LOCUS       V027764                10906 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027764
VERSION     V027764
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2826..5606
                     /label="FBXO11(NM_001190274)"
                     /note="FBXO11(NM_001190274)"
                     /gene="FBXO11"
     CDS             5607..5633
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        5694..6193
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             6214..6813
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6827..7415
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7622..8255
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8384..8400)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8408..8424
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8432..8462)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8477..8498
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8786..9374)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9545..10405)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10406..10510)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    10558..10692
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ccaccatgaa ctccgtccga gccgccaacc ggagacccag gcgagtgtcg cggccgcgcc
     2881 cggtgcagca acagcagcag cagcccccgc agcagccgcc gccgcagccg ccccagcagc
     2941 agccgcccca gcagcagcct ccgccgccgc cgcagcagca gcagcagcag cagcctccgc
     3001 cgccgccacc gccgcctccg ccgctgcctc aggagcggaa caacgtcggc gagcgggatg
     3061 atgatgtgcc tgcagatatg gttgcagaag aatcaggtcc tggtgcacaa aatagtccat
     3121 accaacttcg tagaaaaact cttttgccga aaagaacagc gtgtcccaca aagaacagta
     3181 tggagggcgc ctcaacttca actacagaaa actttggtca tcgtgcaaaa cgtgcaagag
     3241 tgtctggaaa atcacaagat ctatcagcag cacctgctga acagtatctt caggagaaac
     3301 tgccagatga agtggttcta aaaatcttct cttacttgct ggaacaggat ctttgtagag
     3361 cagcttgtgt atgtaaacgc ttcagtgaac ttgctaatga tccaattttg tggaaacgat
     3421 tatatatgga agtatttgaa tatactcgcc ctatgatgca tcctgaacct ggaaaattct
     3481 accagattaa tccagaagag tatgaacatc caaatccctg gaaagagagt ttccagcagt
     3541 tgtataaagg tgcacatgta aagccaggat ttgctgaaca tttctacagt aaccctgcaa
     3601 gatataaagg aagagaaaat atgttgtatt atgatactat tgaagatgcc cttggtgggg
     3661 tacaagaggc tcattttgat ggacttatct ttgttcattc tggaatatat actgatgaat
     3721 ggatatatat tgaatctcca atcaccatga ttggtgcagc acctgggaaa gtggcagaca
     3781 aagttataat tgaaaacact agagattcaa ccttcgtttt tatggaaggc tctgaagatg
     3841 cttatgttgg atatatgaca ataaggttta accctgatga caaatctgca caacaccaca
     3901 atgcacacca ctgcttagag attacagtaa attgtagccc tattattgat cactgtatca
     3961 tccgaagtac atgtacagtt ggttctgcag tatgtgttag tggtcaagga gcatgtccca
     4021 ccatcaagca ctgtaacatc agtgactgtg aaaatgttgg actatatata acagatcatg
     4081 cacagggaat atatgaggat aatgaaattt ccaataatgc gttagctggg atttgggtta
     4141 aaaatcatgg aaacccaatt attagacgga atcatattca tcatggacgt gatgttggtg
     4201 tgttcacatt tgatcatggc atgggttact ttgaaagttg caatatacac agaaatagga
     4261 tagcaggctt tgaagtaaaa gcctatgcta accctacagt ggttcgatgt gaaattcacc
     4321 atgggcagac tggaggaata tatgtccatg aaaaaggaag aggacaattc atagagaata
     4381 aaatctatgc aaacaacttt gcaggtgtat ggattacctc aaatagtgac ccaacaataa
     4441 ggggaaattc tatatttaat ggaaatcaag gaggagttta catctttggt gatggacgag
     4501 gccttattga aggaaatgac atttatggca atgcattagc aggaattcaa attaggacaa
     4561 acagttgtcc aattgttcgg cataacaaaa ttcatgatgg ccagcatggt gggatttatg
     4621 tgcatgaaaa gggacaagga gtaatagaag agaatgaagt ttatagtaac actctagctg
     4681 gagtctgggt gacaactggc agcactccag tactgagaag aaaccggata cacagtggca
     4741 agcaggttgg tgtttatttt tatgacaatg gacatggagt gctagaagac aatgatatct
     4801 ataatcatat gtattcaggg gttcagataa ggactggaag caaccccaaa attagacgca
     4861 acaaaatctg gggaggacag aatggtggaa ttctagttta taattctggt ctaggctgta
     4921 tagaagacaa tgaaatattt gacaatgcaa tggctggagt ctggattaag acagatagta
     4981 atcctacact aagaagaaat aaaatccatg atggaagaga tggtggcatc tgtatattta
     5041 atgggggtcg aggtctcctt gaagaaaatg atattttcag gaatgctcaa gcaggtgttc
     5101 tcatcagcac taatagtcat ccaatcttaa ggaaaaacag aatatttgat ggatttgccg
     5161 caggtattga aattacaaat cacgcaactg caacactaga aggcaatcag atttttaaca
     5221 accggtttgg aggcttattt ttagcatctg gtgttaatgt gacaatgaaa gataacaaaa
     5281 taatgaacaa tcaagatgcc atagaaaagg ctgttagtag aggccaatgt ttatataaaa
     5341 tatcaagtta taccagctat cccatgcatg atttctacag atgtcatact tgtaacacca
     5401 cagatcgaaa tgccatatgt gtgaactgca ttaagaagtg ccatcaggga catgatgtag
     5461 agtttattag acatgatagg tttttctgtg actgtggtgc tggaacactg tctaatcctt
     5521 gtacattagc tggtgagcct acacatgata cagatacact atatgactct gctccaccta
     5581 tagaatctaa tacattgcag cacaactacc catacgatgt tccagattac gcttgaattc
     5641 tgcagtcgac ggtaccgcgg gcccgggatc ccgcgactct agataattct accgggtagg
     5701 ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg
     5761 gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc
     5821 ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt
     5881 cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac
     5941 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag
     6001 cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc
     6061 cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg
     6121 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc
     6181 tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc cacggtgcgc
     6241 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     6301 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     6361 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     6421 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     6481 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     6541 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     6601 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     6661 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     6721 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     6781 acctggtgca tgacccgcaa gcccggtgcc tgaccgcgtc tggaacaatc aacctctgga
     6841 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     6901 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     6961 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     7021 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     7081 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     7141 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     7201 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     7261 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     7321 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     7381 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac
     7441 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc
     7501 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta
     7561 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga
     7621 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac
     7681 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
     7741 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
     7801 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
     7861 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
     7921 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
     7981 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
     8041 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
     8101 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     8161 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     8221 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     8281 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattgcta
     8341 gcgttttacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     8401 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     8461 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     8521 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     8581 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     8641 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     8701 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     8761 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     8821 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     8881 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     8941 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     9001 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     9061 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     9121 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     9181 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     9241 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     9301 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     9361 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     9421 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     9481 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     9541 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     9601 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     9661 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     9721 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     9781 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     9841 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     9901 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     9961 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    10021 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    10081 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    10141 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    10201 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    10261 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    10321 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    10381 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    10441 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    10501 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac
    10561 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
    10621 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
    10681 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
    10741 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
    10801 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt
    10861 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagt
//
