LOCUS V041005 11272 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V041005 VERSION V041005 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2843..5968 /label="CORIN(NM_006587)" /note="CORIN(NM_006587)" /gene="CORIN" CDS 5975..6040 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 6060..6559 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 6580..7179 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 7193..7781 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 7988..8621 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(8750..8766) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 8774..8790 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(8798..8828) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 8843..8864 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(9152..9740) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(9911..10771) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(10772..10876) /gene="bla" /label="AmpR promoter" polyA_signal 10924..11058 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcgcca ccatgaaaca gtctcctgcc ctcgctccgg aagagcgctg 2881 ccgcagagcc gggtccccaa agccggtctt gagagctgat gacaataaca tgggcaatgg 2941 ctgctctcag aagctggcga ctgctaacct cctccggttc ctattgctgg tcctgattcc 3001 atgtatctgt gctctcgttc tcttgctggt gatcctgctt tcctatgttg gaacattaca 3061 aaaggtctat tttaaatcaa atgggagtga acctttggtc actgatggtg aaatccaagg 3121 gtccgatgtt attcttacaa atacaattta taaccagagc actgtggtgt ctactgcaca 3181 tcccgaccaa cacgttccag cctggactac ggatgcttct ctcccagggg accaaagtca 3241 caggaataca agtgcctgta tgaacatcac ccacagccag tgtcagatgc tgccctacca 3301 cgccacgctg acacctctcc tctcagttgt cagaaacatg gaaatggaaa agttcctcaa 3361 gtttttcaca tatctccatc gcctcagttg ctatcaacat atcatgctgt ttggctgtac 3421 cctcgccttc cctgagtgca tcattgatgg cgatgacagt catggactcc tgccctgtag 3481 gtccttctgt gaggctgcaa aagaaggctg tgaatcagtc ctggggatgg tgaattactc 3541 ctggccggat ttcctcagat gctcccagtt tagaaaccaa actgaaagca gcaatgtcag 3601 cagaatttgc ttctcacctc agcaggaaaa cggaaagcaa ttgctctgtg gaaggggtga 3661 gaactttctg tgtgccagtg gaatctgcat ccccgggaaa ctgcaatgta atggctacaa 3721 cgactgtgac gactggagtg acgaggctca ttgcaactgc agcgagaatc tgtttcactg 3781 tcacacaggc aagtgcctta attacagcct tgtgtgtgat ggatatgatg actgtgggga 3841 tttgagtgat gagcaaaact gtgattgcaa tcccacaaca gagcatcgct gcggggacgg 3901 gcgctgcatc gccatggagt gggtgtgtga tggtgaccac gactgtgtgg ataagtctga 3961 cgaggtcaac tgctcctgtc acagccaggg tctggtggaa tgcagaaatg gacaatgtat 4021 ccccagcacg tttcaatgtg atggtgacga ggactgcaag gatgggagtg atgaggagaa 4081 ctgcagcgtc attcagactt catgtcaaga aggagaccaa agatgcctct acaatccctg 4141 ccttgattca tgtggtggta gctctctctg tgacccgaac aacagtctga ataactgtag 4201 tcaatgtgaa ccaattacat tggaactctg catgaatttg ccctacaaca gtacaagtta 4261 tccaaattat tttggccaca ggactcaaaa ggaagcatcc atcagctggg agtcttctct 4321 tttccctgca cttgttcaaa ccaactgtta taaatacctc atgttctttt cttgcaccat 4381 tttggtacca aaatgtgatg tgaatacagg cgagcatatc cctccttgca gggcattgtg 4441 tgaacactct aaagaacgct gtgagtctgt tcttgggatt gtgggcctac agtggcctga 4501 agacacagat tgcagtcaat ttccagagga aaattcagac aatcaaacct gcctgatgcc 4561 tgatgaatat gtggaagaat gctcacctag tcatttcaag tgccgctcag gacagtgtgt 4621 tctggcttcc agaagatgtg atggccaggc cgactgtgac gatgacagtg atgaggaaaa 4681 ctgtggttgt aaagagagag atctttggga atgtccatcc aataaacaat gtttgaagca 4741 cacagtgatc tgcgatgggt tcccagactg ccctgattac atggacgaga aaaactgctc 4801 attttgccaa gatgatgagc tggaatgtgc aaaccatgcg tgtgtgtcac gtgacctgtg 4861 gtgtgatggt gaagccgact gctcagacag ttcagatgaa tgggactgtg tgaccctctc 4921 tataaatgtg aactcctctt cctttctgat ggttcacaga gctgccacag aacaccatgt 4981 gtgtgcagat ggctggcagg agatattgag tcagctggcc tgcaagcaga tgggtttagg 5041 agaaccatct gtgaccaaat tgatacagga acaggagaaa gagccgcggt ggctgacatt 5101 acactccaac tgggagagcc tcaatgggac cactttacat gaacttctag taaatgggca 5161 gtcttgtgag agcagaagta aaatttctct tctgtgtact aaacaagact gtgggcgccg 5221 ccctgctgcc cgaatgaaca aaaggatcct tggaggtcgg acgagtcgcc ctggaaggtg 5281 gccatggcag tgttctctgc agagtgaacc cagtggacat atctgtggct gtgtcctcat 5341 tgccaagaag tgggttctga cagttgccca ctgcttcgag gggagagaga atgctgcagt 5401 ttggaaagtg gtgcttggca tcaacaatct agaccatcca tcagtgttca tgcagacacg 5461 ctttgtgaag accatcatcc tgcatccccg ctacagtcga gcagtggtgg actatgacat 5521 cagcatcgtt gagctgagtg aagacatcag tgagactggc tacgtccggc ctgtctgctt 5581 gcccaacccg gagcagtggc tagagcctga cacgtactgc tatatcacag gctggggcca 5641 catgggcaat aaaatgccat ttaagctgca agagggagag gtccgcatta tttctctgga 5701 acattgtcag tcctactttg acatgaagac catcaccact cggatgatat gtgctggcta 5761 tgagtctggc acagttgatt catgcatggg tgacagcggt gggcctcttg tttgtgagaa 5821 gcctggagga cggtggacat tatttggatt aacttcatgg ggctccgtct gcttttccaa 5881 agtcctgggg cctggcgttt atagtaatgt gtcatatttc gtcgaatgga ttaaaagaca 5941 gatttacatc cagacctttc tcctaaacgg atccgactac aaagaccatg acggtgatta 6001 taaagatcat gacatcgact acaaggatga cgatgacaag tagtctagat aattctaccg 6061 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg 6121 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg 6181 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag 6241 