LOCUS       V029465                11821 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029465
VERSION     V029465
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5847
                     /label="Zc3h6(NM_178404)"
                     /note="Zc3h6(NM_178404)"
                     /gene="Zc3h6"
     CDS             5854..5919
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5963..6174
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6187..6455
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6484..6493
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6514..7176
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7246..7299
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7300..7899
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7900..8488
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8562..8795
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8867..8988
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9028..9163
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9196..9212)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9220..9236
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9244..9274)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9289..9310
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9598..10186)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10357..11217)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11218..11322)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11796..11812
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga cagactctga acatgcaggg
     2341 cacgacagag aagatggtga attagaagat ggagaaatag atgatgcagg atttgaagaa
     2401 acacaagatc aggaagcaaa agaaaatgaa aagcaaaaaa atgagaaagc ctacagaaaa
     2461 tcaaggaaaa aacacaagaa agaaagagaa aagaaaaaat ccaaaaggag gaaacacgag
     2521 aaacataagc ataactctcc atctggtgat gatagttctg actacagcct ggactcagat
     2581 gttgaacgta tgcaaagttc ccgtaagaaa agaaccagct cctacaggga ttatgatgtt
     2641 ccattttctc agcatagacg tatatcagga agttacatga catcaaagaa gagtcaacat
     2701 aacaaaaaaa ctaatagtaa agaatatgct gagtccagtt tctacagtga tgactacttt
     2761 ggtaactaca gtgatgacaa ctttggtaac tatagtaacc aggaaggaga agaagatttc
     2821 tccagtcagc tgaaatacta tcgacagtcc caggagtcct cagggtcatc gttctctaag
     2881 gaatcaggga agaaactgag aagtaaagga agcccaccag gaactgaata tcggattaaa
     2941 agttttgatg ttagtcatgg acatcttttg ccgaagaaaa tcagaagaaa agaacactgt
     3001 ggggcacgag tcattaaagg gccttatgtc ttttcaggaa tggatgactt tcaagagtac
     3061 agcaaaccag ggaaaaagtg gaaagttatg actcaggagt ttattaacca gcacacagtg
     3121 gaacacaaag gaaaacagat ctgtaaatac ttcctggaag ggagatgtat taagggagat
     3181 cattgtaaat ttaatcatga tgcagaattg gagaagaaaa aggaggtctg caaatattat
     3241 ttacagggat attgtaccaa aggagagaac tgcatttaca tgcatagtga atttccttgc
     3301 aagttctatc atagtggagc aaagtgctac caaggagaca agtgtaagtt ttcccatgat
     3361 gatctgacta aagaaacaag gaaacttttg gacaaagtgt tgaatgcgga tgaagaactt
     3421 gtaaatgaag acgaaagaga attagaagaa cttaggaaac gcggcataac tcctctcccc
     3481 aaaccacccc cgggagtcgg ccttctcccg accccatcag agcattttcc gttttctgat
     3541 cctgaagatg attttgagac ggatctttct gatgatatga agaaaattcc atctcttttt
     3601 gaaatagttg taaaacctac tgtggattta gcacataaaa ttgggaagaa accaccagca
     3661 ttttacaaca gtacctcacc accaggacca cagtttgagg aaagcagtca ctgtccacag
     3721 cgtatgtaca gttctgagtc aagtccaggt ccaggatcta aagttcctca aggatgtgag
     3781 agtcctgtga ggcacccagg ctcccctgga caccaccctt gtgtaggacc tcctggtcca
     3841 ccaatgcaag agaacccatc tttgctaccc agttcatctg aaattgtagg ccctcatagt
     3901 caagctggag ggcttgttca gctggacacg ctaccaagca tgggtggagc ttaccattcc
     3961 ccgggctttc caggacactc ggtgaaagtc cctagagaga gtcacagctc tccagcttca
     4021 ctgtaccagc aaatgcctag tgagatgcaa cgcagtgccg actctgagtc catgcaaggc
     4081 tcagctgaat tttatgatga ttactaccca cagcatgctg cacataattt tcaaccacct
     4141 gataactctg ctgatgagat gtggcatgaa gagtttgcac agcagcagcc tcccattgct
     4201 cgtgacacag cacaccttgg gagtgggccc aattccagca gcagaatgac aagtcactgc
     4261 cctctgtctg cttcagggct ccctcctgca gtgcagagag ctcttttcat cccgttgact
     4321 cagagatacc aagaagatga agaaccagct ggcacccagc ctcatagagc atcaagcaag
     4381 gaagaagatg atacagctaa ctggtattcc agtagtgaag aggaagaagg aagtggtgtc
     4441 aagtcaatac tgaggacatt acagaaacaa acaggaacac tgagaaacca gcaactccct
     4501 cccacagagc tcagcgttcc tactgatcca agactcgcta aagagaaaag gaaaagaaat
     4561 caagtggttg acccaagact tcggactgtc ccaaggcaag atattaaaaa gccgcatgag
     4621 tctgtcccag tggaccttag acttgtgtgg gaccccaaga aattaagagg gaatggaggt
     4681 gctcctgggg gctcttcagc taggggagca gagtttgact tacgtcatac aaatgctggt
     4741 gcaaatcaca aatccaaaag aagagaggat gatgatgaag attcagaaag agaactaaga
     4801 gaaaaggcct tcttaatacc tttggattct tcacctggca tagtcctcca ggatccaagg
     4861 tcacagttaa gacaattcag tcatattaaa atggacatta ttctaaacaa acccaacttt
     4921 gcaaaacaca ttgtgtgggc tccagaagac ttacttccag tacctttacc taaacctgat
     4981 ccagtatctt caatcaactt acctctgccc ccacttatag ctgaccagag gctcaataga
     5041 ttatggaata caaagagtga tcatcagggt gccctgagcc ttgatccaac atcagcagcc
     5101 aaagccaaac tgagtctaac acacagagag ggctgcttag agcagtctgg agacttacac
     5161 agttcaggag gtaaactagg agaccctagg ctgcaaaaga attttgatcc taggcttcat
     5221 agactgccca atacagagtc tcatcaagtg actgcaaagg attcacactc atcaaggagt
     5281 gccctgccct tagccagatg gaaccctgct ttgtcacagt cctcaacagc agcacctatt
     5341 aatgttgctt ctgtgactcc tcccctgtat gcccccaaac tctcttcaga aggcctccca
     5401 ccgggaactt caagttcagt tcttagtggc attagtttgt atgatcctag ggataaaggt
     5461 tcgttatctg caatggagct atcaaccatt tcttcaggag aaaatacaga gagccagaaa
     5521 aaaagtggct taaaaaatag tgataaaaac cagccttctc ctggagaagt gatcgtacca
     5581 cagaacacta ctgcaaactt ggaagtccct gttgatgggc cagtagacat gcagacagat
     5641 attctcagga gtgctgataa ggttcaggtc cctgctgtgc acagtcttcc tattcaggcc
     5701 ttaacaggct tacttagacc tccatacagt gatccaaggc aggcaagaga accaggacag
     5761 gcaagcccaa ccccagatga agaaacagat gacaaacctc tgaaagaggt ttttaagacg
     5821 tttgatccaa ctgcatcacc gttttgtgga tccgactaca aagaccatga cggtgattat
     5881 aaagatcatg acatcgacta caaggatgac gatgacaagt agcggccgcg aaggatctgc
     5941 gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     6001 gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa
