LOCUS       V034471                11524 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034471
VERSION     V034471
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..3018
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             3055..6378
                     /label="ZEB1(NM_001128128)"
                     /note="ZEB1(NM_001128128)"
                     /gene="ZEB1"
     CDS             6385..6450
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6494..6705
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6718..6986
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             7013..7612
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7619..8207
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8281..8514
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8586..8707
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8726..8861
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8899..8915)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8923..8939
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8947..8977)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8992..9013
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9301..9889)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10060..10920)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10921..11025)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11499..11515
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagattcta
     2281 gagctagcga attcagccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     2341 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     2401 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     2461 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     2521 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     2581 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     2641 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     2701 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     2761 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     2821 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     2881 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     2941 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     3001 atggacgagc tgtacaaggg aggaggggga agcggtggag gcggttctct cgagatgaaa
     3061 gttacaaatt ataatactgt ggtagaaaca aattcagatt cagatgatga agacaaactg
     3121 catattgtgg aagaagaaag tgttacagat gcagctgact gtgaaggtgt accagaggat
     3181 gacctgccaa cagaccagac agtgttacca gggaggagca gtgaaagaga agggaatgct
     3241 aagaactgct gggaggatga cacaggaaag gaagggcaag aaatcctggg gcctgaagct
     3301 caggcagatg aagcaggatg tacagtaaaa gatgatgaat gcgagtcaga tgcagaaaat
     3361 gagcaaaacc atgatcctaa tgttgaagag tttctacaac aacaagacac tgctgtcatt
     3421 tttcctgagg cacctgaaga ggaccagagg cagggcacac cagaagccag tggtcatgat
     3481 gaaaatggaa caccagatgc attttcacaa ttactcacct gtccatattg tgatagaggc
     3541 tataaacgct ttacctctct gaaagaacac attaaatatc gtcatgaaaa gaatgaagat
     3601 aactttagtt gctccctgtg cagttacacc tttgcataca gaacccaact tgaacgtcac
     3661 atgacatcac ataaatcagg aagagatcaa agacatgtga cgcagtctgg gtgtaatcgt
     3721 aaattcaaat gcactgagtg tggaaaagct ttcaaataca aacatcacct aaaagagcac
     3781 ttaagaattc acagtggaga gaagccatat gaatgcccaa actgcaagaa acgcttttcc
     3841 cattctggct cctatagctc acacataagc agtaagaaat gtatcagctt gatacctgtg
     3901 aatgggcgac caagaacagg actcaagaca tctcagtgtt cttcaccgtc tctttcagca
     3961 tcaccaggca gtcccacacg accacagata cggcaaaaga tagagaataa accccttcaa
     4021 gaacaacttt ctgttaacca aattaaaact gaacctgtgg attatgaatt caaacccata
     4081 gtggttgctt caggaatcaa ctgttcaacc cctttacaaa atggggtttt cactggtggt
     4141 ggcccattac aggcaaccag ttctcctcag ggcatggtgc aagctgttgt tctgccaaca
     4201 gttggtttgg tgtctcccat aagtatcaat ttaagtgata ttcagaatgt acttaaagtg
     4261 gcggtagatg gtaatgtaat aaggcaagtg ttggagaata atcaagccaa tcttgcatcc
     4321 aaagaacaag aaacaatcaa tgcttcaccc atacaacaag gtggccattc tgttatttca
     4381 gccatcagtc ttcctttggt tgatcaagat ggaacaacca aaattatcat caactacagt
     4441 cttgagcagc ctagccaact tcaagttgtt cctcaaaatt taaaaaaaga aaatccagtc
     4501 gctacaaaca gttgtaaaag tgaaaagtta ccagaagatc ttactgttaa gtctgagaag
     4561 gacaaaagct ttgaaggggg ggtgaatgat agcacttgtc ttctgtgtga tgattgtcca
     4621 ggagatatta atgcacttcc agaattaaag cactatgacc taaagcagcc tactcagcct
     4681 cctccactcc ctgcagcaga agctgagaag cctgagtcct ctgtttcatc agctactgga
     4741 gatggcaatt tgtctcctag tcagccacct ttaaagaacc tcttgtctct cctaaaagca
     4801 tattatgctt tgaatgcaca accaagtgca gaagagctct caaaaattgc tgattcagta
     4861 aacctaccac tggatgtagt aaaaaagtgg tttgaaaaga tgcaagctgg acagatttca
     4921 gtgcagtctt ctgaaccatc ttctcctgaa ccaggcaaag taaatatccc tgccaagaac
     4981 aatgatcagc ctcaatctgc aaatgcaaat gaaccccagg acagcacagt aaatctacaa
     5041 agtcctttga agatgactaa ctccccagtt ttaccagtgg gatcaaccac caatggttcc
     5101 agaagtagta caccatcccc atcacctcta aacctttcct catccagaaa tacacagggt
     5161 tacttgtaca cagctgaggg tgcacaagaa gagccacaag tagaacctct tgatctttca
     5221 ctaccaaagc aacagggaga attattagaa aggtcaacta tcactagtgt ttaccagaac
     5281 agtgtttatt ctgtccagga agaacccttg aacttgtctt gcgcaaaaaa ggagccacaa
     5341 aaggacagtt gtgttacaga ctcagaacca gttgtaaatg taatcccacc aagtgccaac
     5401 cccataaata tcgctatacc tacagtcact gcccagttac ccacaatcgt ggccattgct
     5461 gaccagaaca gtgttccatg cttaagagcg ctagctgcca ataagcaaac gattctgatt
     5521 ccccaggtgg catacaccta ctcaactacg gtcagccctg cagtccaaga accacccttg
     5581 aaagtgatcc agccaaatgg aaatcaggat gaaagacaag atactagctc agaaggagta
     5641 tcaaatgtag aggatcagaa tgactctgat tctacaccgc ccaaaaagaa aatgcggaag
     5701 acagaaaatg gaatgtatgc ttgtgatttg tgtgacaaga tattccaaaa gagtagttca
     5761 ttattgagac ataaatatga acacacaggt aaaagacctc atgagtgtgg aatctgtaaa
     5821 aaggcattta aacacaaaca tcatttgatt gaacacatgc gattacattc tggagaaaag
     5881 ccctatcaat gtgacaaatg tggaaagcgc ttctcacact ctgggtctta ttctcaacac
     5941 atgaatcatc gctactccta ctgtaagaga gaagcggaag aacgtgacag cacagagcag
