LOCUS       V047391                10951 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V047391
VERSION     V047391
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2846..2863
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHH"
                     /label="6xHis"
     CDS             2864..4924
                     /label="FLT1(NM_001159920)"
                     /note="FLT1(NM_001159920)"
                     /gene="FLT1"
     CDS             4934..4990
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             5003..5722
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     promoter        5739..6238
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             6259..6858
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6872..7460
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7667..8300
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8429..8445)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8453..8469
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8477..8507)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8522..8543
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8831..9419)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9590..10450)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10451..10555)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    10603..10737
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattcgcca ccatgcatca ccatcaccat cacatggtca gctactggga
     2881 caccggggtc ctgctgtgcg cgctgctcag ctgtctgctt ctcacaggat ctagttcagg
     2941 ttcaaaatta aaagatcctg aactgagttt aaaaggcacc cagcacatca tgcaagcagg
     3001 ccagacactg catctccaat gcagggggga agcagcccat aaatggtctt tgcctgaaat
     3061 ggtgagtaag gaaagcgaaa ggctgagcat aactaaatct gcctgtggaa gaaatggcaa
     3121 acaattctgc agtactttaa ccttgaacac agctcaagca aaccacactg gcttctacag
     3181 ctgcaaatat ctagctgtac ctacttcaaa gaagaaggaa acagaatctg caatctatat
     3241 atttattagt gatacaggta gacctttcgt agagatgtac agtgaaatcc ccgaaattat
     3301 acacatgact gaaggaaggg agctcgtcat tccctgccgg gttacgtcac ctaacatcac
     3361 tgttacttta aaaaagtttc cacttgacac tttgatccct gatggaaaac gcataatctg
     3421 ggacagtaga aagggcttca tcatatcaaa tgcaacgtac aaagaaatag ggcttctgac
     3481 ctgtgaagca acagtcaatg ggcatttgta taagacaaac tatctcacac atcgacaaac
     3541 caatacaatc atagatgtcc aaataagcac accacgccca gtcaaattac ttagaggcca
     3601 tactcttgtc ctcaattgta ctgctaccac tcccttgaac acgagagttc aaatgacctg
     3661 gagttaccct gatgaaaaaa ataagagagc ttccgtaagg cgacgaattg accaaagcaa
     3721 ttcccatgcc aacatattct acagtgttct tactattgac aaaatgcaga acaaagacaa
     3781 aggactttat acttgtcgtg taaggagtgg accatcattc aaatctgtta acacctcagt
     3841 gcatatatat gataaagcat tcatcactgt gaaacatcga aaacagcagg tgcttgaaac
     3901 cgtagctggc aagcggtctt accggctctc tatgaaagtg aaggcatttc cctcgccgga
     3961 agttgtatgg ttaaaagatg ggttacctgc gactgagaaa tctgctcgct atttgactcg
     4021 tggctactcg ttaattatca aggacgtaac tgaagaggat gcagggaatt atacaatctt
     4081 gctgagcata aaacagtcaa atgtgtttaa aaacctcact gccactctaa ttgtcaatgt
     4141 gaaaccccag atttacgaaa aggccgtgtc atcgtttcca gacccggctc tctacccact
     4201 gggcagcaga caaatcctga cttgtaccgc atatggtatc cctcaaccta caatcaagtg
     4261 gttctggcac ccctgtaacc ataatcattc cgaagcaagg tgtgactttt gttccaataa
     4321 tgaagagtcc tttatcctgg atgctgacag caacatggga aacagaattg agagcatcac
     4381 tcagcgcatg gcaataatag aaggaaagaa taagatggct agcaccttgg ttgtggctga
     4441 ctctagaatt tctggaatct acatttgcat agcttccaat aaagttggga ctgtgggaag
     4501 aaacataagc ttttatatca cagatgtgcc aaatgggttt catgttaact tggaaaaaat
     4561 gccgacggaa ggagaggacc tgaaactgtc ttgcacagtt aacaagttct tatacagaga
     4621 cgttacttgg attttactgc ggacagttaa taacagaaca atgcactaca gtattagcaa
     4681 gcaaaaaatg gccatcacta aggagcactc catcactctt aatcttacca tcatgaatgt
     4741 ttccctgcaa gattcaggca cctatgcctg cagagccagg aatgtataca caggggaaga
     4801 aatcctccag aagaaagaaa ttacaatcag aggtgagcac tgcaacaaaa aggctgtttt
     4861 ctctcggatc tccaaattta aaagcacaag gaatgattgt accacacaaa gtaatgtaaa
     4921 acatggatcc ggcgcaacaa acttctctct gctgaaacaa gccggagatg tcgaagagaa
     4981 tcctggaccg ggatccgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
     5041 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     5101 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     5161 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     5221 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     5281 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     5341 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     5401 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     5461 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     5521 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     5581 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     5641 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     5701 catggacgag ctgtacaagt agtctagata attctaccgg gtaggggagg cgcttttccc
     5761 aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg
     5821 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg
     5881 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca
     5941 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat
     6001 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct
     6061 ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag
     6121 gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac
     6181 gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac
     6241 ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     6301 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     6361 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     6421 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     6481 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     6541 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     6601 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     6661 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     6721 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     6781 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     6841 cgcaagcccg gtgcctgacc gcgtctggaa caatcaacct ctggattaca aaatttgtga
     6901 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     6961 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     7021 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     7081 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     7141 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     7201 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     7261 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     7321 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     7381 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     7441 cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga
     7501 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     7561 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     7621 aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt
     7681 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     7741 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
     7801 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
     7861 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
     7921 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
     7981 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
     8041 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
     8101 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     8161 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     8221 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     8281 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     8341 tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt ttaccgtcga
     8401 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     8461 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     8521 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     8581 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     8641 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     8701 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     8761 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     8821 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     8881 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     8941 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     9001 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     9061 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     9121 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     9181 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     9241 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     9301 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     9361 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     9421 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     9481 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     9541 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     9601 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     9661 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     9721 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     9781 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     9841 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     9901 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     9961 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
    10021 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
    10081 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    10141 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    10201 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    10261 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    10321 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    10381 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    10441 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    10501 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    10561 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt tattgcagct
    10621 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
    10681 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
    10741 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
    10801 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
    10861 taaagaaatt gtatttgtta aatatgtact acaaacttag tagtttttaa agaaattgta
    10921 tttgttaaat atgtactaca aacttagtag t
//