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg 6301 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg 6361 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg 6421 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc 6481 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc 6541 ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta caagcccacg 6601 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc 6661 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc 6721 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg 6781 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg 6841 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa 6901 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc 6961 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga 7021 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac 7081 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga 7141 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac cgcgtctgga acaatcaacc 7201 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac 7261 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt 7321 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt 7381 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg 7441 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac 7501 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac 7561 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt 7621 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc 7681 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg 7741 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta attctgcagt 7801 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat 7861 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta 7921 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag 7981 aggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc 8041 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga 8101 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa 8161 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat 8221 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg 8281 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga 8341 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc 8401 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca 8461 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag 8521 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag 8581 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct 8641 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca 8701 ttgctagcgt tttaccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt 8761 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 8821 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 8881 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 8941 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 9001 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 9061 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 9121 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 9181 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 9241 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 9301 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 9361 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 9421 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 9481 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 9541 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 9601 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 9661 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 9721 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 9781 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 9841 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 9901 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 9961 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 10021 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 10081 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 10141 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 10201 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 10261 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 10321 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 10381 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 10441 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 10501 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 10561 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 10621 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 10681 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 10741 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 10801 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 10861 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag 10921 atcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 10981 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 11041 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc 11101 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat 11161 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta 11221 gtagttttta aagaaattgt atttgttaaa tatgtactac aaacttagta gt //