LOCUS       V035856                10565 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035856
VERSION     V035856
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4650
                     /label="ZNF33B(NM_006955)"
                     /note="ZNF33B(NM_006955)"
                     /gene="ZNF33B"
     CDS             4657..4680
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHHHH"
                     /label="8xHis"
     promoter        4724..4935
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4948..5216
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5245..5254
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5275..5937
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6007..6060
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6061..6660
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6661..7248
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7322..7555
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7627..7748
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7767..7902
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7940..7956)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7964..7980
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7988..8018)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8033..8054
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8342..8930)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9101..9961)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9962..10066)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10540..10556
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga acaaggtaga tcagaagttc
     2341 caggggtcag tatcatttaa agatgtgact gtgggcttca cccaggagga gtggcagcac
     2401 ctggacccta gtcagagggc tctgtataga gatgtgatgc tggagaacta cagcaacctt
     2461 gtctcagtgg ggtattgtgc tcacaaacca gaggtgatct tcaggctgga acaaggagaa
     2521 gaaccatgga gactggagga agaattccca agccagagct ttccagaagt ctggacagct
     2581 gatcacctga aagagaggag ccaagaaaat caatctaaac atttgtggga agttgtattc
     2641 atcaataatg aaatgctgac taaggaacaa ggtaatgtaa taggaatacc atttaacatg
     2701 gacgtaagtt cttttccttc cagaaaaatg ttctgtcagt atgactcacg tggaatgagt
     2761 ttcaacactg tttcagaatt ggttatcagt aagataaact atttaggaaa aaagtctgac
     2821 gaatttaatg catgtgggaa attgttactc aatattaagc atgatgaaac tcatactcga
     2881 gagaaaaatg aagttttgaa aaataggaac actctgagtc atcgtgagaa cactttgcag
     2941 catgagaaga ttcaaacttt agaccacaat tttgaataca gtatatgtca ggaaaccctc
     3001 cttgaaaagg cagtattcaa tacacggaag agagagaatg cagaagagaa taactgtgac
     3061 tataatgaat ttgggagaac tttctgtgat agttcatccc tcttgttcca tcagatacct
     3121 ccatcaaagg acagtcacta tgaatttagt gattgtgaga agttcttatg tgtgaagtcc
     3181 accctttcta aacatgatgg ggtacctgtg aaacactatg attgtggtga aagtgggaat
     3241 aatttcagga ggaaattgtg tctgtcacag cttcagaaag gtgataaagg agagaaacac
     3301 tttgaatgta atgaatgtgg gaaagctttc tgggagaagt cacatctcac tcgacatcag
     3361 agggtgcaca caggagagaa acactttcaa tgtaatcaat gtggaaaaac tttctgggag
     3421 aagtcaaacc tcactaaaca tcagagatca cacacagggg agaaaccttt tgaatgcaat
     3481 gaatgtggga aagcctttag ccataagtca gccctcacat tacaccagag aacacataca
     3541 ggggagaaac cctatcagtg taatgcatgt gggaaaactt tttaccagaa atctgacctc
     3601 actaaacatc agagaacaca cacagggcag aaaccctatg aatgttatga atgtggaaaa
     3661 tccttctgta tgaattcaca ccttacagta caccagagaa ctcacacagg tgagaaacct
     3721 tttgaatgtc ttgagtgtgg gaaatccttt tgtcaaaagt cacatcttac acagcatcag
     3781 agaactcaca taggagataa accttatgaa tgtaatgcat gtgggaaaac tttctaccac
     3841 aagtcagtac tcaccaggca tcagataatt catacagggt tgaaacctta tgaatgttat
     3901 gaatgtggga aaaccttctg cttgaagtca gacctcacaa tacatcagag aacgcacaca
     3961 ggggagaaac cctttgcatg tcctgaatgt gggaaattct ttagccataa gtcaaccctc
     4021 tctcaacatt atagaacaca cacgggggag aaaccctatg aatgtcatga atgtggaaaa
     4081 atcttttaca ataaatcata cctaacaaaa cataatagaa cacatacagg ggagaaaccc
     4141 tatgaatgta atgaatgtgg aaaaaccttc tgccagaagt cacaactcac tcagcatcag
     4201 agaattcaca taggggagaa accctatgaa tgtaatgagt gtggaaaagc tttctgccat
     4261 aagtcagctc taattgtaca tcagagaacc catacacaag aaaagcctta taaatgtaac
     4321 gaatgtggaa aatctttctg tgtgaagtca ggacttattt tacatgagag aaagcacacg
     4381 ggggagaaac cctatgaatg caatgaatgt gggaaatcct tcagtcacaa atcatcactc
     4441 acagtacatc acagggctca cacaggagag aaatcttgtc agtgtaatga atgtggaaaa
     4501 atcttttacc gtaaatcaga ccttgctaaa catcagagat cacatacagg ggaaaagccc
     4561 tatgaatgta acacatgcag gaaaaccttc tctcaaaagt caaatctcat tgtacatcag
     4621 agaacacaca taggagaaaa accttatgaa ggatcccacc atcaccatca ccatcaccat
     4681 tagcggccgc gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg
     4741 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg
     4801 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     4861 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     4921 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc
     4981 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt
     5041 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt
     5101 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc
     5161 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag
     5221 ctgtgaccgg cgcctacgct agacgccacc atggagagcg acgagagcgg cctgcccgcc
     5281 atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga gctggtgggc
     5341 ggcggagagg gcacccccaa gcagggccgc atgaccaaca agatgaagag caccaaaggc
     5401 gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt ctaccacttc
     5461 ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa cggcggctac
     5521 accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac
     5581 cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag
     5641 gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac
     5701 cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc
     5761 ggctactaca gcttcgtggt ggacagccac atgcacttca agagcgccat ccaccccagc
     5821 atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagct gcacagcaac
     5881 accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc cttcgccaga
     5941 tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg ctccaccgga
     6001 tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct
     6061 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     6121 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     6181 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     6241 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     6301 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     6361 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     6421 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     6481 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     6541 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     6601 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     6661 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     6721 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     6781 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     6841 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     6901 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     6961 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     7021 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     7081 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     7141 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc
     7201 cgccttcgcc ctcagacgag tcggatctcc ctttggccgc ctccccgcct ggtaccttta
     7261 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     7321 ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct
     7381 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     7441 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     7501 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     7561 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     7621 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     7681 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     7741 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt
     7801 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     7861 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg
     7921 gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     7981 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     8041 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     8101 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     8161 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     8221 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     8281 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     8341 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     8401 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     8461 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     8521 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     8581 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     8641 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     8701 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     8761 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     8821 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     8881 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     8941 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     9001 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     9061 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     9121 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     9181 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     9241 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     9301 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     9361 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     9421 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     9481 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     9541 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     9601 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     9661 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     9721 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     9781 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     9841 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     9901 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     9961 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    10021 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    10081 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
    10141 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
    10201 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
    10261 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
    10321 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
    10381 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat
    10441 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
    10501 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca
    10561 agctg
//
