LOCUS       V040077                11874 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040077
VERSION     V040077
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2827..5964
                     /label="OGT(NM_181672)"
                     /note="OGT(NM_181672)"
                     /gene="OGT"
     CDS             5977..6633
                     /codon_start=1
                     /gene=""
                     /product="glutathione S-transferase from Schistosoma
                     japonicum"
                     /transl_table=1
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK*"
                     /label="GST"
     promoter        6662..7161
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             7182..7781
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7795..8383
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8590..9223
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(9352..9368)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9376..9392
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9400..9430)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9445..9466
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9754..10342)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10513..11373)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11374..11478)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    11526..11660
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 gccaccatgg cgtcttccgt gggcaacgtg gccgacagca cagaaccaac gaaacgtatg
     2881 ctttccttcc aagggttagc tgagttggca catcgagaat atcaggcagg agattttgag
     2941 gcagctgaga gacactgcat gcagctctgg agacaagagc cagacaatac tggtgtgctt
     3001 ttattacttt catctataca cttccagtgt cgaaggctgg acagatctgc tcactttagc
     3061 actctggcaa ttaaacagaa cccccttctg gcagaagctt attcgaattt ggggaatgtg
     3121 tacaaggaaa gagggcagtt gcaggaggca attgagcatt atcgacatgc attgcgtctc
     3181 aaacctgatt tcatcgatgg ttatattaac ctggcagccg ccttggtagc agcgggtgac
     3241 atggaagggg cagtacaagc ttacgtctct gctcttcagt acaatcctga tttgtactgt
     3301 gttcgcagtg acctggggaa cctgctcaaa gccctgggtc gcttggaaga agccaaggca
     3361 tgttatttga aagcaattga gacgcaaccg aactttgcag tagcttggag taatcttggc
     3421 tgtgttttca atgcacaagg ggaaatttgg cttgcaattc atcactttga aaaggctgtc
     3481 acccttgacc caaactttct ggatgcttat atcaatttag gaaatgtctt gaaagaggca
     3541 cgcatttttg acagagctgt ggcagcttat cttcgtgccc taagtttgag tccaaatcac
     3601 gcagtggtgc acggcaacct ggcttgtgta tactatgagc aaggcctgat agatctggca
     3661 atagacacct acaggcgggc tatcgaacta caaccacatt tccctgatgc ttactgcaac
     3721 ctagccaatg ctctcaaaga gaagggcagt gttgctgaag cagaagattg ttataataca
     3781 gctctccgtc tgtgtcccac ccatgcagac tctctgaata acctagccaa tatcaaacga
     3841 gaacagggaa acattgaaga ggcagttcgc ttgtatcgta aagcattaga agtcttccca
     3901 gagtttgctg ctgcccattc aaatttagca agtgtactgc agcagcaggg aaaactgcag
     3961 gaagctctga tgcattataa ggaggctatt cgaatcagtc ctacctttgc tgatgcctac
     4021 tctaatatgg gaaacactct aaaggagatg caggatgttc agggagcctt gcagtgttat
     4081 acgcgtgcca tccaaattaa tcctgcattt gcagatgcac atagcaatct ggcttccatt
     4141 cataaggatt cagggaatat tccagaagcc atagcttctt accgcacggc tctgaaactt
     4201 aagcctgatt ttcctgatgc ttattgtaac ttggctcatt gcctgcagat tgtctgtgat
     4261 tggacagact atgatgagcg aatgaagaag ttggtcagta ttgtggctga ccagttagag
     4321 aagaataggt tgccttctgt gcatcctcat catagtatgc tatatcctct ttctcatggc
     4381 ttcaggaagg ctattgctga gaggcacggc aacctgtgct tagataagat taatgttctt
     4441 cataaaccac catatgaaca tccaaaagac ttgaagctca gtgatggtcg gctgcgtgta
     4501 ggatatgtga gttccgactt tgggaatcat cctacttctc accttatgca gtctattcca
     4561 ggcatgcaca atcctgataa atttgaggtg ttctgttatg ccctgagccc agacgatggc
     4621 acaaacttcc gagtgaaggt gatggcagaa gccaatcatt tcattgatct ttctcagatt
     4681 ccatgcaatg gaaaagcagc tgatcgcatc catcaggatg gaattcatat ccttgtaaat
     4741 atgaatggct atactaaggg cgctcgaaat gagctttttg ctctcaggcc agctcctatt
     4801 caggcaatgt ggctgggata ccctgggacg agtggtgcgc ttttcatgga ttatattatc
     4861 actgatcagg aaacttcgcc agctgaagtt gctgagcagt attccgagaa attggcttat
     4921 atgccccaca ctttttttat tggtgatcat gctaatatgt tccctcacct gaagaaaaaa
     4981 gcagtcatcg attttaagtc caatgggcac atttatgaca atcggatagt tctgaatggc
     5041 atcgacctca aagcatttct tgatagtcta ccagatgtga aaattgtcaa gatgaagtgt
     5101 cctgatggag gagacaatgc agatagcagt aacacagctc ttaatatgcc tgttattcct
     5161 atgaatacta ttgcagaagc agttattgaa atgattaacc gaggacagat tcaaataaca
     5221 attaatggat tcagtattag caatggactg gcaactactc agatcaacaa taaggctgca
     5281 actggagagg aggttccccg taccattatt gtaaccaccc gttctcagta cgggttacca
     5341 gaagatgcca tcgtatactg taactttaat cagttgtata aaattgaccc ttctactttg
     5401 cagatgtggg caaacattct gaagcgtgtt cccaatagtg tactctggct gttgcgtttt
     5461 ccagcagtag gagaacctaa tattcaacag tatgcacaaa acatgggcct gccccagaac
     5521 cgtatcattt tttcacctgt tgctcctaaa gaggaacacg tcaggagagg ccagctggct
     5581 gatgtctgct tggacactcc actctgtaat gggcacacca cagggatgga tgtcctctgg
     5641 gcagggaccc ccatggtgac tatgccagga gagactcttg cttctcgagt tgcagcatcc
     5701 cagctcactt gcttaggttg tcttgagctt attgctaaaa acagacaaga atatgaagac
     5761 atagctgtga agctgggaac tgatctagaa tacctgaaga aagttcgtgg caaagtctgg
     5821 aagcaaagaa tatctagccc tctgttcaac accaaacaat acacaatgga actagagcgg
     5881 ctctatctac agatgtggga gcattatgca gctggcaaca aacctgacca catgattaag
     5941 cctgttgaag tcactgagtc agcagaattc gccaccatgt cccctatact aggttattgg
     6001 aaaattaagg gccttgtgca acccactcga cttcttttgg aatatcttga agaaaaatat
     6061 gaagagcatt tgtatgagcg cgatgaaggt gataaatggc gaaacaaaaa gtttgaattg
     6121 ggtttggagt ttcccaatct tccttattat attgatggtg atgttaaatt aacacagtct
     6181 atggccatca tacgttatat agctgacaag cacaacatgt tgggtggttg tccaaaagag
     6241 cgtgcagaga tttcaatgct tgaaggagcg gttttggata ttagatacgg tgtttcgaga
     6301 attgcatata gtaaagactt tgaaactctc aaagttgatt ttcttagcaa gctacctgaa
     6361 atgctgaaaa tgttcgaaga tcgtttatgt cataaaacat atttaaatgg tgatcatgta
