LOCUS       V027917                11769 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027917
VERSION     V027917
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2821..5184
                     /label="ITGB3(NM_000212)"
                     /note="ITGB3(NM_000212)"
                     /gene="ITGB3"
     CDS             5191..5214
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     CDS             5224..5277
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     promoter        6557..7056
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             7077..7676
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7690..8278
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8485..9118
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(9247..9263)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9271..9287
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9295..9325)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9340..9361
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9649..10237)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10408..11268)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11269..11373)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    11421..11555
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 atgcgagcgc ggccgcggcc ccggccgctc tgggcgactg tgctggcgct gggggcgctg
     2881 gcgggcgttg gcgtaggagg gcccaacatc tgtaccacgc gaggtgtgag ctcctgccag
     2941 cagtgcctgg ctgtgagccc catgtgtgcc tggtgctctg atgaggccct gcctctgggc
     3001 tcacctcgct gtgacctgaa ggagaatctg ctgaaggata actgtgcccc agaatccatc
     3061 gagttcccag tgagtgaggc ccgagtacta gaggacaggc ccctcagcga caagggctct
     3121 ggagacagct cccaggtcac tcaagtcagt ccccagagga ttgcactccg gctccggcca
     3181 gatgattcga agaatttctc catccaagtg cggcaggtgg aggattaccc tgtggacatc
     3241 tactacttga tggacctgtc ttactccatg aaggatgatc tgtggagcat ccagaacctg
     3301 ggtaccaagc tggccaccca gatgcgaaag ctcaccagta acctgcggat tggcttcggg
     3361 gcatttgtgg acaagcctgt gtcaccatac atgtatatct ccccaccaga ggccctcgaa
     3421 aacccctgct atgatatgaa gaccacctgc ttgcccatgt ttggctacaa acacgtgctg
     3481 acgctaactg accaggtgac ccgcttcaat gaggaagtga agaagcagag tgtgtcacgg
     3541 aaccgagatg ccccagaggg tggctttgat gccatcatgc aggctacagt ctgtgatgaa
     3601 aagattggct ggaggaatga tgcatcccac ttgctggtgt ttaccactga tgccaagact
     3661 catatagcat tggacggaag gctggcaggc attgtccagc ctaatgacgg gcagtgtcat
     3721 gttggtagtg acaatcatta ctctgcctcc actaccatgg attatccctc tttggggctg
     3781 atgactgaga agctatccca gaaaaacatc aatttgatct ttgcagtgac tgaaaatgta
     3841 gtcaatctct atcagaacta tagtgagctc atcccaggga ccacagttgg ggttctgtcc
     3901 atggattcca gcaatgtcct ccagctcatt gttgatgctt atgggaaaat ccgttctaaa
     3961 gtagagctgg aagtgcgtga cctccctgaa gagttgtctc tatccttcaa tgccacctgc
     4021 ctcaacaatg aggtcatccc tggcctcaag tcttgtatgg gactcaagat tggagacacg
     4081 gtgagcttca gcattgaggc caaggtgcga ggctgtcccc aggagaagga gaagtccttt
     4141 accataaagc ccgtgggctt caaggacagc ctgatcgtcc aggtcacctt tgattgtgac
     4201 tgtgcctgcc aggcccaagc tgaacctaat agccatcgct gcaacaatgg caatgggacc
     4261 tttgagtgtg gggtatgccg ttgtgggcct ggctggctgg gatcccagtg tgagtgctca
     4321 gaggaggact atcgcccttc ccagcaggac gaatgcagcc cccgggaggg tcagcccgtc
     4381 tgcagccagc ggggcgagtg cctctgtggt caatgtgtct gccacagcag tgactttggc
     4441 aagatcacgg gcaagtactg cgagtgtgac gacttctcct gtgtccgcta caagggggag
     4501 atgtgctcag gccatggcca gtgcagctgt ggggactgcc tgtgtgactc cgactggacc
     4561 ggctactact gcaactgtac cacgcgtact gacacctgca tgtccagcaa tgggctgctg
     4621 tgcagcggcc gcggcaagtg tgaatgtggc agctgtgtct gtatccagcc gggctcctat
     4681 ggggacacct gtgagaagtg ccccacctgc ccagatgcct gcacctttaa gaaagaatgt
     4741 gtggagtgta agaagtttga ccggggagcc ctacatgacg aaaatacctg caaccgttac
     4801 tgccgtgacg agattgagtc agtgaaagag cttaaggaca ctggcaagga tgcagtgaat
     4861 tgtacctata agaatgagga tgactgtgtc gtcagattcc agtactatga agattctagt
     4921 ggaaagtcca tcctgtatgt ggtagaagag ccagagtgtc ccaagggccc tgacatcctg
     4981 gtggtcctgc tctcagtgat gggggccatt ctgctcattg gccttgccgc cctgctcatc
     5041 tggaaactcc tcatcaccat ccacgaccga aaagaattcg ctaaatttga ggaagaacgc
     5101 gccagagcaa aatgggacac agccaacaac ccactgtata aagaggccac gtctaccttc
     5161 accaatatca cgtaccgggg cactgaattc gattacaagg atgacgacga taagggcggt
     5221 tcagagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctggt
     5281 ggatctatgc tgctatccgt gccgctgctg ctcggcctcc tcggcctggc cgtcgccgag
     5341 cctgccgtct acttcaagga gcagtttctg gacggagacg ggtggacttc ccgctggatc
     5401 gaatccaaac acaagtcaga ttttggcaaa ttcgttctca gttccggcaa gttctacggt
     5461 gacgaggaga aagataaagg tttgcagaca agccaggatg cacgctttta tgctctgtcg
     5521 gccagtttcg agcctttcag caacaaaggc cagacgctgg tggtgcagtt cacggtgaaa
     5581 catgagcaga acatcgactg tgggggcggc tatgtgaagc tgtttcctaa tagtttggac
     5641 cagacagaca tgcacggaga ctcagaatac aacatcatgt ttggtcccga catctgtggc
     5701 cctggcacca agaaggttca tgtcatcttc aactacaagg gcaagaacgt gctgatcaac
     5761 aaggacatcc gttgcaagga tgatgagttt acacacctgt acacactgat tgtgcggcca
     5821 gacaacacct atgaggtgaa gattgacaac agccaggtgg agtccggctc cttggaagac
     5881 gattgggact tcctgccacc caagaagata aaggatcctg atgcttcaaa accggaagac
     5941 tgggatgagc gggccaagat cgatgatccc acagactcca agcctgagga ctgggacaag
     6001 cccgagcata tccctgaccc tgatgctaag aagcccgagg actgggatga agagatggac
     6061 ggagagtggg aacccccagt gattcagaac cctgagtaca agggtgagtg gaagccccgg
     6121 cagatcgaca acccagatta caagggcact tggatccacc cagaaattga caaccccgag
     6181 tattctcccg atcccagtat ctatgcctat gataactttg gcgtgctggg cctggacctc
     6241 tggcaggtca agtctggcac catctttgac aacttcctca tcaccaacga tgaggcatac
     6301 gctgaggagt ttggcaacga gacgtggggc gtaacaaagg cagcagagaa acaaatgaag
     6361 gacaaacagg acgaggagca gaggcttaag gaggaggaag aagacaagaa acgcaaagag
