LOCUS       V021567                10292 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021567
VERSION     V021567
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2826..5048
                     /label="Nfe2l1(NM_008686)"
                     /note="Nfe2l1(NM_008686)"
                     /gene="Nfe2l1"
     promoter        5080..5579
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             5600..6199
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6213..6801
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7008..7641
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(7770..7786)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7794..7810
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7818..7848)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7863..7884
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8172..8760)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8931..9791)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9792..9896)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    9944..10078
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ccaccatgct ttctctgaag aaatatttaa cggaaggact tctccagttc accatcctgc
     2881 tgagtctgat tggggttcgg gtggacgtgg atacttacct gacctcacag ctcccccctc
     2941 tccgggagat catcctgggg cccagctctg cctataccca gacccagttc cacaacctga
     3001 ggaatacctt ggatggctat gggatccacc ccaagagcat agacctggac aattacttca
     3061 ctgcccggcg gctccttagt caggtgaggg ccctggatag gttccaggtg cctaccactg
     3121 aggtcaatgc ttggctggtc caccgagacc cggaggggtc tgtctctggc agccagccca
     3181 actcaggcct cgccctcgag agttccagtg gcctccaaga tgtgacaggc ccagacaacg
     3241 gggtgagaga aagcgaaacg gagcagggat tcggtgaaga tttggaggac ctgggggctg
     3301 tagcccctcc tgtcagtgga gacttaacca aagaggatat agatctgatt gacatccttt
     3361 ggcgacagga tattgatctg ggggctgggc gtgaggtttt tgactacagt catcgccaga
     3421 aggagcagga tgtggataag gaactgcaag atggacgaga acgagaggac acctggtcag
     3481 gcgagggtgc ggaagctctg gcccgagacc tgctagtaga tggagagact ggggagagct
     3541 tccctgcaca gttcccagct gacgtttcca gcatcccaga agcagtgcct agtgagagtg
     3601 agtcccccgc ccttcagaac agccttctat ctcctcttct gacggggaca gaatcaccat
     3661 ttgatttgga acagcagtgg caagatctca tgtccatcat ggaaatgcag gctatggaag
     3721 taaatacatc agcaagtgag attctgtaca atgcccctcc tggagaccct cttagcacca
     3781 actacagcct tgcacccaac actcccatca atcagaatgt cagcctgcat caggcgtccc
     3841 tggggggctg cagtcaggac ttctccctct tcagccccga ggtggagagc ctgcctgtgg
     3901 ctagcagctc cacactgctt ccactcgtcc ccagcaactc caccagtctc aactccacct
     3961 ttggctctac caacctagca gggcttttct ttccatccca gctcaatggc acagccaatg
     4021 acacatcagg ccctgagcta cctgaccccc ttgggggcct gttagacgaa gctatgctgg
     4081 atgagatcag cctgatggac ctggccattg aggagggctt caacccggtg caggcttccc
     4141 agctcgaaga ggagtttgac tctgactcag gcctctcctt ggactccagc catagccctt
     4201 cctctctgag cagctctgaa gggagctctt cttcttcctc ctcctcctct tcctcttctg
     4261 cttcctcctc tgcctcttct tccttctctg aggagggtgc tgttggttac agctctgact
     4321 ctgagaccct agacctagaa gaggctgagg gtgcagtggg ctaccagccg gaatactcca
     4381 agttctgccg catgagctat caggatcctt ctcagctctc ttgccttccc tacttagagc
     4441 atgtgggcca caatcataca tacaatatgg cacccagtgc ccttgactct gctgatctac
     4501 caccacccag caccctcaag aaaggtagca aggaaaagca ggctgacttc ctggacaagc
     4561 agatgagccg agatgagcac agagcccgag ccatgaagat cccattcacc aatgacaaga
     4621 tcatcaacct gcctgtagaa gaattcaatg agctgctgtc caaataccag ctgagcgagg
     4681 cccagctcag cctcatccgg gatatccggc gccggggcaa aaacaagatg gctgcacaga
     4741 actgccgcaa gcgcaagttg gacaccatcc taaacctaga acgtgatgtg gaggacttgc
     4801 agcgagataa ggcccgattg cttcgagaaa aggtagagtt ccttcggtct ctgcgacaga
     4861 tgaagcagaa ggtccaaagc ttataccagg aggtgtttgg gcggctgcgg gatgagcatg
     4921 ggaggcccta ctcacccagt cagtatgccc ttcagtatgc tggggatggc agtgtcctcc
     4981 tcattcctcg cacgatggct gaccagcagg ctcggcgaca ggagagaaag ccaaaggacc
     5041 ggaggaagtg aggatcccgc gactctagat aattctaccg ggtaggggag gcgcttttcc
     5101 caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg
     5161 gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg
     5221 gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc
     5281 agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga
     5341 tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc
     5401 tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca
     5461 ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca
     5521 cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga
     5581 cctgcagccc aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     5641 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     5701 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     5761 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     5821 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     5881 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     5941 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     6001 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     6061 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     6121 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     6181 ccgcaagccc ggtgcctgac cgcgtctgga acaatcaacc tctggattac aaaatttgtg
     6241 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     6301 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     6361 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     6421 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     6481 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     6541 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     6601 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     6661 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     6721 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     6781 ccctttgggc cgcctccccg cctggaatta attctgcagt cgagacctag aaaaacatgg
     6841 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca
     6901 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta
     6961 caaggcagct gtagatctta gccacttttt aaaagaaaag aggggactgg aagggctaat
     7021 tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt
     7081 ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg
     7141 gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa
     7201 caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt
     7261 agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga
     7321 gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc
     7381 agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc
     7441 agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     7501 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     7561 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     7621 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     7681 ttgcaaagaa atgaatatca gagagtgaga ggccttgaca ttgctagcgt tttaccgtcg
     7741 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     7801 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     7861 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     7921 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     7981 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     8041 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     8101 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8161 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     8221 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8281 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8341 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8401 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8461 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8521 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8581 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     8641 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8701 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     8761 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     8821 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     8881 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     8941 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     9001 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     9061 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     9121 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     9181 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     9241 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     9301 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     9361 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     9421 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     9481 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     9541 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     9601 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     9661 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     9721 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     9781 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     9841 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     9901 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcaacttgt ttattgcagc
     9961 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
    10021 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
    10081 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
    10141 tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt
    10201 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttttta aagaaattgt
    10261 atttgttaaa tatgtactac aaacttagta gt
//
