LOCUS V020510 9295 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V020510 VERSION V020510 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2826..4025 /label="MAP2K2(NM_030662)" /note="MAP2K2(NM_030662)" /gene="MAP2K2" CDS 4026..4052 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 4083..4582 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 4603..5202 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5216..5804 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6011..6644 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(6773..6789) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6797..6813 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6821..6851) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6866..6887 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7175..7763) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7934..8794) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8795..8899) /gene="bla" /label="AmpR promoter" polyA_signal 8947..9081 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ccaccatgct ggcccggagg aagccggtgc tgccggcgct caccatcaac cctaccatcg 2881 ccgagggccc atcccctacc agcgagggcg cctccgaggc aaacctggtg gacctgcaga 2941 agaagctgga ggagctggaa cttgacgagc agcagaagaa gcggctggaa gcctttctca 3001 cccagaaagc caaggtcggc gaactcaaag acgatgactt cgaaaggatc tcagagctgg 3061 gcgcgggcaa cggcggggtg gtcaccaaag tccagcacag accctcgggc ctcatcatgg 3121 ccaggaagct gatccacctt gagatcaagc cggccatccg gaaccagatc atccgcgagc 3181 tgcaggtcct gcacgaatgc aactcgccgt acatcgtggg cttctacggg gccttctaca 3241 gtgacgggga gatcagcatt tgcatggaac acatggacgg cggctccctg gaccaggtgc 3301 tgaaagaggc caagaggatt cccgaggaga tcctggggaa agtcagcatc gcggttctcc 3361 ggggcttggc gtacctccga gagaagcacc agatcatgca ccgagatgtg aagccctcca 3421 acatcctcgt gaactctaga ggggagatca agctgtgtga cttcggggtg agcggccagc 3481 tcatcgactc catggccaac tccttcgtgg gcacgcgctc ctacatggct ccggagcggt 3541 tgcagggcac acattactcg gtgcagtcgg acatctggag catgggcctg tccctggtgg 3601 agctggccgt cggaaggtac cccatccccc cgcccgacgc caaagagctg gaggccatct 3661 ttggccggcc cgtggtcgac ggggaagaag gagagcctca cagcatctcg cctcggccga 3721 ggccccccgg gcgccccgtc agcggtcacg ggatggatag ccggcctgcc atggccatct 3781 ttgaactcct ggactatatt gtgaacgagc cacctcctaa gctgcccaac ggtgtgttca 3841 cccccgactt ccaggagttt gtcaataaat gcctcatcaa gaacccagcg gagcgggcgg 3901 acctgaagat gctcacaaac cacaccttca tcaagcggtc cgaggtggaa gaagtggatt 3961 ttgccggctg gttgtgtaaa accctgcggc tgaaccagcc cggcacaccc acgcgcaccg 4021 ccgtgtaccc atacgacgta ccagattacg cttaggatcc cgcgactcta gataattcta 4081 ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct 4141 gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag 4201 gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt 4261 caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc 4321 acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct 4381 ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag 4441 gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc 4501 ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc 4561 ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgaccga gtacaagccc 4621 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg 4681 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc 4741 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc 4801 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg 4861 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag 4921 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc 4981 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc 5041 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc 5101 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa 5161 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaccgcgtct ggaacaatca 5221 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt 5281 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc 5341 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc 5401 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg 5461 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc 5521 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg 5581 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg 5641 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc 5701 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct 5761 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttaattctgc 5821 agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct 5881 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag 5941 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa 6001 aagaggggac tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg 6061 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc 6121 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta 6181 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg 6241 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac 6301 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag 6361 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga 6421 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga 6481 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata 6541 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta 6601 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca 6661 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggccttg 6721 acattgctag cgttttaccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc 6781 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 6841 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 6901 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 6961 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 7021 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 7081 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 7141 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 7201 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 7261 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 7321 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 7381 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 7441 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 7501 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 7561 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 7621 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 7681 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 7741 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 7801 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 7861 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 7921 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 7981 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 8041 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 8101 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 8161 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 8221 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 8281 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 8341 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 8401 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 8461 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 8521 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 8581 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 8641 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 8701 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 8761 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 8821 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 8881 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg 8941 gagatcaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 9001 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 9061 gtatcttatc atgtctggat caactggata actcaagcta accaaaatca tcccaaactt 9121 cccaccccat accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt 9181 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac 9241 ttagtagttt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagt //