LOCUS       V022884                10064 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V022884
VERSION     V022884
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2827..4791
                     /label="FOXO1(NM_002015)"
                     /note="FOXO1(NM_002015)"
                     /gene="FOXO1"
     promoter        4852..5351
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             5372..5971
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5985..6573
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6780..7413
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(7542..7558)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7566..7582
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7590..7620)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7635..7656
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7944..8532)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8703..9563)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9564..9668)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    9716..9850
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 gccaccatgg ccgaggcgcc tcaggtggtg gagatcgacc cggacttcga gccgctgccc
     2881 cggccgcgct cgtgcacctg gccgctgccc aggccggagt ttagccagtc caactcggcc
     2941 acctccagcc cggcgccgtc gggcagcgcg gctgccaacc ccgacgccgc ggcgggcctg
     3001 ccctcggcct cggctgccgc tgtcagcgcc gacttcatga gcaacctgag cttgctggag
     3061 gagagcgagg acttcccgca ggcgcccggc tccgtggcgg cggcggtggc ggcggcggcc
     3121 gccgcggccg ccaccggggg gctgtgcggg gacttccagg gcccggaggc gggctgcctg
     3181 cacccagcgc caccgcagcc cccgccgccc gggccgctgt cgcagcaccc gccggtgccc
     3241 cccgccgccg ctgggccgct cgcggggcag ccgcgcaaga gcagctcgtc ccgccgcaac
     3301 gcgtggggca acctgtccta cgccgacctc atcaccaagg ccatcgagag ctcggcggag
     3361 aagcggctca cgctgtcgca gatctacgag tggatggtca agagcgtgcc ctacttcaag
     3421 gataagggtg acagcaacag ctcggcgggc tggaagaatc gaattcgtca taatctgtcc
     3481 ctacacagca agttcattcg tgtgcagaat gaaggaactg gaaaaagttc ttggtggatg
     3541 ctcaatccag agggtggcaa gagcgggaaa tctcctagga gaagagctgc atccatggac
     3601 aacaacagta aatttgctaa gagccgaagc cgagctgcca agaagaaagc atctctccag
     3661 tctggccagg agggtgctgg ggacagccct ggatcacagt tttccaaatg gcctgcaagc
     3721 cctggctctc acagcaatga tgactttgat aactggagta catttcgccc tcgaactagc
     3781 tcaaatgcta gtactattag tgggagactc tcacccatta tgaccgaaca ggatgatctt
     3841 ggagaagggg atgtgcattc tatggtgtac ccgccatctg ccgcaaagat ggcctctact
     3901 ttacccagtc tgtctgagat aagcaatccc gaaaacatgg aaaatctttt ggataatctc
     3961 aaccttctct catcaccaac atcattaact gtttcgaccc agtcctcacc tggcaccatg
     4021 atgcagcaga cgccgtgcta ctcgtttgcg ccaccaaaca ccagtttgaa ttcacccagc
     4081 ccaaactacc aaaaatatac atatggccaa tccagcatga gccctttgcc ccagatgcct
     4141 atacaaacac ttcaggacaa taagtcgagt tatggaggta tgagtcagta taactgtgcg
     4201 cctggactct tgaaggagtt gctgacttct gactctcctc cccataatga cattatgaca
     4261 ccagttgatc ctggggtagc ccagcccaac agccgggttc tgggccagaa cgtcatgatg
     4321 ggccctaatt cggtcatgtc aacctatggc agccaggcat ctcataacaa aatgatgaat
     4381 cccagctccc atacccaccc tggacatgct cagcagacat ctgcagttaa cgggcgtccc
     4441 ctgccccaca cggtaagcac catgccccac acctcgggta tgaaccgcct gacccaagtg
     4501 aagacacctg tacaagtgcc tctgccccac cccatgcaga tgagtgccct ggggggctac
     4561 tcctccgtga gcagctgcaa tggctatggc agaatgggcc ttctccacca ggagaagctc
     4621 ccaagtgact tggatggcat gttcattgag cgcttagact gtgacatgga atccatcatt
     4681 cggaatgacc tcatggatgg agatacattg gattttaact ttgacaatgt gttgcccaac
     4741 caaagcttcc cacacagtgt caagacaacg acacatagct gggtgtcagg ctgaattctg
     4801 cagtcgacgg taccgcgggc ccgggatccc gcgactctag ataattctac cgggtagggg
     4861 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc
     4921 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg
     4981 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc
     5041 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta
     5101 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg
     5161 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg
     5221 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc
     5281 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc
     5341 cgggcctttc gacctgcagc ccaagcttac catgaccgag tacaagccca cggtgcgcct
     5401 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     5461 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     5521 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     5581 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     5641 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     5701 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     5761 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     5821 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     5881 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     5941 ctggtgcatg acccgcaagc ccggtgcctg accgcgtctg gaacaatcaa cctctggatt
     6001 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     6061 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     6121 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     6181 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     6241 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     6301 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     6361 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     6421 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     6481 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     6541 cgagtcggat ctccctttgg gccgcctccc cgcctggaat taattctgca gtcgagacct
     6601 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg
     6661 gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag
     6721 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa agaggggact
     6781 ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac
     6841 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
     6901 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
     6961 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     7021 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
     7081 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
     7141 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
     7201 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
     7261 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     7321 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     7381 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     7441 agtatttata acttgcaaag aaatgaatat cagagagtga gaggccttga cattgctagc
     7501 gttttaccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     7561 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     7621 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     7681 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     7741 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     7801 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     7861 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     7921 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     7981 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     8041 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     8101 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     8161 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     8221 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     8281 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     8341 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     8401 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     8461 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     8521 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     8581 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     8641 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     8701 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     8761 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     8821 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     8881 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     8941 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     9001 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     9061 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     9121 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     9181 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     9241 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     9301 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     9361 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     9421 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     9481 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     9541 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     9601 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     9661 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcaactt
     9721 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     9781 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     9841 tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata
     9901 ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga
     9961 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagtttt
    10021 taaagaaatt gtatttgtta aatatgtact acaaacttag tagt
//
