LOCUS V021282 8622 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V021282 VERSION V021282 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2815..3273 /label="UBE2L6(NM_004223)" /note="UBE2L6(NM_004223)" /gene="UBE2L6" misc_feature 3293..3865 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" /label="IRES" CDS 3867..4577 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /transl_table=1 /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK*" /label="mCherry" misc_feature 4591..5179 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 5386..6019 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(6147..6163) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6171..6187 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6195..6225) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6240..6261 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6549..7137) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7308..8168) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8169..8273) /gene="bla" /label="AmpR promoter" polyA_signal 8321..8455 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatgatg 2821 gcgagcatgc gagtggtgaa ggagctggag gatcttcaga agaagcctcc cccatacctg 2881 cggaacctgt ccagcgatga tgccaatgtc ctggtgtggc acgctctcct cctacccgac 2941 caacctccct accacctgaa agccttcaac ctgcgcatca gcttcccgcc ggagtatccg 3001 ttcaagcctc ccatgatcaa attcacaacc aagatctacc accccaacgt ggacgagaac 3061 ggacagattt gcctgcccat catcagcagt gagaactgga agccttgcac caagacttgc 3121 caagtcctgg aggccctcaa tgtgctggtg aatagaccga atatcaggga gcccctgcgg 3181 atggacctcg ctgacctgct gacacagaat ccggagctgt tcagaaagaa tgccgaagag 3241 ttcaccctcc gattcggagt ggaccggccc tcctaagcgg ccgcggatcc cgcccctctc 3301 cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg 3361 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg 3421 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag 3481 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt 3541 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc 3601 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga 3661 gttggatagt tgtggaaaga gtcaaatggc tcacctcaag cgtattcaac aaggggctga 3721 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct 3781 ttacatgtgt ttagtcgagg ttaaaaaacg tctaggcccc ccgaaccacg gggacgtggt 3841 tttcctttga aaaacacgat gataatatgg tgagcaaggg cgaggaggat aacatggcca 3901 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt 3961 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga 4021 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt 4081 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct 4141 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg 4201 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca 4261 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct 4321 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc 4381 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg 4441 tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg 4501 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg 4561 acgagctgta caagtgaacg cgtctggaac aatcaacctc tggattacaa aatttgtgaa 4621 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 4681 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 4741 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 4801 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 4861 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 4921 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 4981 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg 5041 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 5101 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc 5161 ctttgggccg cctccccgcc tggaattaat tctgcagtcg agacctagaa aaacatggag 5221 caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag 5281 aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca 5341 aggcagctgt agatcttagc cactttttaa aagaaaagag gggactggaa gggctaattc 5401 actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc 5461 ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt 5521 gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca 5581 ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag 5641 agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt 5701 acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag 5761 ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag 5821 ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct 5881 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta 5941 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca 6001 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt 6061 gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gctagcgttt accgtcgacc 6121 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg 6181 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa 6241 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac 6301 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt 6361 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 6421 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 6481 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 6541 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 6601 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 6661 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 6721 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 6781 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 6841 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 6901 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 6961 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag 7021 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 7081 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 7141 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 7201 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 7261 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 7321 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 7381 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 7441 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 7501 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 7561 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 7621 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 7681 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 7741 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 7801 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 7861 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 7921 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 7981 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 8041 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 8101 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 8161 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 8221 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 8281 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc aacttgttta ttgcagctta 8341 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 8401 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg 8461 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc 8521 caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta 8581 aagaaattgt atttgttaaa tatgtactac aaacttagta gt //