LOCUS V044785 10168 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V044785 VERSION V044785 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2846..2863 /codon_start=1 /product="6xHis affinity tag" /transl_table=1 /translation="HHHHHH" /label="6xHis" CDS 2864..4924 /label="FLT1(NM_001159920)" /note="FLT1(NM_001159920)" /gene="FLT1" promoter 4956..5455 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 5476..6075 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 6089..6677 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6884..7517 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(7646..7662) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7670..7686 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7694..7724) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7739..7760 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8048..8636) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8807..9667) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9668..9772) /gene="bla" /label="AmpR promoter" polyA_signal 9820..9954 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcgcca ccatgcatca ccatcaccat cacatggtca gctactggga 2881 caccggggtc ctgctgtgcg cgctgctcag ctgtctgctt ctcacaggat ctagttcagg 2941 ttcaaaatta aaagatcctg aactgagttt aaaaggcacc cagcacatca tgcaagcagg 3001 ccagacactg catctccaat gcagggggga agcagcccat aaatggtctt tgcctgaaat 3061 ggtgagtaag gaaagcgaaa ggctgagcat aactaaatct gcctgtggaa gaaatggcaa 3121 acaattctgc agtactttaa ccttgaacac agctcaagca aaccacactg gcttctacag 3181 ctgcaaatat ctagctgtac ctacttcaaa gaagaaggaa acagaatctg caatctatat 3241 atttattagt gatacaggta gacctttcgt agagatgtac agtgaaatcc ccgaaattat 3301 acacatgact gaaggaaggg agctcgtcat tccctgccgg gttacgtcac ctaacatcac 3361 tgttacttta aaaaagtttc cacttgacac tttgatccct gatggaaaac gcataatctg 3421 ggacagtaga aagggcttca tcatatcaaa tgcaacgtac aaagaaatag ggcttctgac 3481 ctgtgaagca acagtcaatg ggcatttgta taagacaaac tatctcacac atcgacaaac 3541 caatacaatc atagatgtcc aaataagcac accacgccca gtcaaattac ttagaggcca 3601 tactcttgtc ctcaattgta ctgctaccac tcccttgaac acgagagttc aaatgacctg 3661 gagttaccct gatgaaaaaa ataagagagc ttccgtaagg cgacgaattg accaaagcaa 3721 ttcccatgcc aacatattct acagtgttct tactattgac aaaatgcaga acaaagacaa 3781 aggactttat acttgtcgtg taaggagtgg accatcattc aaatctgtta acacctcagt 3841 gcatatatat gataaagcat tcatcactgt gaaacatcga aaacagcagg tgcttgaaac 3901 cgtagctggc aagcggtctt accggctctc tatgaaagtg aaggcatttc cctcgccgga 3961 agttgtatgg ttaaaagatg ggttacctgc gactgagaaa tctgctcgct atttgactcg 4021 tggctactcg ttaattatca aggacgtaac tgaagaggat gcagggaatt atacaatctt 4081 gctgagcata aaacagtcaa atgtgtttaa aaacctcact gccactctaa ttgtcaatgt 4141 gaaaccccag atttacgaaa aggccgtgtc atcgtttcca gacccggctc tctacccact 4201 gggcagcaga caaatcctga cttgtaccgc atatggtatc cctcaaccta caatcaagtg 4261 gttctggcac ccctgtaacc ataatcattc cgaagcaagg tgtgactttt gttccaataa 4321 tgaagagtcc tttatcctgg atgctgacag caacatggga aacagaattg agagcatcac 4381 tcagcgcatg gcaataatag aaggaaagaa taagatggct agcaccttgg ttgtggctga 4441 ctctagaatt tctggaatct acatttgcat agcttccaat aaagttggga ctgtgggaag 4501 aaacataagc ttttatatca cagatgtgcc aaatgggttt catgttaact tggaaaaaat 4561 gccgacggaa ggagaggacc tgaaactgtc ttgcacagtt aacaagttct tatacagaga 4621 cgttacttgg attttactgc ggacagttaa taacagaaca atgcactaca gtattagcaa 4681 gcaaaaaatg gccatcacta aggagcactc catcactctt aatcttacca tcatgaatgt 4741 ttccctgcaa gattcaggca cctatgcctg cagagccagg aatgtataca caggggaaga 4801 aatcctccag aagaaagaaa ttacaatcag aggtgagcac tgcaacaaaa aggctgtttt 4861 ctctcggatc tccaaattta aaagcacaag gaatgattgt accacacaaa gtaatgtaaa 4921 acattaagga tcccgcgact ctagataatt ctaccgggta ggggaggcgc ttttcccaag 4981 gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct 5041 ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg 5101 ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct 5161 cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga 5221 cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg 5281 ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg 5341 cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct 5401 tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg 5461 cagcccaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac 5521 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac 5581 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg 5641 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc 5701 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg 5761 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg 5821 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag 5881 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg 5941 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc 6001 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc 6061 aagcccggtg cctgaccgcg tctggaacaa tcaacctctg gattacaaaa tttgtgaaag 6121 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat 6181 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc 6241 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg 6301 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct 6361 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct 6421 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg 6481 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac 6541 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct 6601 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct 6661 ttgggccgcc tccccgcctg gaattaattc tgcagtcgag acctagaaaa acatggagca 6721 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag 6781 gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag 6841 gcagctgtag atcttagcca ctttttaaaa gaaaagaggg gactggaagg gctaattcac 6901 tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct 6961 gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc 7021 tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc 7081 agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag 7141 tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac 7201 ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg 7261 aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct 7321 gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg 7381 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt 7441 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt 7501 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc 7561 aaagaaatga atatcagaga gtgagaggcc ttgacattgc tagcgtttta ccgtcgacct 7621 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc 7681 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat 7741 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc 7801 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg 7861 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 7921 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 7981 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 8041 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 8101 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 8161 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 8221 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 8281 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 8341 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 8401 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 8461 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc 8521 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 8581 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 8641 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 8701 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 8761 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 8821 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 8881 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 8941 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 9001 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 9061 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 9121 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 9181 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 9241 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 9301 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 9361 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 9421 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 9481 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 9541 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 9601 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 9661 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 9721 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 9781 cccgaaaagt gccacctgac gtcgacggat cgggagatca acttgtttat tgcagcttat 9841 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 9901 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg 9961 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc 10021 aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa 10081 agaaattgta tttgttaaat atgtactaca aacttagtag tttttaaaga aattgtattt 10141 gttaaatatg tactacaaac ttagtagt //