LOCUS V041701 9538 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V041701 VERSION V041701 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..4392 /label="ZNF418(NM_001317027)" /note="ZNF418(NM_001317027)" /gene="ZNF418" CDS 4399..4464 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 4508..4719 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4732..5000 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 5027..5626 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5633..6221 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6295..6528 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 6600..6721 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 6740..6875 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(6913..6929) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6937..6953 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6961..6991) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7006..7027 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7315..7903) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8074..8934) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8935..9039) /gene="bla" /label="AmpR promoter" primer_bind 9513..9529 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catgcaagaa ggccttcata gaatgacttg ggactgcatt 2341 gctattggga gccatgagaa ttctgtgcag ggcactgtgg catttgaaga tgtggctgtg 2401 aacttttccc aggaggagtg gagtctcctt agtgaggttc agagatgcct ttaccatgac 2461 gtgatgctgg agaactgggt acttatatcc tccctgggtt gttggtgtgg atcagaagat 2521 gaggaggcac cttctaagaa gagcatttct atacaaagag tgtctcaggt cagcactcct 2581 ggggcaggtg tgtctcccaa gaaggcccac tcttgtgaaa tgtgtggcgc gatcttggga 2641 gacattttgc acttggcaga tcatcagggg acacatcaca agcagaaact gcacaggtgt 2701 gaggcatggg ggaataaatt gtatgatagt tcaaaccgtc cgcaccagaa tcagtacctt 2761 ggagagaaac cctatagaag cagtgttgag gaagcattgt ttgtgaagag gtgtaagttc 2821 catgtgtcag aggagtcatc tatcttcatt cagagtggaa aggacttttt gcccagctca 2881 ggattactgc tgcaggaggc cactcacact ggggagaagt caaacagcaa acctgagtgt 2941 gagtctccct ttcagtgggg agatactcat tacagctgtg gagaatgcat gaaacattct 3001 agcaccaaac acgtatttgt tcaacagcag agacttccct ctagagagga atgttattgc 3061 tgggaatgtg ggaaatcctt tagcaaatat gatagcgtca gtaatcatca gagagttcac 3121 actgggaaaa gaccttatga atgtggagaa tgtgggaaat cttttagtca taagggcagc 3181 cttgttcagc atcagcgagt tcacactggg aaaagacctt atgaatgtgg agaatgtggg 3241 aaatctttta gtcataaggg cagccttgtt cagcatcagc gagttcatac tggagaaaga 3301 ccttatgagt gtggagaatg tgggaaatct tttagtcaaa atggtactct cattaaacat 3361 caacgagttc acactggaga aagaccttat gagtgtgaag aatgtgggaa atgttttact 3421 cagaagggca atctcattca acatcaacga ggtcacacta gtgaaagacc ttatgagtgt 3481 gaagaatgtg gaaaatgttt tagtcaaaag ggcaccctaa ctgaacatca tcgagttcac 3541 actagagaac gaccttatga gtgtggagaa tgtgggaaat cttttagtcg aaagggacac 3601 cttaggaacc atcagcgagg tcacactgga gaaagacctt acgagtgtgg agaatgtggg 3661 aaatctttta gtcgaaaggg caacctcatt cagcatcagc gaagccacac tggagaaagg 3721 ccttatgagt gtagagagtg taggaaatta tttaggggca agtcccacct cattgaacac 3781 cagagagttc acactggaga aaggccatat gaatgtaatg aatgtgggaa atcatttcaa 3841 gacagctctg ggtttcgtgt tcatcagaga gttcacactg gagaaaaacc gtttgagtgt 3901 agtgaatgtg ggaagtcatt tcctcaaagc tgttccctcc ttcgacatcg gagagttcat 3961 actggagaaa ggccttatga atgtggagaa tgtggaaagt catttcatca gagctcttcc 4021 ctccttcgac atcagaaaac tcacactgca gaaagacctt atgagtgcag agaatgtggg 4081 aaattcttct ccagtctcct tgaacacagg agagttcaca ctggagaaag gccttatgaa 4141 tgcagggaat gtggaaaaac atttactcga aggtctgcgc attttaaaca tcagagactt 4201 catactcgag gaaagcctta cgagtgcagc gaatgtggga aatcctttgc tgaaaccttc 4261 agtcttactg aacacaggag agtacacact ggagaaaggc cttatgagtg cagtgaatgt 4321 ggaaaatcat ttcatcgaag ctcttctctc cttcgacatc agagagttca cacagaaaga 4381 agtccttaca agggatccga ctacaaagac catgacggtg attataaaga tcatgacatc 4441 gactacaagg atgacgatga caagtagcgg ccgcgaagga tctgcgatcg ctccggtgcc 4501 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc 4561 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac 4621 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg 4681 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc 4741 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc 4801 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta 4861 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc 4921 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct 4981 gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagatga ccgagtacaa 5041 gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc 5101 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg 5161 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg 5221 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg 5281 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc 5341 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct 5401 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct 5461 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc 5521 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc 5581 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgagtcg acaatcaacc 5641 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac 5701 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt 5761 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt 5821 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg 5881 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac 5941 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac 6001 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt 6061 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc 6121 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg 6181 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggtacct ttaagaccaa 6241 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag 6301 ggctaattca ctcccaacga aaataagatc tgctttttgc ttgtactggg tctctctggt 6361 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 6421 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 6481 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat 6541 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga 6601 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 6661 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 6721 atcatgtctg gctctagcta tcccgcccct aactccgccc agttccgccc attctccgcc 6781 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct 6841 attccagaag tagtgaggag gcttttttgg aggcctagac ttttgcagag acggcccaaa 6901 ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 6961 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 7021 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 7081 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 7141 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 7201 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 7261 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 7321 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 7381 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 7441 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 7501 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 7561 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 7621 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 7681 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 7741 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 7801 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 7861 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 7921 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 7981 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 8041 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 8101 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 8161 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 8221 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 8281 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 8341 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 8401 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 8461 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 8521 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 8581 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 8641 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 8701 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 8761 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 8821 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 8881 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 8941 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 9001 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 9061 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 9121 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 9181 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 9241 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat 9301 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata 9361 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg 9421 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg 9481 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg //