LOCUS       V020043                10347 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020043
VERSION     V020043
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2807..3523
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="Aequorea coerulescens GFP"
                     /transl_table=1
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
                     /label="AcGFP1"
     CDS             3557..5140
                     /label="Psip1(NM_133948)"
                     /note="Psip1(NM_133948)"
                     /gene="Psip1"
     promoter        5182..5681
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             5702..6301
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6315..6903
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7110..7743
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(7872..7888)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7896..7912
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7920..7950)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7965..7986
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8274..8862)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9033..9893)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9894..9998)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    10046..10180
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctaccggtc gccaccatgg tgagcaaggg
     2821 cgccgagctg ttcaccggca tcgtgcccat cctgatcgag ctgaatggcg atgtgaatgg
     2881 ccacaagttc agcgtgagcg gcgagggcga gggcgatgcc acctacggca agctgaccct
     2941 gaagttcatc tgcaccaccg gcaagctgcc tgtgccctgg cccaccctgg tgaccaccct
     3001 gagctacggc gtgcagtgct tctcacgcta ccccgatcac atgaagcagc acgacttctt
     3061 caagagcgcc atgcctgagg gctacatcca ggagcgcacc atcttcttcg aggatgacgg
     3121 caactacaag tcgcgcgccg aggtgaagtt cgagggcgat accctggtga atcgcatcga
     3181 gctgaccggc accgatttca aggaggatgg caacatcctg ggcaataaga tggagtacaa
     3241 ctacaacgcc cacaatgtgt acatcatgac cgacaaggcc aagaatggca tcaaggtgaa
     3301 cttcaagatc cgccacaaca tcgaggatgg cagcgtgcag ctggccgacc actaccagca
     3361 gaataccccc atcggcgatg gccctgtgct gctgcccgat aaccactacc tgtccaccca
     3421 gagcgccctg tccaaggacc ccaacgagaa gcgcgatcac atgatctact tcggcttcgt
     3481 gaccgccgcc gccatcaccc acggcatgga tgagctgtac aagtccggac tcagatctcg
     3541 aggaggaggg ggaagcatga ctcgcgattt caaacctgga gacctcatct tcgccaagat
     3601 gaaaggttat cctcattggc cagctcgagt agatgaagtt cctgatggag ctgtaaaacc
     3661 acccacaaac aaactaccca ttttcttttt tggaacccat gagactgctt ttttaggacc
     3721 aaaggacata tttccttatt cagaaaataa ggaaaagtat ggcaaaccaa ataaacggaa
     3781 aggttttaat gaaggattgt gggagataga taacaatccg aaagtgaaat tttcaagtca
     3841 acaggcatca actaaacaat ccaatgcatc gtctgatgtt gaagtggaag aaaaagagac
     3901 taacgtttca aaggaagaca ctgatcagga agaaaaggcc agcaatgagg atgtgactaa
     3961 agcagttgac ataaccactc caaaagctgc caggcgagga agaaagagaa aggctgaaaa
     4021 acaagtagac actgaagagg cgggaatggt gactgcagca accgcttcta atgtgaaagc
     4081 aagtcctaag agaggacgac ctgcagctac tgaagtcaag attcccaaac caagaggcag
     4141 acctaaagtg gtaaagcagc cttgtccttc agacggtgac atggttattg atgaagataa
     4201 aagtaaaaaa aagggaccag aggagaaaca acctaaaaag cagcttaaaa aagaggaaga
     4261 aggccaaaag gaagaagaga agccaagaaa agaaccagat aagaaagaag ggaagaaaga
     4321 agttgaatct aaacggaaaa atttagctaa accaggggtt acatcaacct ctgattcaga
     4381 agacgaagac gatcaagagg gtgaaaagaa gagaaagggt ggaaggaact tccaggctgc
     4441 tcacaggagg aacatgctaa aaggccaaca tgagaaagaa gctggagatc ggaaacgcaa
     4501 gcaggaggaa caaatggaaa ctgagcagca gaataaagat gaaggaaaga agccagaagt
     4561 taagaaagtg gagaagaagc gagaaacatc aatggattct cgacttcaaa ggatacatgc
     4621 tgaaattaaa aattcactca aaattgataa tctagacgtg aacagatgca tagaggccct
     4681 ggatgaactc gcttcacttc aggtaacaat gcagcaagct cagaaacaca cagagatgat
     4741 cacaaccctg aagaaaatac ggcggttcaa agtcagtcaa gttatcatgg aaaagtctac
     4801 aatgttatat aacaagttta agaatatgtt tttggttggt gaaggagatt cagtcatcac
     4861 ccaagtgctg aacaaatctc ttgctgaaca gagacagcat gaggaagcaa ataaaaccaa
     4921 agatcagggg aagaaagggc caaacaaaaa gctagagaag gaaccaacag gtacgaagag
     4981 cctaaatggt ggatctgatg ctcaagagag taatcatcca cagcataatg gtgatagtaa
     5041 cgaagacggc aaagacagcc gcgaggccag cagtaagaca aagccacctg gagaagagag
     5101 agaggctgaa atatctctga aagagtctac actagataac tagggatcca ccggatctag
     5161 ataactgatc ataattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat
     5221 gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac
     5281 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
     5341 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
     5401 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
     5461 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
     5521 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg
     5581 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt
     5641 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac
     5701 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     5761 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     5821 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     5881 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     5941 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     6001 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     6061 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     6121 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     6181 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     6241 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     6301 accgcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     6361 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     6421 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     6481 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     6541 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     6601 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     6661 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     6721 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     6781 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     6841 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     6901 cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa
     6961 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg
     7021 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct
     7081 tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca
     7141 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta
     7201 cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc
     7261 agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc
     7321 tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag
     7381 ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg
     7441 atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg
     7501 cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta
     7561 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     7621 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     7681 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     7741 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     7801 cagagagtga gaggccttga cattgctagc gttttaccgt cgacctctag ctagagcttg
     7861 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     7921 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     7981 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     8041 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     8101 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     8161 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     8221 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     8281 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     8341 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     8401 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     8461 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     8521 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     8581 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     8641 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     8701 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     8761 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     8821 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     8881 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     8941 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     9001 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     9061 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     9121 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     9181 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     9241 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     9301 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     9361 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     9421 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     9481 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     9541 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     9601 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     9661 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     9721 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     9781 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     9841 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     9901 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     9961 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    10021 cctgacgtcg acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata
    10081 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    10141 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
    10201 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt
    10261 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg
    10321 ttaaatatgt actacaaact tagtagt
//
