LOCUS       V034807                11410 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034807
VERSION     V034807
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2843..3559
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             3629..6106
                     /label="HIF1A(NM_001530)"
                     /note="HIF1A(NM_001530)"
                     /gene="HIF1A"
     CDS             6113..6178
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6198..6697
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             6718..7317
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7331..7919
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8126..8759
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8888..8904)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8912..8928
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8936..8966)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8981..9002
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9290..9878)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10049..10909)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10910..11014)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    11062..11196
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
     2881 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     2941 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     3001 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     3061 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     3121 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     3181 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     3241 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     3301 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     3361 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     3421 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     3481 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     3541 catggacgag ctgtacaagt ccggactcag atctcgaggc ggcggaggga gtggaggagg
     3601 gggaagcgga ggcggaggga gtggatccat ggagggcgcc ggcggcgcga acgacaagaa
     3661 aaagataagt tctgaacgtc gaaaagaaaa gtctcgagat gcagccagat ctcggcgaag
     3721 taaagaatct gaagtttttt atgagcttgc tcatcagttg ccacttccac ataatgtgag
     3781 ttcgcatctt gataaggcct ctgtgatgag gcttaccatc agctatttgc gtgtgaggaa
     3841 acttctggat gctggtgatt tggatattga agatgacatg aaagcacaga tgaattgctt
     3901 ttatttgaaa gccttggatg gttttgttat ggttctcaca gatgatggtg acatgattta
     3961 catttctgat aatgtgaaca aatacatggg attaactcag tttgaactaa ctggacacag
     4021 tgtgtttgat tttactcatc catgtgacca tgaggaaatg agagaaatgc ttacacacag
     4081 aaatggcctt gtgaaaaagg gtaaagaaca aaacacacag cgaagctttt ttctcagaat
     4141 gaagtgtacc ctaactagcc gaggaagaac tatgaacata aagtctgcaa catggaaggt
     4201 attgcactgc acaggccaca ttcacgtata tgataccaac agtaaccaac ctcagtgtgg
     4261 gtataagaaa ccacctatga cctgcttggt gctgatttgt gaacccattc ctcacccatc
     4321 aaatattgaa attcctttag atagcaagac tttcctcagt cgacacagcc tggatatgaa
     4381 attttcttat tgtgatgaaa gaattaccga attgatggga tatgagccag aagaactttt
     4441 aggccgctca atttatgaat attatcatgc tttggactct gatcatctga ccaaaactca
     4501 tcatgatatg tttactaaag gacaagtcac cacaggacag tacaggatgc ttgccaaaag
     4561 aggtggatat gtctgggttg aaactcaagc aactgtcata tataacacca agaattctca
     4621 accacagtgc attgtatgtg tgaattacgt tgtgagtggt attattcagc acgacttgat
     4681 tttctccctt caacaaacag aatgtgtcct taaaccggtt gaatcttcag atatgaaaat
     4741 gactcagcta ttcaccaaag ttgaatcaga agatacaagt agcctctttg acaaacttaa
     4801 gaaggaacct gatgctttaa ctttgctggc cccagccgct ggagacacaa tcatatcttt
     4861 agattttggc agcaacgaca cagaaactga tgaccagcaa cttgaggaag taccattata
     4921 taatgatgta atgctcccct cacccaacga aaaattacag aatataaatt tggcaatgtc
     4981 tccattaccc accgctgaaa cgccaaagcc acttcgaagt agtgctgacc ctgcactcaa
     5041 tcaagaagtt gcattaaaat tagaaccaaa tccagagtca ctggaacttt cttttaccat
     5101 gccccagatt caggatcaga cacctagtcc ttccgatgga agcactagac aaagttcacc
     5161 tgagcctaat agtcccagtg aatattgttt ttatgtggat agtgatatgg tcaatgaatt
     5221 caagttggaa ttggtagaaa aactttttgc tgaagacaca gaagcaaaga acccattttc
     5281 tactcaggac acagatttag acttggagat gttagctccc tatatcccaa tggatgatga
     5341 cttccagtta cgttccttcg atcagttgtc accattagaa agcagttccg caagccctga
     5401 aagcgcaagt cctcaaagca cagttacagt attccagcag actcaaatac aagaacctac
     5461 tgctaatgcc accactacca ctgccaccac tgatgaatta aaaacagtga caaaagaccg
     5521 tatggaagac attaaaatat tgattgcatc tccatctcct acccacatac ataaagaaac
     5581 tactagtgcc acatcatcac catatagaga tactcaaagt cggacagcct caccaaacag
     5641 agcaggaaaa ggagtcatag aacagacaga aaaatctcat ccaagaagcc ctaacgtgtt
     5701 atctgtcgct ttgagtcaaa gaactacagt tcctgaggaa gaactaaatc caaagatact
     5761 agctttgcag aatgctcaga gaaagcgaaa aatggaacat gatggttcac tttttcaagc
     5821 agtaggaatt ggaacattat tacagcagcc agacgatcat gcagctacta catcactttc
     5881 ttggaaacgt gtaaaaggat gcaaatctag tgaacagaat ggaatggagc aaaagacaat
     5941 tattttaata ccctctgatt tagcatgtag actgctgggg caatcaatgg atgaaagtgg
     6001 attaccacag ctgaccagtt atgattgtga agttaatgct cctatacaag gcagcagaaa
     6061 cctactgcag ggtgaagaat tactcagagc tttggatcaa gttaacggat ccgactacaa
     6121 agaccatgac ggtgattata aagatcatga catcgactac aaggatgacg atgacaagta
     6181 gtctagataa ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc
     6241 tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc
     6301 cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc
     6361 tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg
     6421 acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg
     6481 gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc
     6541 tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc
     6601 gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc
     6661 cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagcccaa gcttaccatg
     6721 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     6781 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     6841 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     6901 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     6961 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     7021 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     7081 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     7141 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     7201 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     7261 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaccg
     7321 cgtctggaac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     7381 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     7441 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     7501 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     7561 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     7621 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     7681 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     7741 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     7801 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     7861 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     7921 tggaattaat tctgcagtcg agacctagaa aaacatggag caatcacaag tagcaataca
     7981 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
     8041 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     8101 cactttttaa aagaaaagag gggactggaa gggctaattc actcccaacg aagacaagat
     8161 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca
     8221 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     8281 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
     8341 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc
     8401 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat
     8461 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg
     8521 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg
     8581 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     8641 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     8701 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     8761 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     8821 gagtgagagg ccttgacatt gctagcgttt taccgtcgac ctctagctag agcttggcgt
     8881 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     8941 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     9001 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     9061 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     9121 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     9181 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     9241 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     9301 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     9361 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     9421 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     9481 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     9541 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     9601 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     9661 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     9721 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     9781 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     9841 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     9901 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     9961 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
    10021 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
    10081 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
    10141 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
    10201 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
    10261 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
    10321 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
    10381 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
    10441 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    10501 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    10561 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    10621 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
    10681 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    10741 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    10801 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    10861 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    10921 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    10981 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    11041 acgtcgacgg atcgggagat caacttgttt attgcagctt ataatggtta caaataaagc
    11101 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
    11161 tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa
    11221 aatcatccca aacttcccac cccataccct attaccactg ccaattacct gtggtttcat
    11281 ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa
    11341 atatgtacta caaacttagt agtttttaaa gaaattgtat ttgttaaata tgtactacaa
    11401 acttagtagt
//
