LOCUS V045925 9784 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V045925 VERSION V045925 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2843..4519 /label="MLLT1(NM_005934)" /note="MLLT1(NM_005934)" /gene="MLLT1" CDS 4526..4552 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 4572..5071 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 5092..5691 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5705..6293 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6500..7133 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(7262..7278) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7286..7302 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7310..7340) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7355..7376 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7664..8252) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8423..9283) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9284..9388) /gene="bla" /label="AmpR promoter" polyA_signal 9436..9570 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcgcca ccatggacaa tcagtgcacc gtccaggtga ggttagagct 2881 ggggcatcgc gcccaactgc gcaagaagcc caccacggag gggttcactc acgactggat 2941 ggtgtttgtc cgcggccccg agcaatgtga catccagcac ttcgtggaga aggtggtctt 3001 ctggctgcac gacagcttcc ccaagcccag acgcgtgtgc aaggagcccc cctacaaagt 3061 agaggagtcg gggtacgctg gcttcatcat gcccatcgag gtgcacttca aaaacaagga 3121 ggagccgagg aaggtctgct tcacctacga cctgttcctg aacctggaag gcaacccgcc 3181 cgtgaaccac ctgcgctgcg agaagctcac cttcaacaac cccaccacgg agttccggta 3241 caagctcctg cgggccggcg gggtgatggt aatgcccgaa ggagcagaca cggtgtccag 3301 gcccagtccc gactacccca tgttacccac aattccactc tctgccttct ctgaccccaa 3361 gaagaccaaa ccatcccacg gctccaagga cgccaacaag gagagcagca agacctccaa 3421 gccacacaag gtgaccaagg agcaccggga gcggccccgc aaagactccg agagcaagag 3481 ctcctccaag gagctggagc gtgagcaggc caaaagctcc aaggacacct cgcggaagct 3541 gggcgagggc cggctgccca aggaggagaa ggcgccaccg cccaaggctg ccttcaagga 3601 acccaagatg gccctgaaag agaccaagct ggaaagcact tctccgaaag gtggtccacc 3661 tcctccacct ccaccacctc ctcgtgcttc ttctaagcgg ccggccaccg ccgactcgcc 3721 aaagcccagc gccaagaagc agaagaagag cagctcgaag gggtcccgga gtgctccagg 3781 cacctcgccc cgcacctcct cctcctcctc cttctcggac aagaagccgg ccaaggacaa 3841 gagcagcacc agaggggaga aggtgaaggc cgagagtgag ccccgggagg ccaaaaaggc 3901 cctggaggtg gaggagtcca actcagagga cgaggcctcc ttcaagtccg agtctgccca 3961 gtcaagcccg tccaactcca gctccagctc agactccagc tcagactcag acttcgagcc 4021 atcccagaac cacagccaag gacccctgcg ctccatggtg gaggacctgc agtccgagga 4081 gtccgacgag gacgactctt cgtcaggcga ggaggctgcc ggcaagacca acccggggag 4141 ggactccagg ttgagcttca gcgacagcga gagtgacaac agcgccgact cctccctgcc 4201 cagccgtgag cccccacccc cccagaagcc acccccgccc aacagcaagg tgtcaggccg 4261 gaggagcccc gagtcctgca gcaagcctga gaagatcctc aagaagggca cctacgacaa 4321 ggcctacacg gatgagctgg tggagctaca ccggaggctg atggcgctgc gggagcgcaa 4381 cgtgctgcag cagattgtga atctgatcga ggagactggc cacttcaatg tcaccaacac 4441 caccttcgac ttcgacctct tctccctgga cgagaccacc gtgcgcaaac tgcagagctg 4501 cctggaggcc gtggccacag gatcctaccc atacgacgta ccagattacg cttagtctag 4561 ataattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc 4621 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc 4681 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc 4741 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat 4801 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg 4861 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag 4921 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc 4981 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct 5041 gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac catgaccgag 5101 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc 5161 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc 5221 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag 5281 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa 5341 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg 5401 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg 5461 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc 5521 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc 5581 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag 5641 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg accgcgtctg 5701 gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt 5761 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc 5821 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga 5881 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc 5941 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct 6001 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg 6061 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct 6121 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc 6181 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg 6241 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat 6301 taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct 6361 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc 6421 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt 6481 ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca agatatcctt 6541 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg 6601 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca 6661 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg 6721 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca 6781 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct 6841 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg 6901 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct 6961 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 7021 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 7081 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag 7141 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga 7201 gaggccttga cattgctagc gttttaccgt cgacctctag ctagagcttg gcgtaatcat 7261 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 7321 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 7381 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 7441 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 7501 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 7561 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 7621 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 7681 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 7741 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 7801 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 7861 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 7921 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 7981 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 8041 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 8101 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 8161 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 8221 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 8281 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 8341 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 8401 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 8461 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 8521 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 8581 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 8641 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 8701 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 8761 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 8821 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 8881 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 8941 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 9001 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 9061 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 9121 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 9181 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 9241 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 9301 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 9361 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg 9421 acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc 9481 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 9541 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat 9601 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc 9661 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt 9721 actacaaact tagtagtttt taaagaaatt gtatttgtta aatatgtact acaaacttag 9781 tagt //