     6001 gaagaggcag ggcctgaaat cctctcgaat gagcacgtgg gtgccagggc gtctccctca
     6061 cagggcgact cggacgagag agagagtttg acaagggaag aggatgaaga cagtgaaaaa
     6121 gaggaagagg aggaggataa agagatggaa gaattgcagg aagaaaaaga atgtgaaaaa
     6181 ccacaagggg atgaggaaga ggaggaggag gaggaagaag tggaagaaga agaggtagaa
     6241 gaggcagaga atgagggaga agaagcaaaa actgaaggtc tgatgaagga tgacagggct
     6301 gaaagtcaag caagcagctt aggacaaaaa gtaggcgaga gtagtgagca agtgtctgaa
     6361 gaaaagacaa atgaagccgg atccgactac aaagaccatg acggtgatta taaagatcat
     6421 gacatcgact acaaggatga cgatgacaag tagcggccgc gaaggatctg cgatcgctcc
     6481 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     6541 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     6601 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     6661 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg
     6721 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg
     6781 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta
     6841 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga
     6901 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg
     6961 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agatgaccga
     7021 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct
     7081 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat
     7141 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     7201 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     7261 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     7321 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     7381 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     7441 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     7501 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     7561 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gagtcgacaa
     7621 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     7681 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     7741 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     7801 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     7861 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     7921 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     7981 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     8041 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     8101 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     8161 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gtacctttaa
     8221 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     8281 tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt actgggtctc
     8341 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     8401 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     8461 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     8521 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     8581 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     8641 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     8701 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc
     8761 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     8821 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacgg
     8881 cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     8941 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     9001 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     9061 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     9121 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     9181 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     9241 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     9301 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     9361 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     9421 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     9481 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     9541 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     9601 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     9661 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     9721 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     9781 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     9841 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     9901 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     9961 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    10021 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    10081 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    10141 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    10201 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    10261 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    10321 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    10381 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    10441 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    10501 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    10561 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    10621 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    10681 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    10741 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    10801 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    10861 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    10921 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    10981 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    11041 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
    11101 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
    11161 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
    11221 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
    11281 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
    11341 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
    11401 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
    11461 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa
    11521 gctg
//
