LOCUS       V040383                10014 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040383
VERSION     V040383
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2843..4060
                     /label="ANGPTL4(NM_139314)"
                     /note="ANGPTL4(NM_139314)"
                     /gene="ANGPTL4"
     CDS             4112..4822
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK*"
                     /label="mCherry"
     promoter        4849..5348
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             5369..5968
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5982..6570
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6777..7410
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(7539..7555)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7563..7579
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7587..7617)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7632..7653
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7941..8529)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8700..9560)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9561..9665)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    9713..9847
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattcgcca ccatgagcgg tgctccgacg gccggggcag ccctgatgct
     2881 ctgcgccgcc accgccgtgc tactgagcgc tcagggcgga cccgtgcagt ccaagtcgcc
     2941 gcgctttgcg tcctgggacg agatgaatgt cctggcgcac ggactcctgc agctcggcca
     3001 ggggctgcgc gaacacgcgg agcgcacccg cagtcagctg agcgcgctgg agcggcgcct
     3061 gagcgcgtgc gggtccgcct gtcagggaac cgaggggtcc accgacctcc cgttagcccc
     3121 tgagagccgg gtggaccctg aggtccttca cagcctgcag acacaactca aggctcagaa
     3181 cagcaggatc cagcaactct tccacaaggt ggcccagcag cagcggcacc tggagaagca
     3241 gcacctgcga attcagcatc tgcaaagcca gtttggcctc ctggaccaca agcacctaga
     3301 ccatgaggtg gccaagcctg cccgaagaaa gaggctgccc gagatggccc agccagttga
     3361 cccggctcac aatgtcagcc gcctgcaccg gctgcccagg gattgccagg agctgttcca
     3421 ggttggggag aggcagagtg gactatttga aatccagcct caggggtctc cgccattttt
     3481 ggtgaactgc aagatgacct cagatggagg ctggacagta attcagaggc gccacgatgg
     3541 ctcagtggac ttcaaccggc cctgggaagc ctacaaggcg gggtttgggg atccccacgg
     3601 cgagttctgg ctgggtctgg agaaggtgca tagcatcacg ggggaccgca acagccgcct
     3661 ggccgtgcag ctgcgggact gggatggcaa cgccgagttg ctgcagttct ccgtgcacct
     3721 gggtggcgag gacacggcct atagcctgca gctcactgca cccgtggccg gccagctggg
     3781 cgccaccacc gtcccaccca gcggcctctc cgtacccttc tccacttggg accaggatca
     3841 cgacctccgc agggacaaga actgcgccaa gagcctctct ggaggctggt ggtttggcac
     3901 ctgcagccat tccaacctca acggccagta cttccgctcc atcccacagc agcggcagaa
     3961 gcttaagaag ggaatcttct ggaagacctg gcggggccgc tactacccgc tgcaggccac
     4021 caccatgttg atccagccca tggcagcaga ggcagcctcc ggtggaggcg gatcaggagg
     4081 cggtggatct gcggatccac cggtcgccac catggtgagc aagggcgagg aggataacat
     4141 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
     4201 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     4261 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
     4321 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
     4381 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     4441 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
     4501 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     4561 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
     4621 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
     4681 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
     4741 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
     4801 catggacgag ctgtacaagt agcggccgcg actctagata attctaccgg gtaggggagg
     4861 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct
     4921 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc
     4981 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc
     5041 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc
     5101 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc
     5161 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg
     5221 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg
     5281 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg
     5341 gcctttcgac ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc
     5401 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     5461 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     5521 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     5581 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     5641 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     5701 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     5761 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     5821 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     5881 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     5941 gtgcatgacc cgcaagcccg gtgcctgacc gcgtctggaa caatcaacct ctggattaca
     6001 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     6061 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     6121 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     6181 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     6241 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     6301 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     6361 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     6421 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     6481 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     6541 gtcggatctc cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga
     6601 aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct
     6661 agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc
     6721 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga
     6781 agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca
     6841 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
     6901 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
     6961 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
     7021 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
     7081 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
     7141 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
     7201 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct
     7261 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     7321 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     7381 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     7441 atttataact tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt
     7501 ttaccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     7561 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     7621 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     7681 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     7741 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     7801 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     7861 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     7921 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     7981 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     8041 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     8101 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     8161 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     8221 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     8281 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     8341 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     8401 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     8461 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     8521 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     8581 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     8641 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     8701 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     8761 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     8821 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     8881 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     8941 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     9001 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     9061 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     9121 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     9181 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     9241 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     9301 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     9361 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     9421 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     9481 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     9541 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     9601 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     9661 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt
     9721 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     9781 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     9841 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
     9901 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
     9961 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagt
//
