LOCUS       V044160                12193 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044160
VERSION     V044160
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..6219
                     /label="ZNF532(NM_018181)"
                     /note="ZNF532(NM_018181)"
                     /gene="ZNF532"
     CDS             6226..6291
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6335..6546
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6559..6827
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6856..6865
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6886..7548
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7618..7671
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7672..8271
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8272..8860
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8934..9167
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9239..9360
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9400..9535
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9568..9584)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9592..9608
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9616..9646)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9661..9682
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9970..10558)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10729..11589)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11590..11694)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12168..12184
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga ccatggggga tatgaagacc
     2341 ccagactttg atgacctcct ggcagcattt gacatcccag atatggtcga tcctaaagca
     2401 gctattgagt ctggacacga tgaccatgaa agccacatga agcagaatgc tcacggagag
     2461 gatgactccc acgcaccatc atcttctgat gtgggtgtca gcgttatcgt caagaatgtt
     2521 cggaacattg actcttccga gggcggggag aaagacggcc acaaccccac tggcaatggc
     2581 ttacataatg ggtttctcac agcatcctcc cttgacagtt acagtaaaga tggagcaaag
     2641 tccttgaaag gagatgtgcc tgcctctgag gtgacactga aagactcgac attcagccag
     2701 tttagcccga tctccagtgc tgaagagttt gatgacgacg agaagattga ggtggatgac
     2761 ccccctgaca aggaggacat gcgatcaagc ttcaggtcga atgtgttgac ggggtcggct
     2821 ccccagcagg actacgataa gctgaaggca ctcggagggg aaaactccag caaaactgga
     2881 ctctctacgt caggcaatgt ggagaaaaac aaagctgtta agagagaaac agaagccagt
     2941 tctataaacc tgagtgttta tgaacctttt aaagtcagaa aagcagagga taaattgaag
     3001 gaaagctctg acaaggtgct ggaaaacaga gtcctagatg ggaagctgag ctccgagaag
     3061 aatgacacca gcctccccag cgttgcgcca tcaaagacaa agtcgtcctc caagctctcg
     3121 tcctgcatcg ctgccatcgc ggctctcagc gctaaaaagg cggcttcaga ctcctgcaaa
     3181 gaaccagtgg ccaattcgag ggaatcctcc ccgttaccaa aagaagtaaa tgacagtccg
     3241 agagccgctg acaagtctcc tgaatcccag aatctcatcg acgggaccaa aaaaccatcc
     3301 ctgaagcaac cggatagtcc cagaagcatc tcaagtgaga acagcagcaa aggatccccg
     3361 tcctctcccg cagggtccac accagcaatc cccaaagtcc gcataaaaac cattaagaca
     3421 tcttctgggg aaatcaagag aacagtgacc agggtattgc cagaagtgga tcttgactct
     3481 ggaaagaaac cttccgagca gacagcgtcc gtgatggcct ctgtgacatc ccttctgtcg
     3541 tctccagcat cagccgccgt cctttcctct ccccccaggg cgcctctcca gtctgcggtc
     3601 gtgaccaatg cagtttcccc tgcagagctc acccccaaac aggtcacaat caagcctgtg
     3661 gctactgctt tcctcccagt gtctgctgtg aagacggcag gatcccaagt cattaatttg
     3721 aagctcgcta acaacaccac ggtgaaagcc acggtcatat ctgctgcctc tgtccagagt
     3781 gccagcagcg ccatcattaa agctgccaac gccatccagc agcaaactgt cgtggtgccg
     3841 gcatccagcc tggccaatgc caaactcgtg ccaaagactg tgcaccttgc caaccttaac
     3901 cttttgcctc agggtgccca ggccacctct gaactccgcc aagtgctaac caaacctcag
     3961 caacaaataa agcaggcaat aatcaatgca gcagcctcgc aaccccccaa aaaggtgtct
     4021 cgagtccagg tggtgtcgtc cttgcagagt tctgtggtgg aagctttcaa caaggtgctg
     4081 agcagtgtca atccagtccc tgtttacatc ccaaacctca gtcctcccgc caatgcaggg
     4141 atcacgttac cgacgcgtgg gtacaagtgc ttggagtgtg gggactcctt tgcacttgaa
     4201 aagagtctga cccagcacta cgacagacgg agcgtgcgca tcgaagtaac gtgcaaccat
     4261 tgtacaaaga acctcgtttt ttacaacaaa tgcagcctcc tttcccatgc ccgtgggcat
     4321 aaggagaaag gggtggtaat gcaatgctcc cacttaattt taaagccagt cccagcagat
     4381 caaatgatag tttctccgtc aagcaatact tccacttcaa cttccactct tcagagccct
     4441 gtgggagctg gcacacacac tgtcacaaaa attcagtctg gcataactgg gacagtcata
     4501 tcggctcctt caagcactcc catcacccca gccatgcccc tagatgaaga cccctccaaa
     4561 ctgtgtagac atagtctaaa atgtttggag tgtaatgaag tcttccagga cgagacatca
     4621 ctggctacac atttccagca ggctgcagat acgagtggac aaaagacttg cactatctgc
     4681 cagatgctgc ttcctaacca gtgcagttat gcatcacacc agagaatcca tcagcacaaa
     4741 tctccctaca cctgccctga gtgtggggcc atctgcaggt cggtgcactt ccagacccac
     4801 gtcaccaaga actgtctgca ctacacgagg agagttggtt ttcgatgtgt gcattgcaat
     4861 gttgtgtact ctgatgtggc tgctctgaag tctcacattc aaggttctca ctgtgaagtc
     4921 ttctacaagt gtcctatttg tccaatggcg tttaagtctg ccccaagcac acattcccac
     4981 gcctacacac agcatcctgg catcaagata ggagaaccaa aaataatata taagtgttcc
     5041 atgtgcgaca ctgtgttcac cctgcaaacc ttgctgtatc gccactttga ccaacacatt
     5101 gaaaaccaga aggtgtctgt tttcaagtgt ccagactgtt ctcttttata tgcacagaag
     5161 caacttatga tggaccatat caagtctatg catggaacat tgaaaagtat tgaagggcct
     5221 ccaaacttgg gtataaactt gcctttgagc attaagcctg caactcaaaa ttcagcaaat
     5281 cagaacaaag aggacaccaa atccatgaat gggaaagaga aattggaaaa gaaatctcca
     5341 tctcctgtga aaaaatcaat ggaaaccaag aaagtggcca gtcctgggtg gacgtgttgg
     5401 gagtgtgact gcctgttcat gcagagagat gtgtacatat cccacgtgag gaaggagcac
     5461 gggaagcaaa tgaagaaaca cccctgccgc cagtgtgaca agtctttcag ctcgtcccac
     5521 agcctgtgcc ggcacaaccg gatcaagcac aaaggcatca ggaaagtgta cgcctgctcg
     5581 cactgcccag actccagacg tacctttacc aaacgtttga tgctggagaa gcacgtccag
     5641 ctgatgcatg gcatcaagga ccctgacctg aaagaaatga cagatgccac caatgaggag
     5701 gaaacagaaa taaaagaaga cactaaggtc cccagtccca agcggaagtt ggaagaacca
     5761 gttctggagt tcaggcctcc ccgaggagca atcactcaac cactgaaaaa gctgaaaatc
     5821 aatgttttta aggttcacaa gtgtgccgtg tgtggcttca ccaccgaaaa cctgctgcaa
     5881 ttccacgaac acatccctca gcacaaatcg gatggttctt cctaccagtg ccgggagtgt
     5941 ggcctctgct acacgtctca cgtctctctg tccaggcacc tcttcatcgt acacaagtta
     6001 aaggaacctc agccagtgtc caagcaaaat ggggctgggg aagataacca acaggagaac
     6061 aaacccagcc acgaggatga atcccctgat ggcgccgtgt cagacagaaa gtgcaaagtg
     6121 tgcgcaaaaa cttttgaaac tgaagctgcc ttaaatactc acatgcggac acacggcatg
     6181 gccttcatca aatccaaaag gatgagctca gccgagaaag gatccgacta caaagaccat
