LOCUS V027675 9188 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V027675 VERSION V027675 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2843..3955 /label="ELF3(NM_001114309)" /note="ELF3(NM_001114309)" /gene="ELF3" promoter 3976..4475 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 4496..5095 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5109..5697 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 5904..6537 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(6666..6682) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6690..6706 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6714..6744) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6759..6780 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7068..7656) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7827..8687) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8688..8792) /gene="bla" /label="AmpR promoter" polyA_signal 8840..8974 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcgcca ccatggctgc aacctgtgag attagcaaca tttttagcaa 2881 ctacttcagt gcgatgtaca gctcggagga ctccaccctg gcctctgttc cccctgctgc 2941 cacctttggg gccgatgact tggtactgac cctgagcaac ccccagatgt cattggaggg 3001 tacagagaag gccagctggt tgggggaaca gccccagttc tggtcgaaga cgcaggttct 3061 ggactggatc agctaccaag tggagaagaa caagtacgac gcaagcgcca ttgacttctc 3121 acgatgtgac atggatggcg ccaccctctg caattgtgcc cttgaggagc tgcgtctggt 3181 ctttgggcct ctgggggacc aactccatgc ccagctgcga gacctcactt ccagctcttc 3241 tgatgagctc agttggatca ttgagctgct ggagaaggat ggcatggcct tccaggaggc 3301 cctagaccca gggccctttg accagggcag cccctttgcc caggagctgc tggacgacgg 3361 tcagcaagcc agcccctacc accccggcag ctgtggcgca ggagccccct cccctggcag 3421 ctctgacgtc tccaccgcag ggactggtgc ttctcggagc tcccactcct cagactccgg 3481 tggaagtgac gtggacctgg atcccactga tggcaagctc ttccccagcg atggttttcg 3541 tgactgcaag aagggggatc ccaagcacgg gaagcggaaa cgaggccggc cccgaaagct 3601 gagcaaagag tactgggact gtctcgaggg caagaagagc aagcacgcgc ccagaggcac 3661 ccacctgtgg gagttcatcc gggacatcct catccacccg gagctcaacg agggcctcat 3721 gaagtgggag aatcggcatg aaggcgtctt caagttcctg cgctccgagg ctgtggccca 3781 actatggggc caaaagaaaa agaacagcaa catgacctac gagaagctga gccgggccat 3841 gaggtactac tacaaacggg agatcctgga acgggtggat ggccggcgac tcgtctacaa 3901 gtttggcaaa aactcaagcg gctggaagga ggaagaggtt ctccagagtc ggaactgact 3961 ctagataatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt 4021 tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca 4081 catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta 4141 ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac 4201 aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga 4261 agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc 4321 agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg 4381 gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg 4441 cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttaccatgac 4501 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac 4561 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca 4621 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg 4681 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt 4741 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg 4801 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc 4861 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc 4921 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac 4981 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt 5041 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaccgcg 5101 tctggaacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact 5161 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg 5221 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg 5281 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa 5341 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc 5401 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg 5461 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat 5521 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt 5581 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc 5641 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg 5701 gaattaattc tgcagtcgag acctagaaaa acatggagca atcacaagta gcaatacagc 5761 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc 5821 agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca 5881 ctttttaaaa gaaaagaggg gactggaagg gctaattcac tcccaacgaa gacaagatat 5941 ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga actacacacc 6001 agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga 6061 gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag 6121 cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct 6181 agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg 6241 agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac 6301 tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt 6361 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc 6421 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg 6481 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta 6541 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga 6601 gtgagaggcc ttgacattgc tagcgtttta ccgtcgacct ctagctagag cttggcgtaa 6661 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata 6721 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta 6781 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa 6841 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg 6901 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 6961 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 7021 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 7081 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 7141 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 7201 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 7261 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 7321 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 7381 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 7441 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 7501 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 7561 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 7621 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 7681 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 7741 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 7801 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 7861 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 7921 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 7981 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 8041 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 8101 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 8161 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 8221 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 8281 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 8341 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 8401 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 8461 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 8521 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 8581 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 8641 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 8701 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 8761 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 8821 gtcgacggat cgggagatca acttgtttat tgcagcttat aatggttaca aataaagcaa 8881 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 8941 caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa 9001 tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt 9061 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat 9121 atgtactaca aacttagtag tttttaaaga aattgtattt gttaaatatg tactacaaac 9181 ttagtagt //