LOCUS V021567 10292 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V021567 VERSION V021567 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2826..5048 /label="Nfe2l1(NM_008686)" /note="Nfe2l1(NM_008686)" /gene="Nfe2l1" promoter 5080..5579 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 5600..6199 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 6213..6801 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 7008..7641 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(7770..7786) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7794..7810 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7818..7848) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7863..7884 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8172..8760) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8931..9791) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9792..9896) /gene="bla" /label="AmpR promoter" polyA_signal 9944..10078 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ccaccatgct ttctctgaag aaatatttaa cggaaggact tctccagttc accatcctgc 2881 tgagtctgat tggggttcgg gtggacgtgg atacttacct gacctcacag ctcccccctc 2941 tccgggagat catcctgggg cccagctctg cctataccca gacccagttc cacaacctga 3001 ggaatacctt ggatggctat gggatccacc ccaagagcat agacctggac aattacttca 3061 ctgcccggcg gctccttagt caggtgaggg ccctggatag gttccaggtg cctaccactg 3121 aggtcaatgc ttggctggtc caccgagacc cggaggggtc tgtctctggc agccagccca 3181 actcaggcct cgccctcgag agttccagtg gcctccaaga tgtgacaggc ccagacaacg 3241 gggtgagaga aagcgaaacg gagcagggat tcggtgaaga tttggaggac ctgggggctg 3301 tagcccctcc tgtcagtgga gacttaacca aagaggatat agatctgatt gacatccttt 3361 ggcgacagga tattgatctg ggggctgggc gtgaggtttt tgactacagt catcgccaga 3421 aggagcagga tgtggataag gaactgcaag atggacgaga acgagaggac acctggtcag 3481 gcgagggtgc ggaagctctg gcccgagacc tgctagtaga tggagagact ggggagagct 3541 tccctgcaca gttcccagct gacgtttcca gcatcccaga agcagtgcct agtgagagtg 3601 agtcccccgc ccttcagaac agccttctat ctcctcttct gacggggaca gaatcaccat 3661 ttgatttgga acagcagtgg caagatctca tgtccatcat ggaaatgcag gctatggaag 3721 taaatacatc agcaagtgag attctgtaca atgcccctcc tggagaccct cttagcacca 3781 actacagcct tgcacccaac actcccatca atcagaatgt cagcctgcat caggcgtccc 3841 tggggggctg cagtcaggac ttctccctct tcagccccga ggtggagagc ctgcctgtgg 3901 ctagcagctc cacactgctt ccactcgtcc ccagcaactc caccagtctc aactccacct 3961 ttggctctac caacctagca gggcttttct ttccatccca gctcaatggc acagccaatg 4021 acacatcagg ccctgagcta cctgaccccc ttgggggcct gttagacgaa gctatgctgg 4081 atgagatcag cctgatggac ctggccattg aggagggctt caacccggtg caggcttccc 4141 agctcgaaga ggagtttgac tctgactcag gcctctcctt ggactccagc catagccctt 4201 cctctctgag cagctctgaa gggagctctt cttcttcctc ctcctcctct tcctcttctg 4261 cttcctcctc tgcctcttct tccttctctg aggagggtgc tgttggttac agctctgact 4321 ctgagaccct agacctagaa gaggctgagg gtgcagtggg ctaccagccg gaatactcca 4381 agttctgccg catgagctat caggatcctt ctcagctctc ttgccttccc tacttagagc 4441 atgtgggcca caatcataca tacaatatgg cacccagtgc ccttgactct gctgatctac 4501 caccacccag caccctcaag aaaggtagca aggaaaagca ggctgacttc ctggacaagc 4561 agatgagccg agatgagcac agagcccgag ccatgaagat cccattcacc aatgacaaga 4621 tcatcaacct gcctgtagaa gaattcaatg agctgctgtc caaataccag ctgagcgagg 4681 cccagctcag cctcatccgg gatatccggc gccggggcaa aaacaagatg gctgcacaga 4741 actgccgcaa gcgcaagttg gacaccatcc taaacctaga acgtgatgtg gaggacttgc 4801 agcgagataa ggcccgattg cttcgagaaa aggtagagtt ccttcggtct ctgcgacaga 4861 tgaagcagaa ggtccaaagc ttataccagg aggtgtttgg gcggctgcgg gatgagcatg 4921 ggaggcccta ctcacccagt cagtatgccc ttcagtatgc tggggatggc agtgtcctcc 4981 tcattcctcg cacgatggct gaccagcagg ctcggcgaca ggagagaaag ccaaaggacc 5041 ggaggaagtg aggatcccgc gactctagat aattctaccg ggtaggggag gcgcttttcc 5101 caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg 5161 gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg 5221 gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc 5281 agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga 5341 tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc 5401 tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca 5461 ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca 5521 cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga 5581 cctgcagccc aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga 5641 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg 5701 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct 5761 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc 5821 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg 5881 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc 5941 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca 6001 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc 6061 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct 6121 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac 6181 ccgcaagccc ggtgcctgac cgcgtctgga acaatcaacc tctggattac aaaatttgtg 6241 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt 6301 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata 6361 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg 6421 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc 6481 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct 6541 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt 6601 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg 6661 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc 6721 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct 6781 ccctttgggc cgcctccccg cctggaatta attctgcagt cgagacctag aaaaacatgg 6841 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca 6901 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta 6961 caaggcagct gtagatctta gccacttttt aaaagaaaag aggggactgg aagggctaat 7021 tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt 7081 ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg 7141 gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa 7201 caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt 7261 agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga 7321 gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc 7381 agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc 7441 agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct 7501 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag 7561 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt 7621 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac 7681 ttgcaaagaa atgaatatca gagagtgaga ggccttgaca ttgctagcgt tttaccgtcg 7741 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat 7801 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc 7861 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 7921 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 7981 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 8041 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 8101 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 8161 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 8221 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 8281 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 8341 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 8401 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 8461 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 8521 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 8581 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg 8641 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 8701 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 8761 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 8821 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 8881 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 8941 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 9001 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 9061 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 9121 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 9181 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 9241 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 9301 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 9361 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 9421 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 9481 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 9541 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 9601 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 9661 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 9721 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 9781 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 9841 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 9901 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcaacttgt ttattgcagc 9961 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 10021 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa 10081 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac 10141 tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt 10201 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttttta aagaaattgt 10261 atttgttaaa tatgtactac aaacttagta gt //