aagtttcaga tgggcagata actgtgaaga tcatggataa tggcatccag
     3181 ggagagctga ggagaacaaa gtctaaggga agcctggaga taacagagag ccagtctgca
     3241 gatgctgaac ctccacctcc tcctaagcca gacctgagcc gttacacagg gttgcgaaca
     3301 cacctcggct tggctactaa tgaggatagt agcttattgg ccaaggacag ccccccgaca
     3361 cctaccatgt acaagtatcg gccgggttac agtagcagca gtacgtcagc tgccatgccg
     3421 cattcctcca gcgccaagtt gagtcgtggg gacagcttga aggagccaac ctcaattgca
     3481 gagagcagcc gtcaccccag ctaccgctca gagcccagct tggaaccaga gagcttccgt
     3541 tctcctacct ttggcaaaag ttttcacttc gatccactat ccagtggctc acgctcctcc
     3601 agcctcaagt cagcccaggg cacaggcttt gagctgggcc agttgcaatc cattcgttca
     3661 gagggcacca cctccacctc ctataagagc ctggccaacc agacacgcaa tggaagccta
     3721 tcttatgaca gcttgctcac accttcagac agccctgatt ttgagtcagt gcaggcaggg
     3781 cctgagccag acccaccttt aggctatacc tctcccttcc tgtcagccag gctggcccag
     3841 caacgggaag ctgagaggca cccacgtttg gtgccaactg gcccaacaca ccgagagccc
     3901 tcaccagtcc gttacgacaa tctgtcgcgc cacattgtgg cctctctcca ggaacgagag
     3961 aagttgctgc gccagtcacc cccactcccg ggccgtgagg aagaaccagg cttgggggac
     4021 tcaggcattc agtcaacacc aggctcgggc catgcccctc gtactagttc ctcctcagat
     4081 gattcaaaga gatcaccttt gggcaagact ccactgggac gcccagctgt cccccgtttt
     4141 ggcaagccag atgggctaag gggccgggga gtagggtccc ctgaaccagg cccaacagcc
     4201 ccatacctgg gccgatcgat gtcttacagc agccaaaaag cccaacctgg tgtctctgag
     4261 acagaagaag tggccttgca gccattactg acacccaaag atgaagtaca gctgaagacc
     4321 acctacagca aatccaacgg gcagcccaag agcttaggct cagcctcccc tggcccaggc
     4381 cagccacctc tcagtagccc cacgagggga ggagtcaaga aggtgtcagg ggttggtggt
     4441 accacctatg agatttcggt gggatccgac tacaaagacc atgacggtga ttataaagat
     4501 catgacatcg actacaagga tgacgatgac aagtagcggc cgcgaaggat ctgcgatcgc
     4561 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga
     4621 ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat
     4681 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta
     4741 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga
     4801 ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg
     4861 ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag
     4921 gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct
     4981 agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt
     5041 tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gctagacgcc
     5101 accatggaga gcgacgagag cggcctgccc gccatggaga tcgagtgccg catcaccggc
     5161 accctgaacg gcgtggagtt cgagctggtg ggcggcggag agggcacccc caagcagggc
     5221 cgcatgacca acaagatgaa gagcaccaaa ggcgccctga ccttcagccc ctacctgctg
     5281 agccacgtga tgggctacgg cttctaccac ttcggcacct accccagcgg ctacgagaac
     5341 cccttcctgc acgccatcaa caacggcggc tacaccaaca cccgcatcga gaagtacgag
     5401 gacggcggcg tgctgcacgt gagcttcagc taccgctacg aggccggccg cgtgatcggc
     5461 gacttcaagg tggtgggcac cggcttcccc gaggacagcg tgatcttcac cgacaagatc
     5521 atccgcagca acgccaccgt ggagcacctg caccccatgg gcgataacgt gctggtgggc
     5581 agcttcgccc gcaccttcag cctgcgcgac ggcggctact acagcttcgt ggtggacagc
     5641 cacatgcact tcaagagcgc catccacccc agcatcctgc agaacggggg ccccatgttc
     5701 gccttccgcc gcgtggagga gctgcacagc aacaccgagc tgggcatcgt ggagtaccag
     5761 cacgccttca agacccccat cgccttcgcc agatcccgcg ctcagtcgtc caattctgcc
     5821 gtggacggca ccgccggacc cggctccacc ggatctcgcg agggcagagg aagtcttcta
     5881 acatgcggtg acgtggagga gaatcccggc cctatgaccg agtacaagcc cacggtgcgc
     5941 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     6001 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     6061 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     6121 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     6181 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     6241 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     6301 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     6361 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     6421 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     6481 acctggtgca tgacccgcaa gcccggtgcc tgaaatcaac ctctggatta caaaatttgt
     6541 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     6601 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     6661 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     6721 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     6781 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     6841 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     6901 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     6961 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     7021 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     7081 tccctttggg ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt
     7141 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     7201 aagataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     7261 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7321 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     7381 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     7441 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     7501 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     7561 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     7621 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     7681 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     7741 ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag agaccaaatt
     7801 cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     7861 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     7921 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     7981 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     8041 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     8101 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     8161 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     8221 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     8281 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     8341 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     8401 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     8461 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     8521 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     8581 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     8641 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     8701 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     8761 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     8821 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     8881 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     8941 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     9001 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     9061 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     9121 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     9181 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     9241 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     9301 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     9361 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     9421 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     9481 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     9541 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     9601 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     9661 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     9721 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     9781 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     9841 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     9901 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     9961 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
    10021 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
    10081 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
    10141 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
    10201 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
    10261 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
    10321 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
    10381 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctg
//
