LOCUS       V043824                11218 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043824
VERSION     V043824
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2843..5974
                     /label="PIK3CD(NM_005026)"
                     /note="PIK3CD(NM_005026)"
                     /gene="PIK3CD"
     promoter        6006..6505
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             6526..7125
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7139..7727
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7934..8567
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8696..8712)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8720..8736
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8744..8774)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8789..8810
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9098..9686)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9857..10717)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10718..10822)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    10870..11004
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattcgcca ccatgccccc tggggtggac tgccccatgg aattctggac
     2881 caaggaggag aatcagagcg ttgtggttga cttcctgctg cccacagggg tctacctgaa
     2941 cttccctgtg tcccgcaatg ccaacctcag caccatcaag cagctgctgt ggcaccgcgc
     3001 ccagtatgag ccgctcttcc acatgctcag tggccccgag gcctatgtgt tcacctgcat
     3061 caaccagaca gcggagcagc aagagctgga ggacgagcaa cggcgtctgt gtgacgtgca
     3121 gcccttcctg cccgtcctgc gcctggtggc ccgtgagggc gaccgcgtga agaagctcat
     3181 caactcacag atcagcctcc tcatcggcaa aggcctccac gagtttgact ccttgtgcga
     3241 cccagaagtg aacgactttc gcgccaagat gtgccaattc tgcgaggagg cggccgcccg
     3301 ccggcagcag ctgggctggg aggcctggct gcagtacagt ttccccctgc agctggagcc
     3361 ctcggctcaa acctgggggc ctggtaccct gcggctcccg aaccgggccc ttctggtcaa
     3421 cgttaagttt gagggcagcg aggagagctt caccttccag gtgtccacca aggacgtgcc
     3481 gctggcgctg atggcctgtg ccctgcggaa gaaggccaca gtgttccggc agccgctggt
     3541 ggagcagccg gaagactaca cgctgcaggt gaacggcagg catgagtacc tgtatggcag
     3601 ctacccgctc tgccagttcc agtacatctg cagctgcctg cacagtgggt tgacccctca
     3661 cctgaccatg gtccattcct cctccatcct cgccatgcgg gatgagcaga gcaaccctgc
     3721 cccccaggtc cagaaaccgc gtgccaaacc acctcccatt cctgcgaaga agccttcctc
     3781 tgtgtccctg tggtccctgg agcagccgtt ccgcatcgag ctcatccagg gcagcaaagt
     3841 gaacgccgac gagcggatga agctggtggt gcaggccggg cttttccacg gcaacgagat
     3901 gctgtgcaag acggtgtcca gctcggaggt gagcgtgtgc tcggagcccg tgtggaagca
     3961 gcggctggag ttcgacatca acatctgcga cctgccccgc atggcccgtc tctgctttgc
     4021 gctgtacgcc gtgatcgaga aagccaagaa ggctcgctcc accaagaaga agtccaagaa
     4081 ggcggactgc cccattgcct gggccaacct catgctgttt gactacaagg accagcttaa
     4141 gaccggggaa cgctgcctct acatgtggcc ctccgtccca gatgagaagg gcgagctgct
     4201 gaaccccacg ggcactgtgc gcagtaaccc caacacggat agcgccgctg ccctgctcat
     4261 ctgcctgccc gaggtggccc cgcaccccgt gtactacccc gccctggaga agatcttgga
     4321 gctggggcga cacagcgagt gtgtgcatgt caccgaggag gagcagctgc agctgcggga
     4381 aatcctggag cggcgggggt ctggggagct gtatgagcac gagaaggacc tggtgtggaa
     4441 gctgcggcat gaagtccagg agcacttccc ggaggcgcta gcccggctgc tgctggtcac
     4501 caagtggaac aagcatgagg atgtggccca gatgctctac ctgctgtgct cctggccgga
     4561 gctgcccgtc ctgagcgccc tggagctgct agacttcagc ttccccgatt gccacgtagg
     4621 ctccttcgcc atcaagtcgc tgcggaaact gacggacgat gagctgttcc agtacctgct
     4681 gcagctggtg caggtgctca agtacgagtc ctacctggac tgcgagctga ccaaattcct
     4741 gctggaccgg gccctggcca accgcaagat cggccacttc cttttctggc acctccgctc
     4801 cgagatgcac gtgccgtcgg tggccctgcg cttcggcctc atcctggagg cctactgcag
     4861 gggcagcacc caccacatga aggtgctgat gaagcagggg gaagcactga gcaaactgaa
     4921 ggccctgaat gacttcgtca agctgagctc tcagaagacc cccaagcccc agaccaagga
     4981 gctgatgcac ttgtgcatgc ggcaggaggc ctacctagag gccctctccc acctgcagtc
     5041 cccactcgac cccagcaccc tgctggctga agtctgcgtg gagcagtgca ccttcatgga
     5101 ctccaagatg aagcccctgt ggatcatgta cagcaacgag gaggcaggca gcggcggcag
     5161 cgtgggcatc atctttaaga acggggatga cctccggcag gacatgctga ccctgcagat
     5221 gatccagctc atggacgtcc tgtggaagca ggaggggctg gacctgagga tgacccccta
     5281 tggctgcctc cccaccgggg accgcacagg cctcattgag gtggtactcc gttcagacac
     5341 catcgccaac atccaactca acaagagcaa catggcagcc acagccgcct tcaacaagga
     5401 tgccctgctc aactggctga agtccaagaa cccgggggag gccctggatc gagccattga
     5461 ggagttcacc ctctcctgtg ctggctattg tgtggccaca tatgtgctgg gcattggcga
     5521 tcggcacagc gacaacatca tgatccgaga gagtgggcag ctgttccaca ttgattttgg
     5581 ccactttctg gggaatttca agaccaagtt tggaatcaac cgcgagcgtg tcccattcat
     5641 cctcacctac gactttgtcc atgtgattca gcaggggaag actaataata gtgagaaatt
     5701 tgaacggttc cggggctact gtgaaagggc ctacaccatc ctgcggcgcc acgggcttct
     5761 cttcctccac ctctttgccc tgatgcgggc ggcaggcctg cctgagctca gctgctccaa
     5821 agacatccag tatctcaagg actccctggc actggggaaa acagaggagg aggcactgaa
     5881 gcacttccga gtgaagttta acgaagccct ccgtgagagc tggaaaacca aagtgaactg
     5941 gctggcccac aacgtgtcca aagacaacag gcagtaggga tcccgcgact ctagataatt
     6001 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc
     6061 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg
     6121 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct
     6181 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt
