LOCUS       V019408                 9810 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V019408
VERSION     V019408
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2827..4542
                     /label="FUBP3(NM_003934)"
                     /note="FUBP3(NM_003934)"
                     /gene="FUBP3"
     CDS             4543..4566
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     promoter        4598..5097
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             5118..5717
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5731..6319
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6526..7159
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(7288..7304)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7312..7328
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7336..7366)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7381..7402
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7690..8278)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8449..9309)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9310..9414)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    9462..9596
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 gccaccatgg cggagctggt gcaggggcag agcgctcctg tggggatgaa ggccgagggc
     2881 ttcgtggatg ccctgcaccg ggtccggcag attgctgcta aaattgattc aattcctcac
     2941 ttgaataatt ccacacctct agtggacccc tcagtatatg gatacggagt acaaaaacgg
     3001 cccttggatg atggagtagg taaccagtta ggggccttgg tacatcaaag gacggtaata
     3061 acggaagaat tcaaagtgcc tgacaaaatg gttggattta ttatcggcag gggaggtgag
     3121 cagatttcac ggattcaagc agaatctggt tgcaaaattc agattgcttc agagagttct
     3181 gggattccag agaggccctg tgtacttacc ggaaccccag aaagtattga acaagccaaa
     3241 cggctcctgg gacagattgt ggaccgctgt cgaaatggac ctggctttca taatgacata
     3301 gacagcaaca gcacaatcca ggagattctc attcccgcat ctaaagtggg tctggtcatc
     3361 ggcagaggag gggaaacaat caagcagttg caggagcgga caggggtgaa gatggtcatg
     3421 atccaggatg gcccattgcc cacgggagca gacaagcctc ttcgtatcac tggagatgca
     3481 tttaaagtac agcaagcaag agaaatggta ctagagatta tccgagaaaa agaccaagct
     3541 gactttcggg gtgtacgcgg cgatttcaac tctcgaatgg gaggaggcag tatagaggta
     3601 tctgtgccta ggtttgctgt ggggattgta ataggaagaa acggggaaat gatcaaaaag
     3661 atccagaatg atgctggtgt gaggattcag tttaaaccag atgatgggat tagtccagaa
     3721 agagctgccc aggtcatggg ccctccggat cggtgtcagc atgcagcgca tatcatcagc
     3781 gagctgattc ttacagccca ggaaagagac ggctttggag gcctggcagc agccagagga
     3841 agaggtcgtg gccgtggcga ctggagcgtg ggagcccctg gtggcgtcca ggagataaca
     3901 tacacggtgc cagccgataa gtgtggcctc gtcataggca aagggggtga gaacatcaaa
     3961 agcatcaacc agcagtcagg ggcgcacgtg gagcttcaga ggaacccccc tcccaacagc
     4021 gaccccaacc tgcggagatt caccatcagg ggggttcccc agcagatcga ggtggccagg
     4081 cagctcatag atgagaaagt tggcgggacc aatctcggag cacctggagc cttcggacag
     4141 agtccattca gccagccacc tgccccacct catcaaaata cctttcctcc aaggagctcc
     4201 gggtgcttcc caaacatggc tgccaaggtg aatgggaacc cccacagcac ccctgtgagt
     4261 ggtcctccgg cctttctgac ccagggctgg ggcagcacct accaggcgtg gcagcagccc
     4321 acacagcagg tcccaagcca gcagagccag ccgcagagca gccagcccaa ctacagcaag
     4381 gcctgggaag actattacaa aaaacagagt cacgccgcca gcgctgctcc tcaggccagc
     4441 tccccaccgg actacacaat ggcctgggca gaatattaca gacagcaggt cgctttctac
     4501 ggacagacgt tagggcaggc gcaggcccac agccaggagc aggattacaa ggatgacgac
     4561 gataagtagg gatcccgcga ctctagataa ttctaccggg taggggaggc gcttttccca
     4621 aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc
     4681 ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt
     4741 ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag
     4801 ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg
     4861 gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt
     4921 tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg
     4981 ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg
     5041 cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc
     5101 tgcagcccaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
     5161 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
     5221 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
     5281 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
     5341 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
     5401 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
     5461 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
     5521 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
     5581 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
     5641 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
     5701 gcaagcccgg tgcctgaccg cgtctggaac aatcaacctc tggattacaa aatttgtgaa
     5761 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     5821 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     5881 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     5941 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     6001 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     6061 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     6121 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     6181 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     6241 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     6301 ctttgggccg cctccccgcc tggaattaat tctgcagtcg agacctagaa aaacatggag
     6361 caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag
     6421 aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca
     6481 aggcagctgt agatcttagc cactttttaa aagaaaagag gggactggaa gggctaattc
     6541 actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
     6601 ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt
     6661 gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca
     6721 ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag
     6781 agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt
     6841 acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag
     6901 ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag
     6961 ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     7021 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     7081 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     7141 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     7201 gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gctagcgttt taccgtcgac
     7261 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     7321 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     7381 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     7441 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     7501 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     7561 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     7621 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     7681 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7741 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     7801 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     7861 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     7921 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     7981 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     8041 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     8101 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     8161 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     8221 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     8281 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     8341 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     8401 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     8461 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     8521 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     8581 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     8641 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     8701 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     8761 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     8821 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     8881 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     8941 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     9001 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     9061 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     9121 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     9181 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     9241 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     9301 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     9361 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     9421 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat caacttgttt attgcagctt
     9481 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     9541 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact
     9601 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg
     9661 ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
     9721 aaagaaattg tatttgttaa atatgtacta caaacttagt agtttttaaa gaaattgtat
     9781 ttgttaaata tgtactacaa acttagtagt
//
