LOCUS V021370 8676 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V021370 VERSION V021370 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2815..3327 /label="SAT1(NM_002970)" /note="SAT1(NM_002970)" /gene="SAT1" misc_feature 3347..3919 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" /label="IRES" CDS 3921..4631 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /transl_table=1 /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK*" /label="mCherry" misc_feature 4645..5233 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 5440..6073 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(6201..6217) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6225..6241 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6249..6279) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6294..6315 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6603..7191) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7362..8222) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8223..8327) /gene="bla" /label="AmpR promoter" polyA_signal 8375..8509 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatggct 2821 aaattcgtga tccgcccagc cactgccgcc gactgcagtg acatactgcg gctgatcaag 2881 gagctggcta aatatgaata catggaagaa caagtaatct taactgaaaa agatctgcta 2941 gaagatggtt ttggagagca ccccttttac cactgcctgg ttgcagaagt gccgaaagag 3001 cactggactc cggaaggaca cagcattgtt ggttttgcca tgtactattt tacctatgac 3061 ccgtggattg gcaagttatt gtatcttgag gacttcttcg tgatgagtga ttatagaggc 3121 tttggcatag gatcagaaat tctgaagaat ctaagccagg ttgcaatgag gtgtcgctgc 3181 agcagcatgc acttcttggt agcagaatgg aatgaaccat ccatcaactt ctataaaaga 3241 agaggtgctt ctgatctgtc cagtgaagag ggttggagac tgttcaagat cgacaaggag 3301 tacttgctaa aaatggcaac agaggagtga gcggccgcgg atcccgcccc tctccctccc 3361 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata 3421 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg 3481 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt 3541 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag 3601 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc 3661 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga 3721 tagttgtgga aagagtcaaa tggctcacct caagcgtatt caacaagggg ctgaaggatg 3781 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat 3841 gtgtttagtc gaggttaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct 3901 ttgaaaaaca cgatgataat atggtgagca agggcgagga ggataacatg gccatcatca 3961 aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga 4021 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga 4081 ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct 4141 ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg 4201 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc 4261 aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact 4321 tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc 4381 ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg 4441 acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc 4501 tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca 4561 ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc 4621 tgtacaagtg aacgcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg 4681 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct 4741 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg 4801 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact 4861 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc 4921 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc 4981 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag 5041 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc 5101 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg 5161 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg 5221 gccgcctccc cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca 5281 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg 5341 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag 5401 ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc 5461 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt 5521 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca 5581 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct 5641 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga 5701 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca 5761 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc 5821 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt 5881 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa 5941 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt 6001 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg 6061 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag 6121 aaatgaatat cagagagtga gaggccttga cattgctagc gtttaccgtc gacctctagc 6181 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 6241 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 6301 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 6361 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 6421 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 6481 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 6541 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 6601 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 6661 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 6721 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 6781 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 6841 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 6901 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 6961 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 7021 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt 7081 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 7141 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 7201 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 7261 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 7321 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 7381 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 7441 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 7501 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 7561 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 7621 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 7681 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 7741 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 7801 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 7861 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 7921 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 7981 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 8041 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 8101 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 8161 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 8221 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 8281 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 8341 aaagtgccac ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg 8401 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 8461 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac 8521 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta 8581 cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa 8641 ttgtatttgt taaatatgta ctacaaactt agtagt //