     6061 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     6121 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag
     6181 cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca
     6241 cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc
     6301 gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc
     6361 ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc
     6421 tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgcta
     6481 gacgccacca tggagagcga cgagagcggc ctgcccgcca tggagatcga gtgccgcatc
     6541 accggcaccc tgaacggcgt ggagttcgag ctggtgggcg gcggagaggg cacccccaag
     6601 cagggccgca tgaccaacaa gatgaagagc accaaaggcg ccctgacctt cagcccctac
     6661 ctgctgagcc acgtgatggg ctacggcttc taccacttcg gcacctaccc cagcggctac
     6721 gagaacccct tcctgcacgc catcaacaac ggcggctaca ccaacacccg catcgagaag
     6781 tacgaggacg gcggcgtgct gcacgtgagc ttcagctacc gctacgaggc cggccgcgtg
     6841 atcggcgact tcaaggtggt gggcaccggc ttccccgagg acagcgtgat cttcaccgac
     6901 aagatcatcc gcagcaacgc caccgtggag cacctgcacc ccatgggcga taacgtgctg
     6961 gtgggcagct tcgcccgcac cttcagcctg cgcgacggcg gctactacag cttcgtggtg
     7021 gacagccaca tgcacttcaa gagcgccatc caccccagca tcctgcagaa cgggggcccc
     7081 atgttcgcct tccgccgcgt ggaggagctg cacagcaaca ccgagctggg catcgtggag
     7141 taccagcacg ccttcaagac ccccatcgcc ttcgccagat cccgcgctca gtcgtccaat
     7201 tctgccgtgg acggcaccgc cggacccggc tccaccggat ctcgcgaggg cagaggaagt
     7261 cttctaacat gcggtgacgt ggaggagaat cccggcccta tgaccgagta caagcccacg
     7321 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     7381 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     7441 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     7501 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     7561 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     7621 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     7681 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     7741 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     7801 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     7861 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa atcaacctct ggattacaaa
     7921 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     7981 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     8041 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     8101 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     8161 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     8221 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     8281 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     8341 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     8401 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     8461 cggatctccc tttgggccgc ctccccgcct ggtaccttta agaccaatga cttacaaggc
     8521 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     8581 ccaacgaaga taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     8641 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     8701 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     8761 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     8821 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     8881 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     8941 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     9001 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     9061 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     9121 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac
     9181 caaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     9241 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     9301 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     9361 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     9421 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     9481 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     9541 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     9601 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     9661 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     9721 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     9781 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     9841 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     9901 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     9961 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
    10021 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
    10081 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
    10141 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
    10201 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
    10261 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
    10321 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
    10381 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
    10441 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
    10501 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
    10561 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
    10621 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
    10681 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    10741 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    10801 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    10861 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    10921 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    10981 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    11041 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    11101 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    11161 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    11221 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    11281 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    11341 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
    11401 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
    11461 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
    11521 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
    11581 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
    11641 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
    11701 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
    11761 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct
    11821 g
//
