LOCUS       V021384                11172 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021384
VERSION     V021384
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2815..5823
                     /label="MOV10(NM_020963)"
                     /note="MOV10(NM_020963)"
                     /gene="MOV10"
     misc_feature    5843..6415
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
                     /label="IRES"
     CDS             6417..7127
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK*"
                     /label="mCherry"
     misc_feature    7141..7729
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7936..8569
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8697..8713)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8721..8737
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8745..8775)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8790..8811
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9099..9687)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9858..10718)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10719..10823)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    10871..11005
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatgccc
     2821 agtaagttca gctgccggca gctccgggag gcgggccagt gtttcgagag tttcctggtc
     2881 gttcggggac tggacatgga gacagatcgc gagcggctgc ggaccattta taaccgcgac
     2941 ttcaagatca gctttgggac ccccgcccct ggcttctcct ccatgctgta tggaatgaag
     3001 attgcaaatc tggcctacgt caccaagact cgggtcaggt tcttcagact cgaccgctgg
     3061 gccgacgtgc ggttcccaga aaagaggaga atgaagctgg ggtcagatat cagcaaacac
     3121 cacaagtcac tgctagccaa gatcttttat gacagggctg agtatcttca tgggaaacat
     3181 ggtgtggatg tggaagtcca ggggccccat gaagcccgag atgggcagct ccttatccgc
     3241 ctggatttga accgcaaaga ggtgctgacc ctgaggcttc ggaatggcgg aacccagtct
     3301 gttaccctca ctcacctctt cccactctgc cggacacccc agtttgcttt ctacaatgaa
     3361 gaccaggagt tgccctgtcc actgggcccc ggtgaatgct atgaactcca tgtccattgt
     3421 aagaccagct ttgtgggcta cttcccagcc acagtgctct gggagctgct gggacctggg
     3481 gagtcgggtt cagaaggagc cggcacattc tacattgccc gcttcttggc tgccgtcgcc
     3541 cacagccccc tggctgcaca gctgaagccc atgactccct tcaagcggac ccggatcacc
     3601 ggaaaccctg tggtgaccaa tcggatagag gaaggagaga gacctgaccg cgctaagggc
     3661 tatgacctgg agttaagtat ggcgctgggg acatactacc cacctccccg cctcaggcag
     3721 ctgctcccca tgcttcttca gggaacaagt atcttcactg cccctaagga gatcgcagag
     3781 atcaaggccc agctggagac agccctgaag tggaggaact atgaggtgaa gctgcggctg
     3841 ctgctgcacc tggaggaact gcagatggag catgatatcc ggcactatga cctggagtcg
     3901 gtgcccatga cctgggaccc tgtggaccag aaccccaggc tgctcacgct ggaggttcct
     3961 ggagtgactg agagccgccc ctcagtgcta cggggcgacc acctgtttgc ccttttgtcc
     4021 tcggagacac accaggagga ccccatcaca tataagggct ttgtgcacaa ggtggaattg
     4081 gaccgtgtca agctgagctt ttccatgagc ctcctgagcc gctttgtgga tgggctgacc
     4141 ttcaaggtga actttacctt caaccgccag ccgctgcgag tccagcaccg tgccctggag
     4201 ctgacagggc gctggctgct gtggcccatg ctctttcctg tggcacctcg ggacgtcccg
     4261 ctgctgccct cagatgtgaa actcaagctg tacgaccgga gtctggagtc aaacccagag
     4321 cagctgcagg ccatgaggca cattgttacg ggcaccaccc gtccagcccc ctacatcatc
     4381 tttgggcctc caggcaccgg caagactgtc acgttagtgg aggcaattaa gcaggtggtg
     4441 aagcacttgc ccaaagccca catcttggcc tgcgctccat ccaactcagg ggctgaccta
     4501 ctctgtcaaa ggctccgggt ccaccttcct agctccatct accgcctcct ggcccccagc
     4561 agggacatcc gcatggtacc tgaggacatc aagccctgct gcaactggga cgcaaagaag
     4621 ggggagtatg tatttcccgc caagaagaag ctgcaggaat accgggtctt aattaccacc
     4681 ctcatcactg ccggcaggtt ggtctcggcc cagtttccca ttgatcactt cacacacatc
     4741 ttcatcgatg aggctggcca ctgcatggag cctgagagtc tggtagctat agcagggctg
     4801 atggaagtaa aggaaacagg tgatccagga gggcagctgg tgctggcagg agaccctcgg
     4861 cagctggggc ctgtgctgcg ttccccactg acccagaagc atggactggg atactcactg
     4921 ctggagcggc tgctcaccta caactccctg tacaagaagg gccctgatgg ctatgacccc
     4981 cagttcataa ccaagctgct ccgcaactac aggtctcatc ccaccatcct ggacattcct
     5041 aaccagctct attatgaagg ggagctgcag gcctgtgctg atgtcgtgga tcgagaacgc
     5101 ttctgccgct gggcgggcct acctcgacag ggctttccca tcatctttca cggcgtaatg
     5161 ggcaaagatg agcgtgaagg caacagccca tccttcttca accctgaaga ggctgccaca
     5221 gtgacttcct acctgaagct gctcctggcc ccctcctcca agaagggcaa agctcgcctg
     5281 agccctcgaa gtgtgggcgt catctccccg taccggaaac aggtggagaa aatccgttac
     5341 tgcatcacca aacttgacag ggagcttcga ggactggatg acatcaagga cttgaaggtg
     5401 ggttcagtag aagaattcca aggccaagaa cgaagcgtca tcctcatctc caccgtgcga
     5461 agcagccaga gctttgtgca gctggatctg gactttaatc tgggtttcct taagaacccc
     5521 aagaggttca atgtagctgt gacccgggcc aaggccctgc tcatcatcgt ggggaacccc
     5581 cttctcctgg gccatgaccc tgactggaaa gtattcctgg agttctgtaa agaaaacgga
     5641 gggtataccg ggtgtccctt ccctgccaaa ctggacctgc aacagggaca gaatttactg
     5701 caaggtctga gcaagctcag cccctctacc tcagggcccc acagccatga ctacctcccc
     5761 caggagcggg agggtgaagg gggcctgtct ctgcaagtgg agccagagtg gaggaatgag
     5821 ctctgagcgg ccgcggatcc cgcccctctc cctccccccc ccctaacgtt actggccgaa
     5881 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
     5941 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
     6001 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
     6061 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
     6121 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