     6421 acccatcctg acttcatgtt gtatgacgct cttgatgttg ttttatacat ggacccaatg
     6481 tgcctggatg cgttcccaaa attagtttgt tttaaaaaac gtattgaagc tatcccacaa
     6541 attgataagt acttgaaatc cagcaagtat atagcatggc ctttgcaggg ctggcaagcc
     6601 acgtttggtg gtggcgacca tcctccaaaa tgaggatccc gcgactctag ataattctac
     6661 cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg
     6721 ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg
     6781 cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc
     6841 aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca
     6901 cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt
     6961 tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg
     7021 ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc
     7081 tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct
     7141 tcctcatctc cgggcctttc gacctgcagc ccaagcttac catgaccgag tacaagccca
     7201 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     7261 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
     7321 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     7381 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     7441 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     7501 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     7561 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     7621 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     7681 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     7741 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg accgcgtctg gaacaatcaa
     7801 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     7861 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     7921 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     7981 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     8041 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     8101 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     8161 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     8221 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     8281 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     8341 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat taattctgca
     8401 gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg
     8461 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg
     8521 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     8581 agaggggact ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga
     8641 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
     8701 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
     8761 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
     8821 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
     8881 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
     8941 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
     9001 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
     9061 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     9121 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     9181 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     9241 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggccttga
     9301 cattgctagc gttttaccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     9361 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     9421 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     9481 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     9541 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     9601 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     9661 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     9721 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     9781 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     9841 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     9901 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     9961 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
    10021 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
    10081 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
    10141 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
    10201 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
    10261 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
    10321 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
    10381 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
    10441 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
    10501 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
    10561 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
    10621 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
    10681 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
    10741 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
    10801 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
    10861 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
    10921 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    10981 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    11041 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    11101 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    11161 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    11221 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    11281 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    11341 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    11401 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    11461 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
    11521 agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
    11581 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
    11641 tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc
    11701 ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg
    11761 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact
    11821 tagtagtttt taaagaaatt gtatttgtta aatatgtact acaaacttag tagt
//
