LOCUS V031390 10025 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V031390 VERSION V031390 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2815..4551 /label="YTHDF2(NM_001173128)" /note="YTHDF2(NM_001173128)" /gene="YTHDF2" CDS 4561..4587 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" misc_feature 4599..5185 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" /label="IRES2" CDS 5186..5980 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" misc_feature 5994..6582 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6789..7422 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(7550..7566) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7574..7590 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7598..7628) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7643..7664 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7952..8540) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8711..9571) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9572..9676) /gene="bla" /label="AmpR promoter" polyA_signal 9724..9858 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatgtcg 2821 gccagcagcc tcttggagca gagaccaaaa ggtcaaggaa acaaagtaca aaatggatct 2881 gtacatcaaa aggatggatt aaacgatgat gattttgaac cttacttgag tccacaggca 2941 aggcccaata atgcatatac tgccatgtca gattcctact tacccagtta ctacagtccc 3001 tccattggct tctcctattc tttgggtgaa gctgcttggt ctacgggggg tgacacagcc 3061 atgccctact taacttctta tggacagctg agcaacggag agccccactt cctaccagat 3121 gcaatgtttg ggcaaccagg agccctaggt agcactccat ttcttggtca gcatggtttt 3181 aatttctttc ccagtgggat tgacttctca gcatggggaa ataacagttc tcagggacag 3241 tctactcaga gctctggata tagtagcaat tatgcttatg cacctagctc cttaggtgga 3301 gccatgattg atggacagtc agcttttgcc aatgagaccc tcaataaggc tcctggcatg 3361 aatactatag accaagggat ggcagcactg aagttgggta gcacagaagt tgcaagcaat 3421 gttccaaaag ttgtaggttc tgctgttggt agcgggtcca ttactagtaa catcgtggct 3481 tccaatagtt tgcctccagc caccattgct cctccaaaac cagcatcttg ggctgatatt 3541 gctagcaagc ctgcaaaaca gcaacctaaa ctgaagacca agaatggcat tgcagggtca 3601 agtcttccgc cacccccgat aaagcataac atggatattg gaacttggga taacaagggt 3661 cccgttgcaa aagccccctc acaggctttg gttcagaata taggtcagcc aacccagggg 3721 tctcctcagc ctgtaggtca gcaggctaac aatagcccac cagtggctca ggcatcagta 3781 gggcaacaga cacagccatt gcctccacct ccaccacagc ctgcccagct ttcagtccag 3841 caacaggcag ctcagccaac ccgctgggta gcacctcgga accgtggcag tgggttcggt 3901 cataatgggg tggatggtaa tggagtagga cagtctcagg ctggttctgg atctactcct 3961 tcagaacccc acccagtgtt ggagaagctt cggtccatta ataactataa ccccaaagat 4021 tttgactgga atctgaaaca tggccgggtt ttcatcatta agagctactc tgaggacgat 4081 attcaccgtt ccattaagta taatatttgg tgcagcacag agcatggtaa caagagactg 4141 gatgctgctt atcgttccat gaacgggaaa ggccccgttt acttactttt cagtgtcaac 4201 ggcagtggac acttctgtgg cgtggcagaa atgaaatctg ctgtggacta caacacatgt 4261 gcaggtgtgt ggtcccagga caaatggaag ggtcgttttg atgtcaggtg gatttttgtg 4321 aaggacgttc ccaatagcca actgcgacac attcgcctag agaacaacga gaataaacca 4381 gtgaccaact ctagggacac tcaggaagtg cctctggaaa aggctaagca ggtgttgaaa 4441 attatagcca gctacaagca caccacttcc atttttgatg acttctcaca ctatgagaaa 4501 cgccaagagg aagaagaaag tgttaaaaag gaacgtcaag gtcgtgggaa aggcggtagt 4561 tatccttatg acgtgcctga ctatgcctga ggatcccgcc cctctccctc ccccccccct 4621 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt 4681 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg 4741 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc 4801 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt 4861 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta 4921 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg 4981 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag 5041 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag 5101 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa 5161 acacgatgat aagcttgcca caaccatggc tgaacaagat ggattgcacg caggttctcc 5221 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc 5281 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga 5341 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac 5401 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct 5461 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa 5521 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc 5581 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct 5641 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc 5701 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg 5761 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct 5821 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct 5881 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca 5941 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga acgcgtctgg aacaatcaac 6001 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta 6061 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt 6121 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg 6181 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg 6241 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca 6301 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca 6361 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg 6421 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag 6481 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc 6541 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt aattctgcag 6601 tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga 6661 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt 6721 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa 6781 gaggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat 6841 ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc caggggtcag 6901 atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga 6961 agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga 7021 tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt 7081 ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg 7141 actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc 7201 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc 7261 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa 7321 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga 7381 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc 7441 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggccttgac 7501 attgctagcg tttaccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt 7561 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 7621 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 7681 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 7741 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 7801 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 7861 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 7921 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 7981 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 8041 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 8101 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 8161 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 8221 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 8281 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 8341 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 8401 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 8461 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 8521 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 8581 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 8641 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 8701 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 8761 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 8821 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 8881 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 8941 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 9001 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 9061 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 9121 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 9181 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 9241 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 9301 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 9361 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 9421 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 9481 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 9541 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 9601 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 9661 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag 9721 atcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 9781 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 9841 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc 9901 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat 9961 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta 10021 gtagt //