LOCUS       V021343                10668 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021343
VERSION     V021343
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2815..5319
                     /label="CMTR1(NM_015050)"
                     /note="CMTR1(NM_015050)"
                     /gene="CMTR1"
     misc_feature    5339..5911
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
                     /label="IRES"
     CDS             5913..6623
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK*"
                     /label="mCherry"
     misc_feature    6637..7225
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7432..8065
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8193..8209)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8217..8233
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8241..8271)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8286..8307
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8595..9183)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9354..10214)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10215..10319)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    10367..10501
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatgaag
     2821 aggagaactg acccagaatg cactgccccc atcaagaaac agaaaaaaag agttgcagag
     2881 cttgccctga gcctcagctc cacgtccgat gatgaacctc cctcctctgt cagtcatgga
     2941 gcaaaagcat ctactacaag ccttagtggg tctgatagtg agaccgaggg gaaacaacac
     3001 agctctgact cttttgacga tgcattcaaa gcagactctc ttgtggaagg aacttcttct
     3061 cgctattcca tgtataatag cgtctcccag aagcttatgg ccaagatggg cttcagggaa
     3121 ggtgaaggat tgggtaaata cagccagggt cggaaggaca tcgttgaggc ttccagtcag
     3181 aaaggtcgaa gaggcttggg tctgacactc cggggctttg accaggagct gaacgtggac
     3241 tggcgagatg agccagagcc cagtgcttgt gagcaggtgt catggtttcc agaatgtacc
     3301 actgaaattc ctgacactca ggaaatgagc gattggatgg tggtgggaaa gagaaagatg
     3361 attattgaag atgaaacaga gttttgtggg gaagagctgc ttcacagtgt gttgcagtgt
     3421 aagagcgtgt ttgatgtctt ggatggggaa gagatgcggc gagctcggac tcgggccaat
     3481 ccctatgaga tgatccgagg agtcttcttt ctaaacaggg cagcaatgaa gatggctaac
     3541 atggattttg tatttgatcg catgttcaca aatccgcggg actcttatgg gaagccactg
     3601 gtgaaggacc gggaagctga gcttctgtac tttgctgatg tctgcgcagg cccaggtggc
     3661 ttctcagagt atgtgctgtg gaggaagaag tggcatgcaa agggctttgg aatgactttg
     3721 aagggcccta atgacttcaa gctggaggac ttctactctg cttccagtga actcttcgaa
     3781 ccctactatg gtgagggtgg gattgatgga gatggagata tcacccgccc agagaacatc
     3841 tctgcttttc ggaattttgt cctggataac acagatcgca agggtgtcca ttttctgatg
     3901 gctgatgggg gtttctcggt ggaggggcag gagaacctgc aggagatcct cagcaagcag
     3961 ctgcttctgt gtcagttcct catggcgctg tccattgtcc ggacaggagg ccacttcatc
     4021 tgtaaaacct ttgacctgtt cacaccgttt agtgtggggc ttgtctacct gctgtactgc
     4081 tgctttgaac gagtttgtct cttcaagcct attaccagcc gtcctgccaa ctcagagagg
     4141 tatgtggtgt gcaagggcct gaaggtgggc atagatgatg ttcgggatta cctcttcgca
     4201 gtgaatatta aactcaatca gctgcggaac acggattccg acgtcaactt ggtggtcccc
     4261 ctggaggtga tcaagggaga ccatgaattt actgactaca tgatacggtc caatgagagc
     4321 cactgtagtc tgcagatcaa agctctggcg aaaatccatg cctttgttca agacacgaca
     4381 ctgagtgagc ctcgacaggc agagatacgg aaggagtgcc tccgactctg ggggatccca
     4441 gaccaggctc gtgtggctcc ttcttcctcc gaccctaaat cgaagttctt tgagctaatc
     4501 cagggcactg agattgacat cttcagctac aagcccacac tgctcacctc taaaaccctg
     4561 gagaagatcc gccctgtgtt tgactaccgc tgcatggtat ctggcagtga gcagaagttc
     4621 ctcatcggcc tggggaaatc ccagatctac acatgggatg gccgccagtc agaccgctgg
     4681 atcaagctag acctgaagac agagctgccc cgggacactc tgctatctgt ggaaattgtg
     4741 catgagctga aaggggaggg gaaggcccag aggaagatca gtgccatcca catcctcgat
     4801 gtccttgtgc tgaatggcac cgacgttcgg gagcagcact ttaaccagcg aattcagctt
     4861 gccgagaaat ttgtgaaagc cgtttccaag cctagtcggc ccgacatgaa tcccatcagg
     4921 gtgaaggagg tgtacagact ggaagagatg gagaagattt ttgtcaggtt ggagatgaag
     4981 atcatcaagg gctccagtgg caccccaaag ctcagctaca cagggcgtga tgaccggcac
     5041 tttgtaccca tgggcctcta catcgtcagg acagtgaatg agccctggac tatgggattc
     5101 agcaaaagct tcaagaagaa gttcttctac aacaagaaaa ccaaggactc tacttttgac
     5161 ctccctgcag actccattgc cccatttcac atttgctact atggccggct cttctgggag
     5221 tggggggatg gcattcgtgt gcatgactcc cagaagcccc aggaccagga caagctgtcc
     5281 aaggaggacg tcctctcctt catccagatg cacagggcct aagcggccgc ggatcccgcc
     5341 cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
     5401 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
     5461 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
     5521 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
     5581 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
     5641 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
     5701 ttgtgagttg gatagttgtg gaaagagtca aatggctcac ctcaagcgta ttcaacaagg
     5761 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
     5821 catgctttac atgtgtttag tcgaggttaa aaaacgtcta ggccccccga accacgggga
     5881 cgtggttttc ctttgaaaaa cacgatgata atatggtgag caagggcgag gaggataaca
     5941 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
     6001 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
     6061 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
     6121 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc
     6181 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
     6241 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc
     6301 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
     6361 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc
     6421 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga
     6481 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca
     6541 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg
     6601 gcatggacga gctgtacaag tgaacgcgtc tggaacaatc aacctctgga ttacaaaatt
     6661 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     6721 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     6781 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     6841 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     6901 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     6961 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     7021 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     7081 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     7141 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     7201 atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac ctagaaaaac
     7261 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag
     7321 cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga
     7381 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga ctggaagggc
     7441 taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     7501 acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg
     7561 gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag
     7621 agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     7681 tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc
     7741 cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact
     7801 ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat
     7861 aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
     7921 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     7981 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     8041 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     8101 taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattgcta gcgtttaccg
     8161 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     8221 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     8281 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     8341 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     8401 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     8461 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     8521 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     8581 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     8641 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     8701 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     8761 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     8821 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     8881 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     8941 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     9001 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     9061 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     9121 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     9181 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     9241 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     9301 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     9361 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     9421 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     9481 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     9541 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     9601 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     9661 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     9721 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     9781 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     9841 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     9901 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     9961 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    10021 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    10081 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    10141 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    10201 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    10261 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    10321 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatcaact tgtttattgc
    10381 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    10441 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
    10501 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac
    10561 cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc
    10621 attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagt
//
