LOCUS V022884 10064 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V022884 VERSION V022884 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2827..4791 /label="FOXO1(NM_002015)" /note="FOXO1(NM_002015)" /gene="FOXO1" promoter 4852..5351 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 5372..5971 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5985..6573 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6780..7413 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(7542..7558) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7566..7582 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7590..7620) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7635..7656 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7944..8532) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8703..9563) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9564..9668) /gene="bla" /label="AmpR promoter" polyA_signal 9716..9850 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 gccaccatgg ccgaggcgcc tcaggtggtg gagatcgacc cggacttcga gccgctgccc 2881 cggccgcgct cgtgcacctg gccgctgccc aggccggagt ttagccagtc caactcggcc 2941 acctccagcc cggcgccgtc gggcagcgcg gctgccaacc ccgacgccgc ggcgggcctg 3001 ccctcggcct cggctgccgc tgtcagcgcc gacttcatga gcaacctgag cttgctggag 3061 gagagcgagg acttcccgca ggcgcccggc tccgtggcgg cggcggtggc ggcggcggcc 3121 gccgcggccg ccaccggggg gctgtgcggg gacttccagg gcccggaggc gggctgcctg 3181 cacccagcgc caccgcagcc cccgccgccc gggccgctgt cgcagcaccc gccggtgccc 3241 cccgccgccg ctgggccgct cgcggggcag ccgcgcaaga gcagctcgtc ccgccgcaac 3301 gcgtggggca acctgtccta cgccgacctc atcaccaagg ccatcgagag ctcggcggag 3361 aagcggctca cgctgtcgca gatctacgag tggatggtca agagcgtgcc ctacttcaag 3421 gataagggtg acagcaacag ctcggcgggc tggaagaatc gaattcgtca taatctgtcc 3481 ctacacagca agttcattcg tgtgcagaat gaaggaactg gaaaaagttc ttggtggatg 3541 ctcaatccag agggtggcaa gagcgggaaa tctcctagga gaagagctgc atccatggac 3601 aacaacagta aatttgctaa gagccgaagc cgagctgcca agaagaaagc atctctccag 3661 tctggccagg agggtgctgg ggacagccct ggatcacagt tttccaaatg gcctgcaagc 3721 cctggctctc acagcaatga tgactttgat aactggagta catttcgccc tcgaactagc 3781 tcaaatgcta gtactattag tgggagactc tcacccatta tgaccgaaca ggatgatctt 3841 ggagaagggg atgtgcattc tatggtgtac ccgccatctg ccgcaaagat ggcctctact 3901 ttacccagtc tgtctgagat aagcaatccc gaaaacatgg aaaatctttt ggataatctc 3961 aaccttctct catcaccaac atcattaact gtttcgaccc agtcctcacc tggcaccatg 4021 atgcagcaga cgccgtgcta ctcgtttgcg ccaccaaaca ccagtttgaa ttcacccagc 4081 ccaaactacc aaaaatatac atatggccaa tccagcatga gccctttgcc ccagatgcct 4141 atacaaacac ttcaggacaa taagtcgagt tatggaggta tgagtcagta taactgtgcg 4201 cctggactct tgaaggagtt gctgacttct gactctcctc cccataatga cattatgaca 4261 ccagttgatc ctggggtagc ccagcccaac agccgggttc tgggccagaa cgtcatgatg 4321 ggccctaatt cggtcatgtc aacctatggc agccaggcat ctcataacaa aatgatgaat 4381 cccagctccc atacccaccc tggacatgct cagcagacat ctgcagttaa cgggcgtccc 4441 ctgccccaca cggtaagcac catgccccac acctcgggta tgaaccgcct gacccaagtg 4501 aagacacctg tacaagtgcc tctgccccac cccatgcaga tgagtgccct ggggggctac 4561 tcctccgtga gcagctgcaa tggctatggc agaatgggcc ttctccacca ggagaagctc 4621 ccaagtgact tggatggcat gttcattgag cgcttagact gtgacatgga atccatcatt 4681 cggaatgacc tcatggatgg agatacattg gattttaact ttgacaatgt gttgcccaac 4741 caaagcttcc cacacagtgt caagacaacg acacatagct gggtgtcagg ctgaattctg 4801 cagtcgacgg taccgcgggc ccgggatccc gcgactctag ataattctac cgggtagggg 4861 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc 4921 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg 4981 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc 5041 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta 5101 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg 5161 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg 5221 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc 5281 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc 5341 cgggcctttc gacctgcagc ccaagcttac catgaccgag tacaagccca cggtgcgcct 5401 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta 5461 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca 5521 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg 5581 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga 5641 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga 5701 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt 5761 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc 5821 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt 5881 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac 5941 ctggtgcatg acccgcaagc ccggtgcctg accgcgtctg gaacaatcaa cctctggatt 6001 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg 6061 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct 6121 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc 6181 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca 6241 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac 6301 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt 6361 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct 6421 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc 6481 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga 6541 cgagtcggat ctccctttgg gccgcctccc cgcctggaat taattctgca gtcgagacct 6601 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg 6661 gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag 6721 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa agaggggact 6781 ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac 6841 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact 6901 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa 6961 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga 7021 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga 7081 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc 7141 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc 7201 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag 7261 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt 7321 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca 7381 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc 7441 agtatttata acttgcaaag aaatgaatat cagagagtga gaggccttga cattgctagc 7501 gttttaccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg 7561 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa 7621 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct 7681 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 7741 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 7801 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 7861 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 7921 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 7981 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 8041 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 8101 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 8161 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 8221 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 8281 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 8341 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc 8401 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 8461 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 8521 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 8581 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 8641 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 8701 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 8761 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 8821 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 8881 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 8941 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 9001 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 9061 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 9121 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 9181 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 9241 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 9301 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 9361 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 9421 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 9481 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 9541 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 9601 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 9661 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcaactt 9721 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 9781 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 9841 tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata 9901 ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga 9961 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagtttt 10021 taaagaaatt gtatttgtta aatatgtact acaaacttag tagt //