LOCUS       V030634                 9731 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030634
VERSION     V030634
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2829..2855
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2859..2885
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2889..2915
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2922..4487
                     /label="FBXO7(NM_012179)"
                     /note="FBXO7(NM_012179)"
                     /gene="FBXO7"
     promoter        4519..5018
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             5039..5638
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5652..6240
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6447..7080
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(7209..7225)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7233..7249
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7257..7287)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7302..7323
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7611..8199)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8370..9230)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9231..9335)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    9383..9517
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ccaccatgta cccatacgac gtacctgatt atgcaactta tccttatgat gtacctgatt
     2881 atgctagcta tccttatgat gtcccagact acgcagaatt catgaggctg cgggtgcggc
     2941 ttctgaagcg gacctggccg ctggaggtgc ccgagacgga gccgacgctg gggcatttgc
     3001 gctcgcacct gaggcagtcc ctgctgtgca cctgggggta cagttctaat acccgattta
     3061 caattacatt gaactacaag gatcccctca ctggagatga agagaccttg gcttcatatg
     3121 ggattgtttc tggggacttg atatgtttga ttcttcaaga tgacattcca gcgcctaata
     3181 taccttcatc cacagattca gagcattctt cactccagaa taatgagcaa ccctctttgg
     3241 ccaccagctc caatcagact agcatgcagg atgaacaacc aagtgattca ttccaaggac
     3301 aggcagccca gtctggtgtt tggaatgacg acagtatgtt agggcctagt caaaattttg
     3361 aagctgagtc aattcaagat aatgcgcata tggcagaggg cacaggtttc tatccctcag
     3421 aacccatgct ctgtagtgaa tcggtggaag ggcaagtgcc acattcatta gagaccttgt
     3481 atcaatcagc tgactgttct gatgccaatg atgccttgat agtgttgata catcttctca
     3541 tgttggagtc aggttacata cctcagggca ccgaagccaa agcactgtcc atgccggaga
     3601 agtggaagtt gagcggggtg tataagctgc agtacatgca tcctctctgc gagggcagct
     3661 ccgctactct cacctgtgtg cctttgggaa acctgattgt tgtaaatgct acactaaaaa
     3721 tcaacaatga gattagaagt gtgaaaagat tgcagctgct accagaatct tttatttgca
     3781 aagagaaact aggggaaaat gtagccaaca tatacaaaga tcttcagaaa ctctctcgcc
     3841 tctttaaaga ccagctggtg tatcctcttc tggcttttac ccgacaagca ctgaacctac
     3901 cagatgtatt tgggttggtc gtcctcccat tggaactgaa actacggatc ttccgacttc
     3961 tggatgttcg ttccgtcttg tctttgtctg cggtttgtcg tgacctcttt actgcttcaa
     4021 atgacccact cctgtggagg tttttatatc tgcgtgattt tcgagacaat actgtcagag
     4081 ttcaagacac agattggaaa gaactgtaca ggaagaggca catacaaaga aaagaatccc
     4141 cgaaagggcg gtttgtgatg ctcctgccat cgtcaactca caccattcca ttctatccca
     4201 accccttgca ccctaggcca tttcctagct cccgccttcc tccaggaatt atcgggggtg
     4261 aatatgacca aagaccaaca cttccctatg ttggagaccc aatcagttca ctcattcctg
     4321 gtcctgggga gacgcccagc cagtttcctc cactgagacc acgctttgat ccagttggcc
     4381 cacttccagg acctaacccc atcttgccag ggcgaggcgg ccccaatgac agatttccct
     4441 ttagacccag caggggtcgg ccaactgatg gccggctgtc attcatgtga ggatcccgcg
     4501 actctagata attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg
     4561 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
     4621 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
     4681 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
     4741 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
     4801 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg
     4861 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg
     4921 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
     4981 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat
     5041 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     5101 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     5161 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     5221 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     5281 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     5341 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     5401 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     5461 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     5521 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     5581 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacc
     5641 gcgtctggaa caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     5701 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     5761 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     5821 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     5881 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     5941 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     6001 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     6061 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     6121 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     6181 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     6241 ctggaattaa ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
     6301 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
     6361 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     6421 ccacttttta aaagaaaaga ggggactgga agggctaatt cactcccaac gaagacaaga
     6481 tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc agaactacac
     6541 accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     6601 tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt
     6661 gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg
     6721 cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata
     6781 tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg
     6841 gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg
     6901 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     6961 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     7021 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     7081 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     7141 agagtgagag gccttgacat tgctagcgtt ttaccgtcga cctctagcta gagcttggcg
     7201 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     7261 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     7321 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     7381 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     7441 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     7501 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     7561 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7621 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7681 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7741 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7801 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7861 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7921 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7981 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     8041 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     8101 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     8161 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     8221 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     8281 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     8341 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     8401 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     8461 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     8521 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     8581 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     8641 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     8701 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8761 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8821 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     8881 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     8941 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     9001 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     9061 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     9121 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     9181 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     9241 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     9301 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     9361 gacgtcgacg gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag
     9421 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     9481 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
     9541 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
     9601 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
     9661 aatatgtact acaaacttag tagtttttaa agaaattgta tttgttaaat atgtactaca
     9721 aacttagtag t
//
