LOCUS       V021109                11270 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021109
VERSION     V021109
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2826..6005
                     /label="DNA2(NM_001080449)"
                     /note="DNA2(NM_001080449)"
                     /gene="DNA2"
     CDS             6012..6038
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        6058..6557
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             6578..7177
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7191..7779
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7986..8619
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8748..8764)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8772..8788
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8796..8826)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8841..8862
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9150..9738)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9909..10769)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10770..10874)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    10922..11056
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ccaccatgga gcagctgaac gaactggagc tgctgatgga gaagagtttt tgggaggagg
     2881 cggagctgcc ggcggagcta tttcagaaga aagtggtagc ttcctttcca agaacagttc
     2941 tgagcacagg aatggataac cggtacctgg tgttggcagt caatactgta cagaacaaag
     3001 agggaaactg tgaaaagcgc ctggtcatca ctgcttcaca gtcactagaa aataaagaac
     3061 tatgcatcct taggaatgac tggtgttctg ttccagtaga gccaggagat atcattcatt
     3121 tggagggaga ctgcacatct gacacttgga taatagataa agattttgga tatttgattc
     3181 tgtatccaga catgctgatt tctggcacca gcatagccag tagtattcga tgtatgagaa
     3241 gagctgtcct gagtgaaact tttaggagct ctgatccagc cacacgccaa atgctaattg
     3301 gtacggttct ccatgaggtg tttcaaaaag ccataaataa tagctttgcc ccagaaaagc
     3361 tacaagaact tgcttttcaa acaattcaag aaataagaca tttgaaggaa atgtaccgct
     3421 taaatctaag tcaagatgaa ataaaacaag aagtagagga ctatcttcct tcgttttgta
     3481 aatgggcagg agatttcatg cataaaaaca cttcgactga cttccctcag atgcagctct
     3541 ctctgccaag tgataatagt aaggataatt caacatgtaa cattgaagtc gtgaaaccaa
     3601 tggatattga agaaagcatt tggtccccta ggtttggatt gaaaggcaaa atagatgtta
     3661 cagttggtgt gaaaatacat cgagggtata aaacaaaata caagataatg ccgctggaac
     3721 ttaaaactgg caaagaatca aattctattg aacaccgtag tcaggttgtt ctgtacactc
     3781 tactaagcca agagagaaga gctgatccag aggctggctt gcttctctac ctcaagactg
     3841 gtcagatgta ccctgtgcct gccaaccatc tagataaaag agaattatta aagctaagaa
     3901 accagatggc attctcattg tttcaccgta ttagcaaatc tgctactaga cagaagacac
     3961 agcttgcttc tttgccacaa ataattgagg aagagaaaac ttgtaaatat tgttcacaaa
     4021 ttggcaattg tgctctttat agcagagcag ttgaacaaca gatggattgt agttcagtcc
     4081 caattgtgat gctgcccaaa atagaagaag aaacccagca tctgaagcaa acacacttag
     4141 aatatttcag cctttggtgt ctaatgttaa ccctggagtc acaatcgaag gataataaaa
     4201 agaatcacca aaatatctgg ctaatgcctg cttcggaaat ggagaagagt ggcagttgca
     4261 ttggaaacct gattagaatg gaacatgtaa agatagtttg tgatgggcaa tatttacata
     4321 atttccaatg taaacatggt gccatacctg tcacaaatct aatggcaggt gacagagtta
     4381 ttgtaagtgg agaagaaagg tcactgtttg ctttgtctag aggatatgtg aaggagatta
     4441 acatgacaac agtaacttgt ttattagaca gaaacttgtc ggtccttcca gaatcaactt
     4501 tgttcagatt agaccaagaa gaaaaaaatt gtgatataga taccccatta ggaaatcttt
     4561 ccaaattgat ggaaaacacg tttgtcagca aaaaacttcg agatttaatt attgactttc
     4621 gtgaacctca gtttatatcc taccttagtt ctgttcttcc acatgatgca aaggatacag
     4681 ttgcctgcat tctaaagggt ttgaataagc ctcagaggca agcgatgaaa aaggtacttc
     4741 tttcaaaaga ctacacactc atcgtgggta tgcctgggac aggaaaaaca actacgatat
     4801 gtactctcgt aagaattctc tacgcctgtg gttttagcgt tttgttgacc agctatacac
     4861 actctgctgt tgacaatatt cttttgaagt tagccaagtt taaaatagga tttttgcgtt
     4921 tgggtcagat tcagaaggtt catccagcta tccagcaatt tacagagcaa gaaatttgca
     4981 gatcaaagtc cattaaatcc ttagctcttc tagaagaact ctacaatagt caacttatag
     5041 ttgcaacaac atgtatggga ataaaccatc caatattttc ccgtaaaatt tttgattttt
     5101 gtattgtgga tgaagcctct caaattagcc aaccaatttg tctgggcccc cttttttttt
     5161 cacggagatt tgtgttagtg ggggaccatc agcagcttcc tcccctggtg ctaaaccgtg
     5221 aagcaagagc tcttggcatg agtgaaagct tattcaagag gctggagcag aataagagtg
     5281 ctgttgtaca gttaaccgtg cagtacagaa tgaacagtaa aattatgtcc ttaagtaata
     5341 agctgaccta tgagggcaag ctggagtgtg gatcagacaa agtggccaat gcagtgataa
     5401 acctacgtca ctttaaagat gtgaagctgg aactggaatt ttatgctgac tattctgata
     5461 atccttggtt gatgggagta tttgaaccca acaatcctgt ttgtttcctt aatacagaca
     5521 aggttccagc gccagaacaa gttgaaaaag gtggtgtgag caatgtaaca gaagccaaac
     5581 tcatagtttt cctaacctcc atttttgtta aggctggatg cagtccctct gatattggta
     5641 ttattgcacc gtacaggcag caattaaaga tcatcaatga tttattggca cgttctattg
     5701 ggatggtcga agttaataca gtagacaaat accaaggaag ggacaaaagt attgtcctag
     5761 tatcttttgt tagaagtaat aaggatggaa ctgttggtga actcttgaaa gattggcgac
     5821 gtcttaatgt tgctataacc agagccaaac ataaactgat tcttctgggg tgtgtgccct
     5881 cactaaattg ctatcctcct ttggagaagc tgcttaatca tttaaactca gaaaaattaa
     5941 tcattgatct tccatcaaga gaacatgaaa gtctttgcca catattgggt gactttcaaa
     6001 gagaaggatc ctacccatac gacgtaccag attacgctta gtctagataa ttctaccggg
     6061 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
     6121 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
