LOCUS V021435 10352 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V021435 VERSION V021435 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2826..5087 /label="NBN(NM_002485)" /note="NBN(NM_002485)" /gene="NBN" CDS 5094..5120 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 5140..5639 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 5660..6259 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 6273..6861 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 7068..7701 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(7830..7846) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7854..7870 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7878..7908) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7923..7944 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8232..8820) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8991..9851) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9852..9956) /gene="bla" /label="AmpR promoter" polyA_signal 10004..10138 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ccaccatgtg gaaactgctg cccgccgcgg gcccggcagg aggagaacca tacagacttt 2881 tgactggcgt tgagtacgtt gttggaagga aaaactgtgc cattctgatt gaaaatgatc 2941 agtcgatcag ccgaaatcat gctgtgttaa ctgctaactt ttctgtaacc aacctgagtc 3001 aaacagatga aatccctgta ttgacattaa aagataattc taagtatggt acctttgtta 3061 atgaggaaaa aatgcagaat ggcttttccc gaactttgaa gtcgggggat ggtattactt 3121 ttggagtgtt tggaagtaaa ttcagaatag agtatgagcc tttggttgca tgctcttctt 3181 gtttagatgt ctctgggaaa actgctttaa atcaagctat attgcaactt ggaggattta 3241 ctgtaaacaa ttggacagaa gaatgcactc accttgtcat ggtatcagtg aaagttacca 3301 ttaaaacaat atgtgcactc atttgtggac gtccaattgt aaagccagaa tattttactg 3361 aattcctgaa agcagttgag tccaagaagc agcctccaca aattgaaagt ttttacccac 3421 ctcttgatga accatctatt ggaagtaaaa atgttgatct gtcaggacgg caggaaagaa 3481 aacaaatctt caaagggaaa acatttatat ttttgaatgc caaacagcat aagaaattga 3541 gttccgcagt tgtctttgga ggtggggaag ctaggttgat aacagaagag aatgaagaag 3601 aacataattt ctttttggct ccgggaacgt gtgttgttga tacaggaata acaaactcac 3661 agaccttaat tcctgactgt cagaagaaat ggattcagtc aataatggat atgctccaaa 3721 ggcaaggtct tagacctatt cctgaagcag aaattggatt ggcggtgatt ttcatgacta 3781 caaagaatta ctgtgatcct cagggccatc ccagtacagg attaaagaca acaactccag 3841 gaccaagcct ttcacaaggc gtgtcagttg atgaaaaact aatgccaagc gccccagtga 3901 acactacaac atacgtagct gacacagaat cagagcaagc agatacatgg gatttgagtg 3961 aaaggccaaa agaaatcaaa gtctccaaaa tggaacaaaa attcagaatg ctttcacaag 4021 atgcacccac tgtaaaggag tcctgcaaaa caagctctaa taataatagt atggtatcaa 4081 atactttggc taagatgaga atcccaaact atcagctttc accaactaaa ttgccaagta 4141 taaataaaag taaagatagg gcttctcagc agcagcagac caactccatc agaaactact 4201 ttcagccgtc taccaaaaaa agggaaaggg atgaagaaaa tcaagaaatg tcttcatgca 4261 aatcagcaag aatagaaacg tcttgttctc ttttagaaca aacacaacct gctacaccct 4321 cattgtggaa aaataaggag cagcatctat ctgagaatga gcctgtggac acaaactcag 4381 acaataactt atttacagat acagatttaa aatctattgt gaaaaattct gccagtaaat 4441 ctcatgctgc agaaaagcta agatcaaata aaaaaaggga aatggatgat gtggccatag 4501 aagatgaagt attggaacag ttattcaagg acacaaaacc agagttagaa attgatgtga 4561 aagttcaaaa acaggaggaa gatgtcaatg ttagaaaaag gccaaggatg gatatagaaa 4621 caaatgacac tttcagtgat gaagcagtac cagaaagtag caaaatatct caagaaaatg 4681 aaattgggaa gaaacgtgaa ctcaaggaag actcactatg gtcagctaaa gaaatatcta 4741 acaatgacaa acttcaggat gatagtgaga tgcttccaaa aaagctgtta ttgactgaat 4801 ttagatcact ggtgattaaa aactctactt ccagaaatcc atctggcata aatgatgatt 4861 atggtcaact aaaaaatttc aagaaattca aaaaggtcac atatcctgga gcaggaaaac 4921 ttccacacat cattggagga tcagatctaa tagctcatca tgctcgaaag aatacagaac 4981 tagaagagtg gctaaggcag gaaatggagg tacaaaatca acatgcaaaa gaagagtctc 5041 ttgctgatga tctttttaga tacaatcctt atttaaaaag gagaagagga tcctacccat 5101 acgacgtacc agattacgct tagtctagat aattctaccg ggtaggggag gcgcttttcc 5161 caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg 5221 gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg 5281 gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc 5341 agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga 5401 tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc 5461 tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca 5521 ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca 5581 cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga 5641 cctgcagccc aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga 5701 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg 5761 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct 5821 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc 5881 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg 5941 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc 6001 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca 6061 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc 6121 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct 6181 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac 6241 ccgcaagccc ggtgcctgac cgcgtctgga acaatcaacc tctggattac aaaatttgtg 6301 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt 6361 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata 6421 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg 6481 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc 6541 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct 6601 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt 6661 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg 6721 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc 6781 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct 6841 ccctttgggc cgcctccccg cctggaatta attctgcagt cgagacctag aaaaacatgg 6901 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca 6961 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta 7021 caaggcagct gtagatctta gccacttttt aaaagaaaag aggggactgg aagggctaat 7081 tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt 7141 ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg 7201 gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa 7261 caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt 7321 agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga 7381 gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc 7441 agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc 7501 agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct 7561 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag 7621 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt 7681 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac 7741 ttgcaaagaa atgaatatca gagagtgaga ggccttgaca ttgctagcgt tttaccgtcg 7801 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat 7861 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc 7921 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 7981 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 8041 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 8101 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 8161 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 8221 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 8281 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 8341 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 8401 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 8461 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 8521 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 8581 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 8641 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg 8701 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 8761 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 8821 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 8881 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 8941 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 9001 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 9061 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 9121 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 9181 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 9241 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 9301 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 9361 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 9421 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 9481 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 9541 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 9601 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 9661 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 9721 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 9781 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 9841 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 9901 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 9961 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcaacttgt ttattgcagc 10021 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 10081 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa 10141 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac 10201 tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt 10261 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttttta aagaaattgt 10321 atttgttaaa tatgtactac aaacttagta gt //