     6421 gaggaggagg cagaggacaa ggaggatgat gaggacaaag atgaggatga ggaggatgag
     6481 gaggacaagg aggaagatga ggaggaagat gtccccggcc aggccaagga cgagctgtag
     6541 tctagataat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct
     6601 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc
     6661 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct
     6721 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga
     6781 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg
     6841 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct
     6901 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg
     6961 ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc
     7021 gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagcccaag cttaccatga
     7081 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     7141 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     7201 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     7261 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     7321 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     7381 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     7441 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     7501 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     7561 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     7621 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgaccgc
     7681 gtctggaaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     7741 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     7801 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     7861 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     7921 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     7981 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     8041 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     8101 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     8161 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     8221 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     8281 ggaattaatt ctgcagtcga gacctagaaa aacatggagc aatcacaagt agcaatacag
     8341 cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc
     8401 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     8461 actttttaaa agaaaagagg ggactggaag ggctaattca ctcccaacga agacaagata
     8521 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     8581 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     8641 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     8701 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     8761 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     8821 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     8881 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     8941 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     9001 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     9061 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     9121 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     9181 agtgagaggc cttgacattg ctagcgtttt accgtcgacc tctagctaga gcttggcgta
     9241 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     9301 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     9361 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     9421 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     9481 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     9541 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     9601 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     9661 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     9721 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     9781 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     9841 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     9901 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     9961 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
    10021 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
    10081 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
    10141 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    10201 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    10261 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
    10321 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
    10381 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
    10441 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    10501 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    10561 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    10621 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    10681 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    10741 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    10801 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    10861 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    10921 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    10981 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    11041 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    11101 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    11161 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    11221 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    11281 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    11341 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    11401 cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac aaataaagca
    11461 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    11521 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa
    11581 atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt
    11641 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa
    11701 tatgtactac aaacttagta gtttttaaag aaattgtatt tgttaaatat gtactacaaa
    11761 cttagtagt
//