     6181 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
     6241 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
     6301 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
     6361 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
     6421 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
     6481 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
     6541 catctccggg cctttcgacc tgcagcccaa gcttaccatg accgagtaca agcccacggt
     6601 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     6661 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     6721 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     6781 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     6841 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     6901 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     6961 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     7021 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     7081 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     7141 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaccg cgtctggaac aatcaacctc
     7201 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     7261 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     7321 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     7381 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     7441 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     7501 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     7561 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
     7621 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     7681 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     7741 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattaat tctgcagtcg
     7801 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg
     7861 tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc
     7921 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaagag
     7981 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta
     8041 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
     8101 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
     8161 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
     8221 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
     8281 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
     8341 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
     8401 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
     8461 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     8521 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     8581 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     8641 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt
     8701 gctagcgttt taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     8761 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     8821 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     8881 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     8941 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     9001 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     9061 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     9121 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     9181 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     9241 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     9301 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     9361 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     9421 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     9481 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     9541 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     9601 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     9661 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     9721 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     9781 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     9841 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     9901 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     9961 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
    10021 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
    10081 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
    10141 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    10201 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    10261 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    10321 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    10381 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    10441 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    10501 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    10561 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    10621 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    10681 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    10741 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    10801 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    10861 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
    10921 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
    10981 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    11041 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
    11101 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc
    11161 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt
    11221 agtttttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt
//
