LOCUS       V030178                10705 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030178
VERSION     V030178
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2843..5401
                     /label="DNMT3B(NM_006892)"
                     /note="DNMT3B(NM_006892)"
                     /gene="DNMT3B"
     CDS             5408..5473
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5493..5992
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             6013..6612
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6626..7214
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7421..8054
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8183..8199)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8207..8223
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8231..8261)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8276..8297
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8585..9173)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9344..10204)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10205..10309)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    10357..10491
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattcgcca ccatgaaggg agacaccagg catctcaatg gagaggagga
     2881 cgccggcggg agggaagact cgatcctcgt caacggggcc tgcagcgacc agtcctccga
     2941 ctcgccccca atcctggagg ctatccgcac cccggagatc agaggccgaa gatcaagctc
     3001 gcgactctcc aagagggagg tgtccagtct gctaagctac acacaggact tgacaggcga
     3061 tggcgacggg gaagatgggg atggctctga caccccagtc atgccaaagc tcttccggga
     3121 aaccaggact cgttcagaaa gcccagctgt ccgaactcga aataacaaca gtgtctccag
     3181 ccgggagagg cacaggcctt ccccacgttc cacccgaggc cggcagggcc gcaaccatgt
     3241 ggacgagtcc cccgtggagt tcccggctac caggtccctg agacggcggg caacagcatc
     3301 ggcaggaacg ccatggccgt cccctcccag ctcttacctt accatcgacc tcacagacga
     3361 cacagaggac acacatggga cgccccagag cagcagtacc ccctacgccc gcctagccca
     3421 ggacagccag caggggggca tggagtcccc gcaggtggag gcagacagtg gagatggaga
     3481 cagttcagag tatcaggatg ggaaggagtt tggaataggg gacctcgtgt ggggaaagat
     3541 caagggcttc tcctggtggc ccgccatggt ggtgtcttgg aaggccacct ccaagcgaca
     3601 ggctatgtct ggcatgcggt gggtccagtg gtttggcgat ggcaagttct ccgaggtctc
     3661 tgcagacaaa ctggtggcac tggggctgtt cagccagcac tttaatttgg ccaccttcaa
     3721 taagctcgtc tcctatcgaa aagccatgta ccatgctctg gagaaagcta gggtgcgagc
     3781 tggcaagacc ttccccagca gccctggaga ctcattggag gaccagctga agcccatgtt
     3841 ggagtgggcc cacgggggct tcaagcccac tgggatcgag ggcctcaaac ccaacaacac
     3901 gcaaccagtg gttaataagt cgaaggtgcg tcgtgcaggc agtaggaaat tagaatcaag
     3961 gaaatacgag aacaagactc gaagacgcac agctgacgac tcagccacct ctgactactg
     4021 ccccgcaccc aagcgcctca agacaaattg ctataacaac ggcaaagacc gaggggatga
     4081 agatcagagc cgagaacaaa tggcttcaga tgttgccaac aacaagagca gcctggaaga
     4141 tggctgtttg tcttgtggca ggaaaaaccc cgtgtccttc caccctctct ttgagggggg
     4201 gctctgtcag acatgccggg atcgcttcct tgagctgttt tacatgtatg atgacgatgg
     4261 ctatcagtct tactgcactg tgtgctgcga gggccgagag ctgctgcttt gcagcaacac
     4321 gagctgctgc cggtgtttct gtgtggagtg cctggaggtg ctggtgggca caggcacagc
     4381 ggccgaggcc aagcttcagg agccctggag ctgttacatg tgtctcccgc agcgctgtca
     4441 tggcgtcctg cggcgccgga aggactggaa cgtgcgcctg caggccttct tcaccagtga
     4501 cacggggctt gaatatgaag cccccaagct gtaccctgcc attcccgcag cccgaaggcg
     4561 gcccattcga gtcctgtcat tgtttgatgg catcgcgaca ggctacctag tcctcaaaga
     4621 gttgggcata aaggtaggaa agtacgtcgc ttctgaagtg tgtgaggagt ccattgctgt
     4681 tggaaccgtg aagcacgagg ggaatatcaa atacgtgaac gacgtgagga acatcacaaa
     4741 gaaaaatatt gaagaatggg gcccatttga cttggtgatt ggcggaagcc catgcaacga
     4801 tctctcaaat gtgaatccag ccaggaaagg cctgtatgag ggtacaggcc ggctcttctt
     4861 cgaattttac cacctgctga attactcacg ccccaaggag ggtgatgacc ggccgttctt
     4921 ctggatgttt gagaatgttg tagccatgaa ggttggcgac aagagggaca tctcacggtt
     4981 cctggagtgt aatccagtga tgattgatgc catcaaagtt tctgctgctc acagggcccg
     5041 atacttctgg ggcaacctac ccgggatgaa caggcccgtg atagcatcaa agaatgataa
     5101 actcgagctg caggactgct tggaatacaa taggatagcc aagttaaaga aagtacagac
     5161 aataaccacc aagtcgaact cgatcaaaca ggggaaaaac caacttttcc ctgttgtcat
     5221 gaatggcaaa gaagatgttt tgtggtgcac tgagctcgaa aggatctttg gctttcctgt
     5281 gcactacaca gacgtgtcca acatgggccg tggtgcccgc cagaagctgc tgggaaggtc
     5341 ctggagcgtg cctgtcatcc gacacctctt cgcccctctg aaggactact ttgcatgtga
     5401 aggatccgac tacaaagacc atgacggtga ttataaagat catgacatcg actacaagga
     5461 tgacgatgac aagtagtcta gataattcta ccgggtaggg gaggcgcttt tcccaaggca
     5521 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg
     5581 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc
     5641 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc
     5701 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag
     5761 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
     5821 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg
     5881 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
     5941 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag
     6001 cccaagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     6061 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     6121 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     6181 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     6241 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     6301 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     6361 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     6421 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     6481 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     6541 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     6601 cccggtgcct gaccgcgtct ggaacaatca acctctggat tacaaaattt gtgaaagatt
     6661 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     6721 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     6781 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     6841 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     6901 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     6961 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     7021 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     7081 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     7141 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     7201 ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca tggagcaatc
     7261 acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag
     7321 gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca
     7381 gctgtagatc ttagccactt tttaaaagaa aagaggggac tggaagggct aattcactcc
     7441 caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
     7501 tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac
     7561 aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc
     7621 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg
     7681 aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc
     7741 aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg
     7801 cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct
     7861 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     7921 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     7981 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     8041 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     8101 gaaatgaata tcagagagtg agaggccttg acattgctag cgttttaccg tcgacctcta
     8161 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     8221 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     8281 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     8341 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     8401 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     8461 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     8521 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     8581 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     8641 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     8701 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     8761 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     8821 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     8881 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     8941 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     9001 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     9061 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     9121 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     9181 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     9241 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     9301 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     9361 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     9421 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     9481 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     9541 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     9601 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     9661 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     9721 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     9781 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     9841 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     9901 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     9961 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
    10021 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
    10081 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    10141 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    10201 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    10261 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    10321 gaaaagtgcc acctgacgtc gacggatcgg gagatcaact tgtttattgc agcttataat
    10381 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
    10441 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata
    10501 actcaagcta accaaaatca tcccaaactt cccaccccat accctattac cactgccaat
    10561 tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga
    10621 aattgtattt gttaaatatg tactacaaac ttagtagttt ttaaagaaat tgtatttgtt
    10681 aaatatgtac tacaaactta gtagt
//
