LOCUS V035856 10565 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V035856 VERSION V035856 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1921 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2011..2214 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2317..4650 /label="ZNF33B(NM_006955)" /note="ZNF33B(NM_006955)" /gene="ZNF33B" CDS 4657..4680 /codon_start=1 /product="8xHis affinity tag" /transl_table=1 /translation="HHHHHHHH" /label="8xHis" promoter 4724..4935 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4948..5216 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" regulatory 5245..5254 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 5275..5937 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /transl_table=1 /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" /label="CopGFP" CDS 6007..6060 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 6061..6660 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 6661..7248 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 7322..7555 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 7627..7748 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 7767..7902 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(7940..7956) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7964..7980 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7988..8018) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 8033..8054 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8342..8930) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(9101..9961) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9962..10066) /gene="bla" /label="AmpR promoter" primer_bind 10540..10556 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga acaaggtaga tcagaagttc 2341 caggggtcag tatcatttaa agatgtgact gtgggcttca cccaggagga gtggcagcac 2401 ctggacccta gtcagagggc tctgtataga gatgtgatgc tggagaacta cagcaacctt 2461 gtctcagtgg ggtattgtgc tcacaaacca gaggtgatct tcaggctgga acaaggagaa 2521 gaaccatgga gactggagga agaattccca agccagagct ttccagaagt ctggacagct 2581 gatcacctga aagagaggag ccaagaaaat caatctaaac atttgtggga agttgtattc 2641 atcaataatg aaatgctgac taaggaacaa ggtaatgtaa taggaatacc atttaacatg 2701 gacgtaagtt cttttccttc cagaaaaatg ttctgtcagt atgactcacg tggaatgagt 2761 ttcaacactg tttcagaatt ggttatcagt aagataaact atttaggaaa aaagtctgac 2821 gaatttaatg catgtgggaa attgttactc aatattaagc atgatgaaac tcatactcga 2881 gagaaaaatg aagttttgaa aaataggaac actctgagtc atcgtgagaa cactttgcag 2941 catgagaaga ttcaaacttt agaccacaat tttgaataca gtatatgtca ggaaaccctc 3001 cttgaaaagg cagtattcaa tacacggaag agagagaatg cagaagagaa taactgtgac 3061 tataatgaat ttgggagaac tttctgtgat agttcatccc tcttgttcca tcagatacct 3121 ccatcaaagg acagtcacta tgaatttagt gattgtgaga agttcttatg tgtgaagtcc 3181 accctttcta aacatgatgg ggtacctgtg aaacactatg attgtggtga aagtgggaat 3241 aatttcagga ggaaattgtg tctgtcacag cttcagaaag gtgataaagg agagaaacac 3301 tttgaatgta atgaatgtgg gaaagctttc tgggagaagt cacatctcac tcgacatcag 3361 agggtgcaca caggagagaa acactttcaa tgtaatcaat gtggaaaaac tttctgggag 3421 aagtcaaacc tcactaaaca tcagagatca cacacagggg agaaaccttt tgaatgcaat 3481 gaatgtggga aagcctttag ccataagtca gccctcacat tacaccagag aacacataca 3541 ggggagaaac cctatcagtg taatgcatgt gggaaaactt tttaccagaa atctgacctc 3601 actaaacatc agagaacaca cacagggcag aaaccctatg aatgttatga atgtggaaaa 3661 tccttctgta tgaattcaca ccttacagta caccagagaa ctcacacagg tgagaaacct 3721 tttgaatgtc ttgagtgtgg gaaatccttt tgtcaaaagt cacatcttac acagcatcag 3781 agaactcaca taggagataa accttatgaa tgtaatgcat gtgggaaaac tttctaccac 3841 aagtcagtac tcaccaggca tcagataatt catacagggt tgaaacctta tgaatgttat 3901 gaatgtggga aaaccttctg cttgaagtca gacctcacaa tacatcagag aacgcacaca 3961 ggggagaaac cctttgcatg tcctgaatgt gggaaattct ttagccataa gtcaaccctc 4021 tctcaacatt atagaacaca cacgggggag aaaccctatg aatgtcatga atgtggaaaa 4081 atcttttaca ataaatcata cctaacaaaa cataatagaa cacatacagg ggagaaaccc 4141 tatgaatgta atgaatgtgg aaaaaccttc tgccagaagt cacaactcac tcagcatcag 4201 agaattcaca taggggagaa accctatgaa tgtaatgagt gtggaaaagc tttctgccat 4261 aagtcagctc taattgtaca tcagagaacc catacacaag aaaagcctta taaatgtaac 4321 gaatgtggaa aatctttctg tgtgaagtca ggacttattt tacatgagag aaagcacacg 4381 ggggagaaac cctatgaatg caatgaatgt gggaaatcct tcagtcacaa atcatcactc 4441 acagtacatc acagggctca cacaggagag aaatcttgtc agtgtaatga atgtggaaaa 4501 atcttttacc gtaaatcaga ccttgctaaa catcagagat cacatacagg ggaaaagccc 4561 tatgaatgta acacatgcag gaaaaccttc tctcaaaagt caaatctcat tgtacatcag 4621 agaacacaca taggagaaaa accttatgaa ggatcccacc atcaccatca ccatcaccat 4681 tagcggccgc gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg 4741 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg 4801 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt 4861 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt 4921 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc 4981 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt 5041 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt 5101 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc 5161 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag 5221 ctgtgaccgg cgcctacgct agacgccacc atggagagcg acgagagcgg cctgcccgcc 5281 atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga gctggtgggc 5341 ggcggagagg gcacccccaa gcagggccgc atgaccaaca agatgaagag caccaaaggc 5401 gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt ctaccacttc 5461 ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa cggcggctac 5521 accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac 5581 cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag 5641 gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac 5701 cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc 5761 ggctactaca gcttcgtggt ggacagccac atgcacttca agagcgccat ccaccccagc 5821 atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagct gcacagcaac 5881 accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc cttcgccaga 5941 tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg ctccaccgga 6001 tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct 6061 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta 6121 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac 6181 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac 6241 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag 6301 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt 6361 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag 6421 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc 6481 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg 6541 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc 6601 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga 6661 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct 6721 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt 6781 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg 6841 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact 6901 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct 6961 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg 7021 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc 7081 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc 7141 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc 7201 cgccttcgcc ctcagacgag tcggatctcc ctttggccgc ctccccgcct ggtaccttta 7261 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 7321 ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct 7381 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 7441 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 7501 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 7561 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 7621 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 7681 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 7741 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt 7801 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 7861 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg 7921 gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 7981 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 8041 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 8101 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 8161 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 8221 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 8281 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 8341 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 8401 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 8461 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 8521 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 8581 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 8641 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 8701 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 8761 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 8821 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 8881 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 8941 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 9001 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 9061 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 9121 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 9181 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 9241 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 9301 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 9361 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 9421 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 9481 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 9541 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 9601 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 9661 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 9721 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 9781 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 9841 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 9901 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 9961 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 10021 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 10081 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 10141 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 10201 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 10261 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag 10321 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga 10381 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat 10441 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat 10501 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca 10561 agctg //