LOCUS       V044850                10111 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044850
VERSION     V044850
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2843..3496
                     /codon_start=1
                     /gene=""
                     /product="glutathione S-transferase from Schistosoma
                     japonicum"
                     /transl_table=1
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
                     /label="GST"
     CDS             3518..4879
                     /label="MYC(NM_002467)"
                     /note="MYC(NM_002467)"
                     /gene="MYC"
     promoter        4899..5398
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             5419..6018
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6032..6620
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6827..7460
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(7589..7605)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7613..7629
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7637..7667)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7682..7703
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7991..8579)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8750..9610)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9611..9715)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    9763..9897
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattcgcca ccatgtcccc tatactaggt tattggaaaa ttaagggcct
     2881 tgtgcaaccc actcgacttc ttttggaata tcttgaagaa aaatatgaag agcatttgta
     2941 tgagcgcgat gaaggtgata aatggcgaaa caaaaagttt gaattgggtt tggagtttcc
     3001 caatcttcct tattatattg atggtgatgt taaattaaca cagtctatgg ccatcatacg
     3061 ttatatagct gacaagcaca acatgttggg tggttgtcca aaagagcgtg cagagatttc
     3121 aatgcttgaa ggagcggttt tggatattag atacggtgtt tcgagaattg catatagtaa
     3181 agactttgaa actctcaaag ttgattttct tagcaagcta cctgaaatgc tgaaaatgtt
     3241 cgaagatcgt ttatgtcata aaacatattt aaatggtgat catgtaaccc atcctgactt
     3301 catgttgtat gacgctcttg atgttgtttt atacatggac ccaatgtgcc tggatgcgtt
     3361 cccaaaatta gtttgtttta aaaaacgtat tgaagctatc ccacaaattg ataagtactt
     3421 gaaatccagc aagtatatag catggccttt gcagggctgg caagccacgt ttggtggtgg
     3481 cgaccatcct ccaaaaggat ccggtggagg cggatcactg gatttttttc gggtagtgga
     3541 aaaccagcag cctcccgcga cgatgcccct caacgttagc ttcaccaaca ggaactatga
     3601 cctcgactac gactcggtgc agccgtattt ctactgcgac gaggaggaga acttctacca
     3661 gcagcagcag cagagcgagc tgcagccccc ggcgcccagc gaggatatct ggaagaaatt
     3721 cgagctgctg cccaccccgc ccctgtcccc tagccgccgc tccgggctct gctcgccctc
     3781 ctacgttgcg gtcacaccct tctcccttcg gggagacaac gacggcggtg gcgggagctt
     3841 ctccacggcc gaccagctgg agatggtgac cgagctgctg ggaggagaca tggtgaacca
     3901 gagtttcatc tgcgacccgg acgacgagac cttcatcaaa aacatcatca tccaggactg
     3961 tatgtggagc ggcttctcgg ccgccgccaa gctcgtctca gagaagctgg cctcctacca
     4021 ggctgcgcgc aaagacagcg gcagcccgaa ccccgcccgc ggccacagcg tctgctccac
     4081 ctccagcttg tacctgcagg atctgagcgc cgccgcctca gagtgcatcg acccctcggt
     4141 ggtcttcccc taccctctca acgacagcag ctcgcccaag tcctgcgcct cgcaagactc
     4201 cagcgccttc tctccgtcct cggattctct gctctcctcg acggagtcct ccccgcaggg
     4261 cagccccgag cccctggtgc tccatgagga gacaccgccc accaccagca gcgactctga
     4321 ggaggaacaa gaagatgagg aagaaatcga tgttgtttct gtggaaaaga ggcaggctcc
     4381 tggcaaaagg tcagagtctg gatcaccttc tgctggaggc cacagcaaac ctcctcacag
     4441 cccactggtc ctcaagaggt gccacgtctc cacacatcag cacaactacg cagcgcctcc
     4501 ctccactcgg aaggactatc ctgctgccaa gagggtcaag ttggacagtg tcagagtcct
     4561 gagacagatc agcaacaacc gaaaatgcac cagccccagg tcctcggaca ccgaggagaa
     4621 tgtcaagagg cgaacacaca acgtcttgga gcgccagagg aggaacgagc taaaacggag
     4681 cttttttgcc ctgcgtgacc agatcccgga gttggaaaac aatgaaaagg cccccaaggt
     4741 agttatcctt aaaaaagcca cagcatacat cctgtccgtc caagcagagg agcaaaagct
     4801 catttctgaa gaggacttgt tgcggaaacg acgagaacag ttgaaacaca aacttgaaca
     4861 gctacggaac tcttgtgcgt aatctagata attctaccgg gtaggggagg cgcttttccc
     4921 aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg
     4981 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg
     5041 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca
     5101 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat
     5161 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct
     5221 ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag
     5281 gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac
     5341 gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac
     5401 ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     5461 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     5521 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     5581 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     5641 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     5701 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     5761 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     5821 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     5881 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     5941 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     6001 cgcaagcccg gtgcctgacc gcgtctggaa caatcaacct ctggattaca aaatttgtga
     6061 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     6121 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     6181 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     6241 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     6301 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     6361 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     6421 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     6481 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     6541 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     6601 cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga
     6661 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     6721 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     6781 aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt
     6841 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     6901 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
     6961 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
     7021 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
     7081 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
     7141 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
     7201 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
     7261 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     7321 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7381 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7441 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     7501 tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt ttaccgtcga
     7561 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     7621 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     7681 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     7741 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     7801 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     7861 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     7921 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7981 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     8041 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     8101 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     8161 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8221 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     8281 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8341 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8401 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     8461 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8521 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8581 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     8641 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     8701 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     8761 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     8821 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     8881 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     8941 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     9001 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     9061 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     9121 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     9181 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     9241 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     9301 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     9361 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     9421 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     9481 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     9541 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     9601 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     9661 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     9721 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt tattgcagct
     9781 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     9841 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
     9901 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
     9961 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
    10021 taaagaaatt gtatttgtta aatatgtact acaaacttag tagtttttaa agaaattgta
    10081 tttgttaaat atgtactaca aacttagtag t
//
