LOCUS V021343 10668 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V021343 VERSION V021343 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2815..5319 /label="CMTR1(NM_015050)" /note="CMTR1(NM_015050)" /gene="CMTR1" misc_feature 5339..5911 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" /label="IRES" CDS 5913..6623 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /transl_table=1 /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK*" /label="mCherry" misc_feature 6637..7225 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 7432..8065 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(8193..8209) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 8217..8233 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(8241..8271) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 8286..8307 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8595..9183) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(9354..10214) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(10215..10319) /gene="bla" /label="AmpR promoter" polyA_signal 10367..10501 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatgaag 2821 aggagaactg acccagaatg cactgccccc atcaagaaac agaaaaaaag agttgcagag 2881 cttgccctga gcctcagctc cacgtccgat gatgaacctc cctcctctgt cagtcatgga 2941 gcaaaagcat ctactacaag ccttagtggg tctgatagtg agaccgaggg gaaacaacac 3001 agctctgact cttttgacga tgcattcaaa gcagactctc ttgtggaagg aacttcttct 3061 cgctattcca tgtataatag cgtctcccag aagcttatgg ccaagatggg cttcagggaa 3121 ggtgaaggat tgggtaaata cagccagggt cggaaggaca tcgttgaggc ttccagtcag 3181 aaaggtcgaa gaggcttggg tctgacactc cggggctttg accaggagct gaacgtggac 3241 tggcgagatg agccagagcc cagtgcttgt gagcaggtgt catggtttcc agaatgtacc 3301 actgaaattc ctgacactca ggaaatgagc gattggatgg tggtgggaaa gagaaagatg 3361 attattgaag atgaaacaga gttttgtggg gaagagctgc ttcacagtgt gttgcagtgt 3421 aagagcgtgt ttgatgtctt ggatggggaa gagatgcggc gagctcggac tcgggccaat 3481 ccctatgaga tgatccgagg agtcttcttt ctaaacaggg cagcaatgaa gatggctaac 3541 atggattttg tatttgatcg catgttcaca aatccgcggg actcttatgg gaagccactg 3601 gtgaaggacc gggaagctga gcttctgtac tttgctgatg tctgcgcagg cccaggtggc 3661 ttctcagagt atgtgctgtg gaggaagaag tggcatgcaa agggctttgg aatgactttg 3721 aagggcccta atgacttcaa gctggaggac ttctactctg cttccagtga actcttcgaa 3781 ccctactatg gtgagggtgg gattgatgga gatggagata tcacccgccc agagaacatc 3841 tctgcttttc ggaattttgt cctggataac acagatcgca agggtgtcca ttttctgatg 3901 gctgatgggg gtttctcggt ggaggggcag gagaacctgc aggagatcct cagcaagcag 3961 ctgcttctgt gtcagttcct catggcgctg tccattgtcc ggacaggagg ccacttcatc 4021 tgtaaaacct ttgacctgtt cacaccgttt agtgtggggc ttgtctacct gctgtactgc 4081 tgctttgaac gagtttgtct cttcaagcct attaccagcc gtcctgccaa ctcagagagg 4141 tatgtggtgt gcaagggcct gaaggtgggc atagatgatg ttcgggatta cctcttcgca 4201 gtgaatatta aactcaatca gctgcggaac acggattccg acgtcaactt ggtggtcccc 4261 ctggaggtga tcaagggaga ccatgaattt actgactaca tgatacggtc caatgagagc 4321 cactgtagtc tgcagatcaa agctctggcg aaaatccatg cctttgttca agacacgaca 4381 ctgagtgagc ctcgacaggc agagatacgg aaggagtgcc tccgactctg ggggatccca 4441 gaccaggctc gtgtggctcc ttcttcctcc gaccctaaat cgaagttctt tgagctaatc 4501 cagggcactg agattgacat cttcagctac aagcccacac tgctcacctc taaaaccctg 4561 gagaagatcc gccctgtgtt tgactaccgc tgcatggtat ctggcagtga gcagaagttc 4621 ctcatcggcc tggggaaatc ccagatctac acatgggatg gccgccagtc agaccgctgg 4681 atcaagctag acctgaagac agagctgccc cgggacactc tgctatctgt ggaaattgtg 4741 catgagctga aaggggaggg gaaggcccag aggaagatca gtgccatcca catcctcgat 4801 gtccttgtgc tgaatggcac cgacgttcgg gagcagcact ttaaccagcg aattcagctt 4861 gccgagaaat ttgtgaaagc cgtttccaag cctagtcggc ccgacatgaa tcccatcagg 4921 gtgaaggagg tgtacagact ggaagagatg gagaagattt ttgtcaggtt ggagatgaag 4981 atcatcaagg gctccagtgg caccccaaag ctcagctaca cagggcgtga tgaccggcac 5041 tttgtaccca tgggcctcta catcgtcagg acagtgaatg agccctggac tatgggattc 5101 agcaaaagct tcaagaagaa gttcttctac aacaagaaaa ccaaggactc tacttttgac 5161 ctccctgcag actccattgc cccatttcac atttgctact atggccggct cttctgggag 5221 tggggggatg gcattcgtgt gcatgactcc cagaagcccc aggaccagga caagctgtcc 5281 aaggaggacg tcctctcctt catccagatg cacagggcct aagcggccgc ggatcccgcc 5341 cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg 5401 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga 5461 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa 5521 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa 5581 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct 5641 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg 5701 ttgtgagttg gatagttgtg gaaagagtca aatggctcac ctcaagcgta ttcaacaagg 5761 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca 5821 catgctttac atgtgtttag tcgaggttaa aaaacgtcta ggccccccga accacgggga 5881 cgtggttttc ctttgaaaaa cacgatgata atatggtgag caagggcgag gaggataaca 5941 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc 6001 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca 6061 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt 6121 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc 6181 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg 6241 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc 6301 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg 6361 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc 6421 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga 6481 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca 6541 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg 6601 gcatggacga gctgtacaag tgaacgcgtc tggaacaatc aacctctgga ttacaaaatt 6661 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct 6721 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg 6781 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc 6841 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt 6901 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc 6961 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg 7021 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg 7081 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc 7141 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg 7201 atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac ctagaaaaac 7261 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag 7321 cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga 7381 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga ctggaagggc 7441 taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct 7501 acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg 7561 gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag 7621 agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag 7681 tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc 7741 cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact 7801 ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat 7861 aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag 7921 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt 7981 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt 8041 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta 8101 taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattgcta gcgtttaccg 8161 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 8221 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt 8281 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 8341 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg 8401 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 8461 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 8521 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 8581 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 8641 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 8701 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 8761 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 8821 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 8881 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 8941 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 9001 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg 9061 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 9121 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 9181 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 9241 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 9301 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 9361 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 9421 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 9481 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 9541 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 9601 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 9661 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 9721 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 9781 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 9841 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 9901 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 9961 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 10021 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 10081 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 10141 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 10201 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 10261 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 10321 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatcaact tgtttattgc 10381 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 10441 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat 10501 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac 10561 cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc 10621 attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagt //