LOCUS       V026954                12262 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026954
VERSION     V026954
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2826..6305
                     /label="Itgal(NM_001033998)"
                     /note="Itgal(NM_001033998)"
                     /gene="Itgal"
     CDS             6336..6992
                     /codon_start=1
                     /gene=""
                     /product="glutathione S-transferase from Schistosoma
                     japonicum"
                     /transl_table=1
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK*"
                     /label="GST"
     promoter        7050..7549
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             7570..8169
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8183..8771
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8978..9611
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(9740..9756)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9764..9780
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9788..9818)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9833..9854
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10142..10730)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10901..11761)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11762..11866)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    11914..12048
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ccaccatgag tttccggatc cctggcccca gacttctgct attggggctc cagctgtttg
     2881 tgaatgcctg gagctacaac ctggacacgc ggcatgcgca gagcttcttg acacaagctg
     2941 gaagacattt tgggtaccag gtcttgcagt ttggagatgg ggttgtcgtg ggagctccag
     3001 gagaggggaa cagcacagga aacctctatc actgtcaccc aagcagcggc tcctgccagc
     3061 cagtccacct aggttctaac catacctcca aatacttggg aatgaccatg gtgacagatg
     3121 ctgccaaggg aagccttttg gcctgtgatc ctggactgtc tcggacctgt gatcagaacc
     3181 cctacctcag tgggctgtgc tacttatttt cccagagcct ggggaaacct atattacaaa
     3241 atcgtcctgc ttatcaggaa tgtatgaagg gcaatgtcga cctagtgttt ctgttcgatg
     3301 gctcacagag cttggataaa aaggactttg aaaaaatcgt ggacttcatg aaggatgtga
     3361 tgaggaaact cagcaacact tcctaccagt ttgctgctgt tcagttttcc acagagtgca
     3421 aaacagaatt tactttcttg gactacatca agctaaacaa gaatcctgat gttctgctag
     3481 gcaacgtgac gcccatgttc ttgctgacca ataccttccg tgccatcaac tacgtggtga
     3541 cacacgtgtt caaagaagag tctggtgcca ggccagatgc taccaaggtg cttgtcatca
     3601 ttacagacgg ggaagcaagt gacaatggca acatcgatgc agcccaagac ataacccgct
     3661 atatcatcgg gattggcaag catttttcaa ccacacaaaa gcaggagaaa ctccacatat
     3721 ttgcctcaaa acctgtagag gaatttgtca agattctgga cacatttgag aagctgaagg
     3781 atctgtttac tgatctgcag aggaagattt atgccattga gggcacaagc aaacaggacc
     3841 tggcatcctt taatatggaa ctctcctcca gcgggatcag cgcagacctc agcaagggcc
     3901 atgcggttgt tggagccgtt ggggctaagg attgggctgg gggctttctg gacctgcatg
     3961 aagacctgga gggtgccaca tttgttgggc aggaaccgct gacctcagat gagagaggag
     4021 gttatctggg ttacactgtg gcctggctga cctcccggag ctctagacca ctgctggcag
     4081 caggagctcc gcggtaccag catgtgggac aagtgctgct tttccaagcc ccagaagctg
     4141 gaggacattg gaaccagact cagaagatag aagggactca gatcggatct tactttggtg
     4201 gggagctatg tagcgttgac ctgcaccaag atggcgagac agatctgctg ctgattggag
     4261 cgcccctctt ctacggggag cagagaggag gccgagtgtc cgtttaccag agaagacggt
     4321 cactgttcga aatggtctca gagctgcagg gtgacccagg ctacccgctt ggtcggtttg
     4381 gagctgccat aagtgccctg acagacatca atggggatgg gctgactgat gtggccgtgg
     4441 gagcccctct ggaggagcag ggggctgtgt atatcttcaa tgggaagcct ggtgggttca
     4501 gttcccagtc aagtcagcgt ataccaggaa cccaggtgtc cccgggagtc cggtggtttg
     4561 gacgctccat ccatggggtg aaggaccttg gaggggacag gctggcagat gtggttgtag
     4621 gagctgaggg ccaggtgatt gtgctgagct cgaggccggt ggtggatatt gtcactgagc
     4681 tgtccttctc cccagacgaa atcccagtgc atgaggtgga gtgctcctac tcagccagtc
     4741 aggagcagaa ggaaggcgtc aagctcaagg tgtgcttcca gatccgcccc cttacgtctc
     4801 agtttcaagg tcgcctgctt gccaacctca gctacaccct gcagctggac ggccatcgga
     4861 cgaggagccg aggtctgttc ccaggaggaa gccgtgagct cggcggaaac acatttgtca
     4921 ccccagatag gtcctgcatg gacttccact tccacttccc aatctgcatt caagacctca
     4981 tctcccctat caatgtctct ctgaatttct ctcttttgga ggaagaagga tcaccaaggg
     5041 accaaaaggg gggcagggac atgcagccca tcctgagacc ttcgatccat gcagtcacta
     5101 aggagatccc ctttgagaag aactgtggtg aggataagaa gtgtgaggca gacctggccc
     5161 tgtcacctcc tgccagatcc ggagtcctgc gtctgatgtc ctctgccagc cttgctgtgg
     5221 agtggacact gaggaacttg ggagaagacg cttactgggt gcgactggac ctggacttcc
     5281 cccggggcct ctccttccga aaagtggaga tgcttcagcc acacagccaa atacctgtga
     5341 gctgcgagga gctcaccgag gagtcgagtc tcctgactaa gaccctgaaa tgcaatgtga
     5401 gctctcccat cttcaaagca ggcaaacaga tgaccctcca ggtgatgttt aacacgctac
     5461 tcaacagttc ctggggagat tttgtcgagc tgaacggcac tgtgcactgt gagaacgaga
     5521 actcaagcct cgggaaggac aactcagcca ccacccgcat tcctgtcctg taccctgtca
     5581 acatccttac tgaggaccag gagaactcca cactctatat cagtttcacc cctaaaggtc
     5641 ccaagactca acaagtccag catatctaca aggtgaggat tcagccttct gcctatgacc
     5701 acaacatgcc cgcactggag gccttggttg aggtgccgca gcctcacagc gagggcccca
     5761 tcgcacacac atggactgtg catacggatc cccttgtcac ttgccactgt gaggacctga
     5821 agaagccaac cagggaagct gagccctgtc tgcctggagt ccagttccgc tgcccaattg
     5881 tcttcaggca agagaccttc atccgagtgg ccgggactgt ggaactctca gagaaaatca
     5941 aggcctcctc cacactcagc ctctgcagct cgctctcagt ctccttcaac agcagcaagc
     6001 atttccattt gtatggtagc aaggcctcca aggcccaggt cctcatgaag gttgacatga
     6061 tctatgagaa ggagatgctt cacctgtacg tgctcagcgg cattgggggg ctattgctac
     6121 tgtccctgat tttcctagcg ctctacaagg ttggtttctt caaacggaag ctgaaggaga
     6181 agatggaggc ggatggaggt gttccaaatg gaagccctgg cgaagacgct gaccctctgg
     6241 cagtacctgg ggaagagacc aaagatatgg gctgtctaga gcccctccgt gagagtgaca
     6301 aggacggtgg aggcggatca ggaggcggtg gatctatgtc ccctatacta ggttattgga
     6361 aaattaaggg ccttgtgcaa cccactcgac ttcttttgga atatcttgaa gaaaaatatg
     6421 aagagcattt gtatgagcgc gatgaaggtg ataaatggcg aaacaaaaag tttgaattgg
     6481 gtttggagtt tcccaatctt ccttattata ttgatggtga tgttaaatta acacagtcta
     6541 tggccatcat acgttatata gctgacaagc acaacatgtt gggtggttgt ccaaaagagc
