LOCUS       V032291                11365 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032291
VERSION     V032291
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2843..6100
                     /label="Cp(NM_001409646)"
                     /note="Cp(NM_001409646)"
                     /gene="Cp"
     CDS             6107..6133
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        6153..6652
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             6673..7272
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7286..7874
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8081..8714
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8843..8859)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8867..8883
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8891..8921)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8936..8957
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9245..9833)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10004..10864)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10865..10969)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    11017..11151
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattcgcca ccatgaagtt tttgctgctt agcacattta tatttttgta
     2881 tagttcctta gccttggcaa gagataagca ttatttcatt ggaattactg aagcagtctg
     2941 ggactatgct tctggcactg aagaaaagaa acttatttca gttgacacgg aacagtccaa
     3001 tttctatctt caaaatggtc cagatcgtat tggaagaaaa tataagaagg ccctttattt
     3061 tgagtacaca gatggcacct ttagtaagac tatagacaaa ccagcctggc tagggttttt
     3121 aggccctgtc atcaaagctg aagttgaaga taaagtttat gttcacttaa agaaccttgc
     3181 ctctaggatc tacacttttc atgcacatgg ggtaacgtac accaaggagt atgagggagc
     3241 cgtctaccct gacaacacca ctgattttca acgggctgat gacaaagtgc ttcccggaca
     3301 acagtatgtg tatgtgctgc atgccaatga gccaagtcct ggagagggag acagcaattg
     3361 tgtgaccagg atttaccact cccatgttga tgctccaaaa gatattgcat caggactcat
     3421 aggacctcta atactctgta aaaaaggttc tctatataag gaaaaagaga aaaatattga
     3481 ccaagaattt gtactaatgt tctctgtggt ggatgaaaat ctcagctggt atctggaaga
     3541 taacatcaaa accttctgct ctgaacccga gaaagttgat aaagacaatg aagacttcca
     3601 ggaaagcaac aggatgtact ctataaatgg atatacattt ggaagcctcc cagggctctc
     3661 gatgtgtgca gcagacagag tgaagtggta cctttttggt atgggtaatg aagttgatgt
     3721 gcattcagct ttctttcatg gccaagccct gaccagcagg aactatcaaa ccgatataat
     3781 caacctgttc cctgccaccc taattgatgc ttatatggtg gcccagaatc ctggagtctg
     3841 gatgctcagt tgccagaacc taaaccatct gaaagctggg ttgcaggcct ttttccaggt
     3901 tcgtgactgt aacaagccct cgccagagga taatatccaa gataggcatg tgagacacta
     3961 ttacattgct gctgaggagg tcatctggaa ttatgctcct tctgggacgg acatcttcac
     4021 tggagagaat ttaacagctc tggaaagtga ttcaagggta ttttttgagc aaggtgccac
     4081 aagaattggt ggctcttata aaaaaatggc atatcgtgag tacacagatg gttccttcac
     4141 aaaccgaaaa caaagaggcc ctgatgagga acatcttgga atcctaggtc ctgtcatttg
     4201 ggcagaagta ggagacacca ttaaagtcac ctttcataac aaaggacagc atcctctcag
     4261 cattcagcca atgggagtaa gtttcactgc agaaaatgag ggaacatact atggcccacc
     4321 aggtcgctcc tcacagcaag cagcctccca tgtggctccc aaagaaacct ttacatacga
     4381 atggactgtc cccaaagaaa tgggacccac ttatgcagat cctgtgtgcc tatctaagat
     4441 gtactactct ggcgttgacc ccaccaaaga tatatttact gggcttattg ggccaatgaa
     4501 aatatgcaag aaaggcagct tacttgctga tgggagacag aaagatgtag acaaagagtt
     4561 ctacttgttt cccacagtgt ttgatgagaa tgagagttta ctcttagatg ataatatcag
     4621 gatgttcaca actgcacctg atcaagtgga taaggaagat gaagactttc aggagtctaa
     4681 taagatgcac tccatgaatg ggttcatgta tggcaatcag cctggcctca atatgtgtct
     4741 aggagaatcc atcgtgtggt atttgttcag cgctggaaat gaggctgatg tgcatgggat
     4801 atacttttca ggaaatactt atctgtctaa aggagaaaga agagacactg caaacctatt
     4861 ccctcataaa agtctcaccc ttctcatgaa ccctgacaca aaagggactt ttgatgttga
     4921 gtgccttaca acggatcact acacaggtgg catgaagcaa aaatacactg tgaaccagtg
     4981 ccagcggcag tttgaagatt tcactgtcta ccttggagaa aggacctact atgtggcagc
     5041 cgtagaggtg gaatgggatt actcaccaag cagggcctgg gaaaaggagc tgcatcattt
     5101 gcaagagcaa aatgtttcaa atgtattttt ggataaagaa gagtttttca taggctcaaa
     5161 gtacaagaag gttgtgtatc gccagtttac tgacagctca ttcagagaac aggtgaagag
     5221 acgagccgaa gacgagcact tgggcatcct tgggccacca attcatgcaa atgttggaga
     5281 caaagttaaa gttgtcttta aaaatatggc aaccaggcca tattcaatac atgcccatgg
     5341 ggtgaaaaca gagagttcta cagttgttcc aacgttacca ggtgaagttc gaacttatac
     5401 atggcaaatt ccagaaagat caggggctgg aagagaggat tcagcttgta tcccatgggc
     5461 ttattactca actgtggatc gagttaagga tctctatagt gggctaatag gcccattgat
     5521 tgtttgtcgg aagtcttatg tgaaagtatt cagtcctaaa aagaaaatgg agtttttcct
     5581 tctgtttcta gtatttgatg agaatgaatc ttggtactta gatgataaca tcaaaacata
     5641 ctctgaacac cctgagaaag taaacaaaga caacgaggaa ttcctagaaa gcaataaaat
     5701 gcatgctatt aatgggaaaa tgtttggaaa cctacaaggc ctcacaatgc acgtgaaaga
     5761 tgaagtcaac tggtatgtga tgggaatggg caatgaaata gacctgcaca ctgtacactt
     5821 ccacggccac agcttccaat acaagcacag gggagtatac agttctgatg tctttgacct
     5881 tttccctgga acataccaaa ccctagaaat gtttccccaa acacctggaa cctggttact
     5941 ccactgccac gtgactgacc atgtccatgc tgggatggca actacctaca ctgttttacc
     6001 agtagaacaa gtatcatctc agagttacag gatgacctgg aacatcctct atacactact
     6061 aatcagcatc attattttat tccaaatgtc taccaaggag ggatcctacc catacgacgt
     6121 accagattac gcttagtcta gataattcta ccgggtaggg gaggcgcttt tcccaaggca
     6181 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg
     6241 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc
