LOCUS       V020057                10505 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020057
VERSION     V020057
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2829..2855
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2856..5261
                     /label="FGFR4(NM_213647)"
                     /note="FGFR4(NM_213647)"
                     /gene="FGFR4"
     promoter        5293..5792
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             5813..6412
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6426..7014
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7221..7854
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(7983..7999)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8007..8023
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8031..8061)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8076..8097
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8385..8973)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9144..10004)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10005..10109)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    10157..10291
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ccaccatgta cccatacgac gtaccagatt acgctatgcg gctgctgctg gccctgttgg
     2881 gggtcctgct gagtgtgcct gggcctccag tcttgtccct ggaggcctct gaggaagtgg
     2941 agcttgagcc ctgcctggct cccagcctgg agcagcaaga gcaggagctg acagtagccc
     3001 ttgggcagcc tgtgcgtctg tgctgtgggc gggctgagcg tggtggccac tggtacaagg
     3061 agggcagtcg cctggcacct gctggccgtg tacggggctg gaggggccgc ctagagattg
     3121 ccagcttcct acctgaggat gctggccgct acctctgcct ggcacgaggc tccatgatcg
     3181 tcctgcagaa tctcaccttg attacaggtg actccttgac ctccagcaac gatgatgagg
     3241 accccaagtc ccatagggac ccctcgaata ggcacagtta cccccagcaa gcaccctact
     3301 ggacacaccc ccagcgcatg gagaagaaac tgcatgcagt acctgcgggg aacaccgtca
     3361 agttccgctg tccagctgca ggcaacccca cgcccaccat ccgctggctt aaggatggac
     3421 aggcctttca tggggagaac cgcattggag gcattcggct gcgccatcag cactggagtc
     3481 tcgtgatgga gagcgtggtg ccctcggacc gcggcacata cacctgcctg gtagagaacg
     3541 ctgtgggcag catccgctat aactacctgc tagatgtgct ggagcggtcc ccgcaccggc
     3601 ccatcctgca ggccgggctc ccggccaaca ccacagccgt ggtgggcagc gacgtggagc
     3661 tgctgtgcaa ggtgtacagc gatgcccagc cccacatcca gtggctgaag cacatcgtca
     3721 tcaacggcag cagcttcgga gccgacggtt tcccctatgt gcaagtccta aagactgcag
     3781 acatcaatag ctcagaggtg gaggtcctgt acctgcggaa cgtgtcagcc gaggacgcag
     3841 gcgagtacac ctgcctcgca ggcaattcca tcggcctctc ctaccagtct gcctggctca
     3901 cggtgctgcc agaggaggac cccacatgga ccgcagcagc gcccgaggcc aggtatacgg
     3961 acatcatcct gtacgcgtcg ggctccctgg ccttggctgt gctcctgctg ctggccgggc
     4021 tgtatcgagg gcaggcgctc cacggccggc acccccgccc gcccgccact gtgcagaagc
     4081 tctcccgctt ccctctggcc cgacagttct ccctggagtc aggctcttcc ggcaagtcaa
     4141 gctcatccct ggtacgaggc gtgcgtctct cctccagcgg ccccgccttg ctcgccggcc
     4201 tcgtgagtct agatctacct ctcgacccac tatgggagtt cccccgggac aggctggtgc
     4261 ttgggaagcc cctaggcgag ggctgctttg gccaggtagt acgtgcagag gcctttggca
     4321 tggaccctgc ccggcctgac caagccagca ctgtggccgt caagatgctc aaagacaacg
     4381 cctctgacaa ggacctggcc gacctggtct cggagatgga ggtgatgaag ctgatcggcc
     4441 gacacaagaa catcatcaac ctgcttggtg tctgcaccca ggaagggccc ctgtacgtga
     4501 tcgtggagtg cgccgccaag ggaaacctgc gggagttcct gcgggcccgg cgccccccag
     4561 gccccgacct cagccccgac ggtcctcgga gcagtgaggg gccgctctcc ttcccagtcc
     4621 tggtctcctg cgcctaccag gtggcccgag gcatgcagta tctggagtcc cggaagtgta
     4681 tccaccggga cctggctgcc cgcaatgtgc tggtgactga ggacaatgtg atgaagattg
     4741 ctgactttgg gctggcccgc ggcgtccacc acattgacta ctataagaaa accagcaacg
     4801 gccgcctgcc tgtgaagtgg atggcgcccg aggccttgtt tgaccgggtg tacacacacc
     4861 agagtgacgt gtggtctttt gggatcctgc tatgggagat cttcaccctc gggggctccc
     4921 cgtatcctgg catcccggtg gaggagctgt tctcgctgct gcgggaggga catcggatgg
     4981 accgaccccc acactgcccc ccagagctgt acgggctgat gcgtgagtgc tggcacgcag
     5041 cgccctccca gaggcctacc ttcaagcagc tggtggaggc gctggacaag gtcctgctgg
     5101 ccgtctctga ggagtacctc gacctccgcc tgaccttcgg accctattcc ccctctggtg
     5161 gggacgccag cagcacctgc tcctccagcg attctgtctt cagccacgac cccctgccat
     5221 tgggatccag ctccttcccc ttcgggtctg gggtgcagac atgaggatcc cgcgactcta
     5281 gataattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
     5341 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
     5401 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
     5461 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
     5521 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
     5581 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga
     5641 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg
     5701 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc
     5761 tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgaccga
     5821 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     5881 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     5941 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     6001 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     6061 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     6121 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     6181 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     6241 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     6301 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     6361 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaccgcgtct
     6421 ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     6481 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     6541 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     6601 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     6661 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     6721 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     6781 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     6841 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     6901 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     6961 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa
     7021 ttaattctgc agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
     7081 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
     7141 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     7201 tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac aagatatcct
     7261 tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg
     7321 gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc
     7381 agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct
     7441 gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc
     7501 atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc
     7561 ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg
     7621 ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc
     7681 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     7741 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     7801 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     7861 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     7921 agaggccttg acattgctag cgttttaccg tcgacctcta gctagagctt ggcgtaatca
     7981 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     8041 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     8101 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     8161 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     8221 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     8281 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     8341 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     8401 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     8461 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     8521 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     8581 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     8641 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     8701 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     8761 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     8821 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     8881 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     8941 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     9001 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     9061 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     9121 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     9181 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     9241 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     9301 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     9361 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     9421 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     9481 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     9541 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     9601 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     9661 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     9721 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     9781 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     9841 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     9901 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     9961 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    10021 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    10081 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    10141 gacggatcgg gagatcaact tgtttattgc agcttataat ggttacaaat aaagcaatag
    10201 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
    10261 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca
    10321 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact
    10381 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg
    10441 tactacaaac ttagtagttt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
    10501 gtagt
//