     6601 gtgcagagat ttcaatgctt gaaggagcgg ttttggatat tagatacggt gtttcgagaa
     6661 ttgcatatag taaagacttt gaaactctca aagttgattt tcttagcaag ctacctgaaa
     6721 tgctgaaaat gttcgaagat cgtttatgtc ataaaacata tttaaatggt gatcatgtaa
     6781 cccatcctga cttcatgttg tatgacgctc ttgatgttgt tttatacatg gacccaatgt
     6841 gcctggatgc gttcccaaaa ttagtttgtt ttaaaaaacg tattgaagct atcccacaaa
     6901 ttgataagta cttgaaatcc agcaagtata tagcatggcc tttgcagggc tggcaagcca
     6961 cgtttggtgg tggcgaccat cctccaaaat gaattctgca gtcgacggta ccgcgggccc
     7021 gggatcccgc gactctagat aattctaccg ggtaggggag gcgcttttcc caaggcagtc
     7081 tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc
     7141 tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt
     7201 cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc
     7261 gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac
     7321 cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt
     7381 cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct
     7441 caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa
     7501 gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc
     7561 aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     7621 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     7681 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     7741 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     7801 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     7861 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     7921 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     7981 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     8041 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     8101 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     8161 ggtgcctgac cgcgtctgga acaatcaacc tctggattac aaaatttgtg aaagattgac
     8221 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     8281 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     8341 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     8401 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     8461 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     8521 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     8581 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     8641 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     8701 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     8761 cgcctccccg cctggaatta attctgcagt cgagacctag aaaaacatgg agcaatcaca
     8821 agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag
     8881 gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct
     8941 gtagatctta gccacttttt aaaagaaaag aggggactgg aagggctaat tcactcccaa
     9001 cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattag
     9061 cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag
     9121 ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg
     9181 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg
     9241 tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag
     9301 aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt
     9361 ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt
     9421 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     9481 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     9541 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     9601 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     9661 atgaatatca gagagtgaga ggccttgaca ttgctagcgt tttaccgtcg acctctagct
     9721 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     9781 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     9841 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     9901 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     9961 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
    10021 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
    10081 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
    10141 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
    10201 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
    10261 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
    10321 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
    10381 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
    10441 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
    10501 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
    10561 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
    10621 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
    10681 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
    10741 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
    10801 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
    10861 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
    10921 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
    10981 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
    11041 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
    11101 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
    11161 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
    11221 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    11281 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    11341 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    11401 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    11461 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    11521 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    11581 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    11641 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    11701 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    11761 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    11821 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    11881 aagtgccacc tgacgtcgac ggatcgggag atcaacttgt ttattgcagc ttataatggt
    11941 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
    12001 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact
    12061 caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac
    12121 ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat
    12181 tgtatttgtt aaatatgtac tacaaactta gtagttttta aagaaattgt atttgttaaa
    12241 tatgtactac aaacttagta gt
//