     6181 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
     6241 tcacctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
     6301 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaacg
     6361 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg
     6421 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc
     6481 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     6541 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg
     6601 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg
     6661 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     6721 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg
     6781 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc
     6841 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc
     6901 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg
     6961 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca
     7021 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg
     7081 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtgaacg cgtctggaac
     7141 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     7201 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     7261 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     7321 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     7381 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     7441 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     7501 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     7561 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     7621 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     7681 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattaat
     7741 tctgcagtcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca
     7801 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac
     7861 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     7921 aagaaaagag gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc
     7981 tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag
     8041 gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata
     8101 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg
     8161 ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc
     8221 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct
     8281 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt
     8341 ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
     8401 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     8461 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     8521 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     8581 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     8641 ccttgacatt gctagcgttt accgtcgacc tctagctaga gcttggcgta atcatggtca
     8701 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     8761 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     8821 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     8881 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     8941 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     9001 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     9061 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     9121 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     9181 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     9241 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     9301 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     9361 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     9421 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     9481 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     9541 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     9601 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     9661 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     9721 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     9781 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     9841 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     9901 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     9961 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
    10021 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    10081 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    10141 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    10201 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    10261 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    10321 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    10381 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    10441 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    10501 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    10561 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    10621 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    10681 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    10741 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    10801 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
    10861 tcgggagatc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
    10921 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
    10981 caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa
    11041 acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac
    11101 ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac
    11161 aaacttagta gt
//