     6241 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg
     6301 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg
     6361 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag
     6421 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc
     6481 ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttaccatgac cgagtacaag
     6541 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     6601 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     6661 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     6721 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     6781 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     6841 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     6901 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     6961 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     7021 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     7081 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaccgcg tctggaacaa
     7141 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     7201 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     7261 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     7321 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     7381 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     7441 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     7501 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     7561 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     7621 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     7681 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattaattc
     7741 tgcagtcgag acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat
     7801 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct
     7861 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     7921 gaaaagaggg gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg
     7981 tggatctacc acacacaagg ctacttccct gattagcaga actacacacc agggccaggg
     8041 gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gccagataag
     8101 gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg
     8161 atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat
     8221 cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac
     8281 aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg
     8341 cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt
     8401 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     8461 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     8521 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     8581 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc
     8641 ttgacattgc tagcgtttta ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     8701 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     8761 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     8821 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     8881 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     8941 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     9001 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     9061 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     9121 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     9181 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     9241 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     9301 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     9361 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     9421 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     9481 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     9541 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     9601 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     9661 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     9721 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     9781 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     9841 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     9901 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     9961 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
    10021 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    10081 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    10141 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    10201 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    10261 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    10321 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    10381 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    10441 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    10501 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    10561 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    10621 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    10681 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    10741 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    10801 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
    10861 cgggagatca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
    10921 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
    10981 aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa
    11041 cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc
    11101 tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca
    11161 aacttagtag tttttaaaga aattgtattt gttaaatatg tactacaaac ttagtagt
//