     6301 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc
     6361 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag
     6421 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
     6481 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg
     6541 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
     6601 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag
     6661 cccaagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     6721 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     6781 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     6841 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     6901 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     6961 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     7021 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     7081 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     7141 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     7201 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     7261 cccggtgcct gaccgcgtct ggaacaatca acctctggat tacaaaattt gtgaaagatt
     7321 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     7381 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     7441 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     7501 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     7561 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     7621 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     7681 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     7741 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     7801 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     7861 ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca tggagcaatc
     7921 acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag
     7981 gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca
     8041 gctgtagatc ttagccactt tttaaaagaa aagaggggac tggaagggct aattcactcc
     8101 caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
     8161 tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac
     8221 aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc
     8281 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg
     8341 aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc
     8401 aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg
     8461 cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct
     8521 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     8581 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     8641 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     8701 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     8761 gaaatgaata tcagagagtg agaggccttg acattgctag cgttttaccg tcgacctcta
     8821 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     8881 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     8941 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     9001 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     9061 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     9121 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     9181 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     9241 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     9301 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     9361 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     9421 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     9481 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     9541 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     9601 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     9661 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     9721 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     9781 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     9841 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     9901 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     9961 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
    10021 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
    10081 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
    10141 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
    10201 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
    10261 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
    10321 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
    10381 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
    10441 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
    10501 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
    10561 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
    10621 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
    10681 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
    10741 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    10801 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    10861 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    10921 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    10981 gaaaagtgcc acctgacgtc gacggatcgg gagatcaact tgtttattgc agcttataat
    11041 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
    11101 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata
    11161 actcaagcta accaaaatca tcccaaactt cccaccccat accctattac cactgccaat
    11221 tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga
    11281 aattgtattt gttaaatatg tactacaaac ttagtagttt ttaaagaaat tgtatttgtt
    11341 aaatatgtac tacaaactta gtagt
//
