LOCUS V033483 9128 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V033483 VERSION V033483 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1798..1915 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 1960..2171 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 2184..2452 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 2498..3268 /label="Sirt3(NM_022433)" /note="Sirt3(NM_022433)" /gene="Sirt3" promoter 3287..3786 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 3831..4493 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /transl_table=1 /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" /label="CopGFP" CDS 4563..4616 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 4617..5216 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5223..5811 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 5885..6118 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 6190..6311 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 6330..6465 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(6503..6519) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6527..6543 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6551..6581) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6596..6617 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6905..7493) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7664..8524) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8525..8629) /gene="bla" /label="AmpR promoter" primer_bind 9103..9119 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca 1981 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc 2041 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg 2101 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg 2161 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta 2221 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct 2281 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc 2341 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc 2401 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt 2461 gaccggcgcc tactctagag ctagcgaatt cgccaccatg gtgggggccg gcatcagcac 2521 acccagtggc atcccggact tcagatcccc agggagcggc ctctacagca accttcagca 2581 gtatgacatc ccgtaccctg aagccatctt tgaacttggc tttttctttc acaaccccaa 2641 gccctttttc atgttggcca aggagctgta ccctgggcac tacaggccca atgtcactca 2701 ctacttcctg aggctcctcc acgacaagga gctgcttctg cggctctata cacagaacat 2761 cgacgggctt gagagagcat ctgggatccc tgcctcaaag ctggttgaag cccacgggac 2821 ctttgtaaca gctacatgca cggtctgtcg aaggtccttc ccaggggaag acatatgggc 2881 tgatgtgatg gcggacaggg tgccccgctg ccctgtctgt actggcgttg tgaaacccga 2941 cattgtgttc tttggggagc agctgcctgc aaggttccta ctccatatgg ctgacttcgc 3001 tttggcagat ctgctactca ttcttgggac ctccctggag gtggagcctt ttgccagctt 3061 gtctgaagca gtacagaaat cagtgccccg actgctcatc aatcgagact tggtggggcc 3121 gttcgttctg agtcctcgaa ggaaagatgt ggtccagcta ggggatgtag ttcatggtgt 3181 ggaaaggctg gtggacctcc tggggtggac acaagaactg ctggatctta tgcagcggga 3241 acgtggcaag ctggatggac aggacagata aggatccgcg gccgctgggt aggggaggcg 3301 cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac 3361 acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg 3421 ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc 3481 gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc 3541 gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa 3601 tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc 3661 gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca 3721 ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc 3781 ctttcgacct gcagcccaag cttaccatgg agagcgacga gagcggcctg cccgccatgg 3841 agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg gtgggcggcg 3901 gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc aaaggcgccc 3961 tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac cacttcggca 4021 cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc ggctacacca 4081 acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc agctaccgct 4141 acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc cccgaggaca 4201 gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac ctgcacccca 4261 tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc gacggcggct 4321 actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac cccagcatcc 4381 tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg 4441 agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc gccagatccc 4501 gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc accggatctc 4561 gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc ggccctatga 4621 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca 4681 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc 4741 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg 4801 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg 4861 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc 4921 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg 4981 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg 5041 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga 5101 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg 5161 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgagtcg 5221 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg 5281 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc 5341 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt 5401 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca 5461 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc 5521 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc 5581 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc 5641 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc 5701 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc 5761 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggtacct 5821 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg 5881 ggactggaag ggctaattca ctcccaacga aaataagatc tgctttttgc ttgtactggg 5941 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg 6001 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt 6061 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt 6121 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag 6181 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 6241 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 6301 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc agttccgccc 6361 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 6421 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac ttttgcagag 6481 acggcccaaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 6541 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag 6601 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 6661 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 6721 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 6781 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 6841 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 6901 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 6961 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 7021 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 7081 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 7141 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 7201 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 7261 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 7321 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag 7381 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 7441 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 7501 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 7561 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 7621 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 7681 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 7741 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 7801 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 7861 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 7921 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 7981 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 8041 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 8101 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 8161 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 8221 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 8281 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 8341 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 8401 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 8461 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 8521 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 8581 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 8641 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata 8701 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac 8761 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag 8821 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat 8881 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa 8941 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc 9001 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc 9061 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg 9121 ccaagctg //