     6241 gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa gtagcggccg
     6301 cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     6361 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg
     6421 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     6481 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     6541 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga
     6601 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga
     6661 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg
     6721 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct
     6781 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg
     6841 gcgcctacgc tagacgccac catggagagc gacgagagcg gcctgcccgc catggagatc
     6901 gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg cggcggagag
     6961 ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg cgccctgacc
     7021 ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt cggcacctac
     7081 cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta caccaacacc
     7141 cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag
     7201 gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg
     7261 atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca ccccatgggc
     7321 gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg cggctactac
     7381 agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag catcctgcag
     7441 aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa caccgagctg
     7501 ggcatcgtgg agtaccagca cgccttcaag acccccatcg ccttcgccag atcccgcgct
     7561 cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg atctcgcgag
     7621 ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc tatgaccgag
     7681 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     7741 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     7801 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     7861 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     7921 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     7981 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     8041 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     8101 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     8161 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     8221 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg aaatcaacct
     8281 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     8341 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     8401 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     8461 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     8521 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     8581 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     8641 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     8701 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     8761 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     8821 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggtacctt taagaccaat
     8881 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     8941 gctaattcac tcccaacgaa gataagatct gctttttgct tgtactgggt ctctctggtt
     9001 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     9061 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     9121 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     9181 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     9241 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     9301 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     9361 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     9421 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     9481 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac
     9541 ttttgcagag accaaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc
     9601 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     9661 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     9721 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     9781 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     9841 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     9901 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     9961 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    10021 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    10081 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    10141 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    10201 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    10261 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    10321 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    10381 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
    10441 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    10501 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    10561 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    10621 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    10681 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    10741 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    10801 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    10861 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    10921 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    10981 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    11041 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    11101 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    11161 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    11221 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    11281 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    11341 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    11401 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    11461 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    11521 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    11581 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    11641 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    11701 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
    11761 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
    11821 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
    11881 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
    11941 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
    12001 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
    12061 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
    12121 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
    12181 cagtgccaag ctg
//
