LOCUS V027764 10906 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V027764 VERSION V027764 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2826..5606 /label="FBXO11(NM_001190274)" /note="FBXO11(NM_001190274)" /gene="FBXO11" CDS 5607..5633 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 5694..6193 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 6214..6813 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 6827..7415 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 7622..8255 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(8384..8400) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 8408..8424 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(8432..8462) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 8477..8498 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8786..9374) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(9545..10405) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(10406..10510) /gene="bla" /label="AmpR promoter" polyA_signal 10558..10692 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ccaccatgaa ctccgtccga gccgccaacc ggagacccag gcgagtgtcg cggccgcgcc 2881 cggtgcagca acagcagcag cagcccccgc agcagccgcc gccgcagccg ccccagcagc 2941 agccgcccca gcagcagcct ccgccgccgc cgcagcagca gcagcagcag cagcctccgc 3001 cgccgccacc gccgcctccg ccgctgcctc aggagcggaa caacgtcggc gagcgggatg 3061 atgatgtgcc tgcagatatg gttgcagaag aatcaggtcc tggtgcacaa aatagtccat 3121 accaacttcg tagaaaaact cttttgccga aaagaacagc gtgtcccaca aagaacagta 3181 tggagggcgc ctcaacttca actacagaaa actttggtca tcgtgcaaaa cgtgcaagag 3241 tgtctggaaa atcacaagat ctatcagcag cacctgctga acagtatctt caggagaaac 3301 tgccagatga agtggttcta aaaatcttct cttacttgct ggaacaggat ctttgtagag 3361 cagcttgtgt atgtaaacgc ttcagtgaac ttgctaatga tccaattttg tggaaacgat 3421 tatatatgga agtatttgaa tatactcgcc ctatgatgca tcctgaacct ggaaaattct 3481 accagattaa tccagaagag tatgaacatc caaatccctg gaaagagagt ttccagcagt 3541 tgtataaagg tgcacatgta aagccaggat ttgctgaaca tttctacagt aaccctgcaa 3601 gatataaagg aagagaaaat atgttgtatt atgatactat tgaagatgcc cttggtgggg 3661 tacaagaggc tcattttgat ggacttatct ttgttcattc tggaatatat actgatgaat 3721 ggatatatat tgaatctcca atcaccatga ttggtgcagc acctgggaaa gtggcagaca 3781 aagttataat tgaaaacact agagattcaa ccttcgtttt tatggaaggc tctgaagatg 3841 cttatgttgg atatatgaca ataaggttta accctgatga caaatctgca caacaccaca 3901 atgcacacca ctgcttagag attacagtaa attgtagccc tattattgat cactgtatca 3961 tccgaagtac atgtacagtt ggttctgcag tatgtgttag tggtcaagga gcatgtccca 4021 ccatcaagca ctgtaacatc agtgactgtg aaaatgttgg actatatata acagatcatg 4081 cacagggaat atatgaggat aatgaaattt ccaataatgc gttagctggg atttgggtta 4141 aaaatcatgg aaacccaatt attagacgga atcatattca tcatggacgt gatgttggtg 4201 tgttcacatt tgatcatggc atgggttact ttgaaagttg caatatacac agaaatagga 4261 tagcaggctt tgaagtaaaa gcctatgcta accctacagt ggttcgatgt gaaattcacc 4321 atgggcagac tggaggaata tatgtccatg aaaaaggaag aggacaattc atagagaata 4381 aaatctatgc aaacaacttt gcaggtgtat ggattacctc aaatagtgac ccaacaataa 4441 ggggaaattc tatatttaat ggaaatcaag gaggagttta catctttggt gatggacgag 4501 gccttattga aggaaatgac atttatggca atgcattagc aggaattcaa attaggacaa 4561 acagttgtcc aattgttcgg cataacaaaa ttcatgatgg ccagcatggt gggatttatg 4621 tgcatgaaaa gggacaagga gtaatagaag agaatgaagt ttatagtaac actctagctg 4681 gagtctgggt gacaactggc agcactccag tactgagaag aaaccggata cacagtggca 4741 agcaggttgg tgtttatttt tatgacaatg gacatggagt gctagaagac aatgatatct 4801 ataatcatat gtattcaggg gttcagataa ggactggaag caaccccaaa attagacgca 4861 acaaaatctg gggaggacag aatggtggaa ttctagttta taattctggt ctaggctgta 4921 tagaagacaa tgaaatattt gacaatgcaa tggctggagt ctggattaag acagatagta 4981 atcctacact aagaagaaat aaaatccatg atggaagaga tggtggcatc tgtatattta 5041 atgggggtcg aggtctcctt gaagaaaatg atattttcag gaatgctcaa gcaggtgttc 5101 tcatcagcac taatagtcat ccaatcttaa ggaaaaacag aatatttgat ggatttgccg 5161 caggtattga aattacaaat cacgcaactg caacactaga aggcaatcag atttttaaca 5221 accggtttgg aggcttattt ttagcatctg gtgttaatgt gacaatgaaa gataacaaaa 5281 taatgaacaa tcaagatgcc atagaaaagg ctgttagtag aggccaatgt ttatataaaa 5341 tatcaagtta taccagctat cccatgcatg atttctacag atgtcatact tgtaacacca 5401 cagatcgaaa tgccatatgt gtgaactgca ttaagaagtg ccatcaggga catgatgtag 5461 agtttattag acatgatagg tttttctgtg actgtggtgc tggaacactg tctaatcctt 5521 gtacattagc tggtgagcct acacatgata cagatacact atatgactct gctccaccta 5581 tagaatctaa tacattgcag cacaactacc catacgatgt tccagattac gcttgaattc 5641 tgcagtcgac ggtaccgcgg gcccgggatc ccgcgactct agataattct accgggtagg 5701 ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg 5761 gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc 5821 ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt 5881 cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac 5941 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag 6001 cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc 6061 cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg 6121 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc 6181 tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc cacggtgcgc 6241 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac 6301 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg 6361 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac 6421 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc 6481 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg 6541 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc 6601 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag 6661 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc 6721 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc 6781 acctggtgca tgacccgcaa gcccggtgcc tgaccgcgtc tggaacaatc aacctctgga 6841 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg 6901 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt 6961 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag 7021 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc 7081 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga 7141 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa 7201 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac 7261 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct 7321 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca 7381 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac 7441 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc 7501 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta 7561 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga 7621 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac 7681 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca 7741 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc 7801 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg 7861 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga 7921 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc 7981 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga 8041 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg 8101 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 8161 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 8221 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat 8281 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattgcta 8341 gcgttttacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg 8401 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 8461 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 8521 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 8581 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 8641 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 8701 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 8761 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 8821 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 8881 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 8941 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 9001 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 9061 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 9121 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 9181 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta 9241 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 9301 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 9361 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 9421 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 9481 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 9541 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 9601 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 9661 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 9721 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 9781 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 9841 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 9901 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 9961 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 10021 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 10081 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 10141 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 10201 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 10261 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 10321 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 10381 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 10441 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 10501 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac 10561 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 10621 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 10681 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca 10741 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct 10801 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt 10861 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagt //