LOCUS       V021461                13592 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021461
VERSION     V021461
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2826..8327
                     /label="SLX4(NM_032444)"
                     /note="SLX4(NM_032444)"
                     /gene="SLX4"
     CDS             8334..8360
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        8380..8879
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             8900..9499
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    9513..10101
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             10308..10941
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(11070..11086)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    11094..11110
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(11118..11148)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    11163..11184
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(11472..12060)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(12231..13091)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(13092..13196)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    13244..13378
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ccaccatgaa actgagtgtg aatgaggctc agctaggctt ctacttgggt tcactttctc
     2881 atctgtctgc ctgtcctggg attgaccctc gctcctctga agaccagcct gaaagcctta
     2941 aaactggtca gatgatggat gagtctgatg aggactttaa agaactctgc gctagctttt
     3001 tccaaagggt gaaaaaacat ggaatcaagg aagtgtcagg agaaaggaag acacaaaagg
     3061 ctgcctcaaa cggcactcag ataagaagca aattgaaaag gaccaaacaa actgctacca
     3121 agaccaaaac ccttcaaggc cctgcagaga agaaacctcc gtctggcagc caggccccta
     3181 ggactaaaaa gcaaagggta accaaatggc aagcaagtga accggcccac tctgtgaatg
     3241 gggagggggg tgtgcttgcc tctgctccag atccacctgt gctccgggaa acagcacaaa
     3301 acacccagac gggtaaccag caagaaccat cgccaaacct ttccagagag aaaaccagag
     3361 agaatgtgcc caacagcgac tcccagcctc ctccttcctg tttgacaaca gcagtgccaa
     3421 gtccctccaa accccgcaca gcacaattgg tcctacagcg aatgcagcag ttcaagagag
     3481 cagaccccga gcgtttgaga cacgcttcag aagagtgctc cctcgaggct gcgcgggaag
     3541 aaaatgtccc aaaggatcct caagaggaga tgatggcggg gaatgtgtat gggcttgggc
     3601 cccctgcccc agagagcgac gctgcggtgg ccttgaccct gcagcaggag tttgcacggg
     3661 taggagcatc ggcacatgat gatagcctgg aggaaaaggg tttgttcttc tgccagattt
     3721 gtcaaaagaa cctctcagcc atgaacgtga cccgaaggga acagcatgtg aacaggtgct
     3781 tggatgaagc tgaaaagaca ctaagacctt ctgtgcctca gatccctgag tgcccgattt
     3841 gtgggaaacc gtttcttacc ttaaagagca gaaccagtca cttgaagcag tgtgctgtga
     3901 agatggaggt tggcccccag ctcctgcttc aggctgtgcg gctgcagaca gcacagcctg
     3961 agggtagcag cagcccaccc atgttcagct tcagtgatca cagtagaggt ctgaaacgga
     4021 gaggacccac cagcaagaag gagccacgga agaggcggaa ggtggacgag gcaccgtccg
     4081 aggacctgct ggtggccatg gctctgtccc ggtcggagat ggagccgggt gcggctgtac
     4141 cagcgctcag gctggaaagt gccttttctg agaggataag accagaagca gagaataaaa
     4201 gtcgcaagaa gaaacccccg gtatcccccc cattgttgtt agtccaggac tctgaaacca
     4261 caggccgaca gatagaggac cgtgtggccc tgctcctctc tgaggaagtg gaattgtcta
     4321 gcacgccacc acttcctgcc agcaggattt taaaggaagg gtgggaaaga gcgggccagt
     4381 gtcctcctcc acctgaacgc aagcagagct ttctgtggga gggcagcgca ctgactgggg
     4441 cctgggccat ggaggacttc tacacggcca ggctggtccc tcctctcgtg ccccagcggc
     4501 ctgcccaggg ccttatgcag gagcccgtgc cgcctctggt gccacctgag cactcagagc
     4561 tgagcgagcg aaggtcaccc gctctccacg gcacccccac tgcaggctgt ggctccaggg
     4621 gcccgtcgcc ttcggccagc cagagggagc accaggccct gcaggacctc gtggacctgg
     4681 cgagggaggg actgagcgcc agcccgtggc ccggcagtgg gggcctggct ggctcggaag
     4741 ggactgcagg gttggacgtg gtgcccggcg gccttcctct gactgggttt gtggtgccat
     4801 cgcaggacaa gcacccggac aggggcggcc gcaccttgct ctccctcggg ctgctggttg
     4861 ctgactttgg cgccatggtc aataacccac acctgagtga tgtccagttt cagacggaca
     4921 gcggggaggt gctttacgcc cacaagttcg tgctttatgc ccgatgcccg ctcctcatcc
     4981 agtatgtgaa caatgaaggc ttctccgctg tagaggacgg ggttctgacc cagcgtgtcc
     5041 tgctgggtga cgtgagcacc gaggccgccc gcacgttcct gcactatctc tacactgcgg
     5101 acactggcct tcctcctggc cttagctctg agctgagctc cctggcccac aggtttggcg
     5161 tgagtgagct cgttcacctg tgcgaacagg tgcctattgc cactgactca gagggcaaac
     5221 catgggagga gaaggaagca gagaattgcg aaagcagggc cgagaatttc caggaactct
     5281 tgaggtcaat gtgggcagat gaagaggagg aagcggagac tttgttgaaa tccaaggacc
     5341 acgaagaaga tcaagaaaac gtgaatgaag cagaaatgga agaaatttat gaatttgcag
     5401 ctactcagcg aaagcttctc caggaagaaa gggcagcggg tgccggcgag gacgctgact
     5461 ggctggaggg tggcagtccg gtttctgggc aactcctagc aggtgtccag gtgcagaaac
     5521 agtgggacaa ggtggaggag atggagccgt tggagccagg aagagatgag gccgccacca
     5581 cctgggagaa gatgggacag tgcgctctcc cgccacccca gggccagcac tcaggggcac
     5641 ggggagcaga ggcccctgag caggaggcgc cagaggaggc gcttggccat tccagctgct
     5701 ccagcccttc cagggactgc caggcagaga gaaaagaagg ctctcttccg cactcagatg
     5761 atgccgggga ttacgaacag ctcttctcat caactcaggg agagatctca gagccgtccc
     5821 aaataacaag tgagcccgag gaacaaagtg gcgctgtcag ggaaaggggg ctggaggttt
     5881 ctcatcgcct ggctccctgg caggcatctc caccgcaccc gtgccgcttc ctattggggc
     5941 ctccccaggg cgggagtccc cgcgggtctc atcacacaag tgggtcgtcc ctgtcaacac
     6001 cccggtcccg tggcggaact tcccaggtgg gctccccaac cttgctgtct ccagctgtgc
     6061 catcaaagca gaaaagggac aggagcatcc tcacgctgtc taaagagcca gggcaccaga
     6121 aaggcaaaga gcgtcggtcc gtgctggagt gcagaaataa gggggtcctg atgttcccag
     6181 aaaaatctcc gtctattgac ctaacccagt caaatcctga ccattcgagc tccagatctc
     6241 agaaatcttc atccaaactg aacgaagaag atgaggtcat cctcttactg gactcggatg
     6301 aggagctgga gctagaacaa accaaaatga agtccatttc tagtgatcct ctggaagaaa
     6361 agaaagctct agaaattagc cctaggtcct gtgagctgtt ttccatcatt gatgttgatg
     6421 cagatcagga accttcccag agcccaccaa gaagcgaagc tgtgctgcag caggaggatg
     6481 agggggcgct gccggagaat cggggctctt tgggcaggag aggggctccc tggctgttct
     6541 gtgaccgtga gagcagcccc agcgaggcca gcaccacaga cacctcgtgg ctggtgcccg
     6601 ccaccccgct ggccagcaga agccgtgact gttcttccca gacccaaatc agcagcctca
     6661 ggagcgggct ggccgtgcag gcggtgactc agcacacgcc cagggcctca gtaggaaaca
     6721 gggaagggaa cgaagtcgca cagaagtttt ctgtcatcag gccccagaca ccaccgcccc
     6781 agacaccgtc ctcatgcctc actcccgtct ctccaggaac ttctgacggc agaaggcaag
     6841 gccacagaag cccttcccgt ccccaccccg ggggccaccc gcactcctct ccgctggctc
     6901 cacatcccat ctcaggggac cgcgcccact tcagcaggcg gttcctgaaa cactcgccgc
     6961 ctgggccaag cttcctgaac cagaccccag cgggtgaagt ggtggaagtc ggagacagtg
     7021 acgatgagca ggaggtggcc tcccatcagg ccaacagaag ccccccactg gacagtgacc
     7081 ccccaattcc aattgacgac tgctgctggc acatggagcc cctctcgcca attcccattg
     7141 accactggaa cctggagcgg accggccccc tgagcaccag cagccccagc cgcaggatga
     7201 acgaggccgc cgacagccgt gactgtcgct ccccgggact cctggacacc acccccatcc
     7261 gaggaagctg cactacccag aggaaattgc aagagaagtc ctcgggcgcg ggctccctgg
     7321 ggaacagcag gccgagcttt ctgaattcgg ctctgtggga cgtttgggac ggggaagagc
     7381 agaggcctcc agagacccct cctccggccc agatgccaag cgctggtgga gctcagaagc
     7441 ccgaagggtt agagacaccc aaaggtgcta atcggaagaa gaacttgccc cccaaagtgc
     7501 ccataacgcc gatgccacag tattccatta tggagacgcc ggtgctgaag aaggaactgg
     7561 ataggtttgg agtccgccct ctgcctaaac gccagatggt tctgaagctg aaggagatat
     7621 tccagtacac tcaccagacc ctggactcag actccgagga cgagagccag tcctcacagc
     7681 cgctgttgca ggcgcctcac tgccagaccc tcgcctccca gacctacaag ccttcaaggg
     7741 caggggtcca tgcccagcag gaggccacca caggacctgg ggcccatagg cccaagggac
     7801 ctgctaagac caagggcccc cgacatcaaa ggaagcatca tgaaagcatc acacccccaa
     7861 gcaggtcgcc caccaaggag gcacctccag gcctcaatga tgacgcccag atcccagcct
     7921 ctcaagaatc cgtggccacc tctgtggatg gcagtgacag ctccttgagc tcacagagtt
     7981 cttcctcctg tgagtttgga gcggcatttg agtctgcagg tgaagaggag ggcgaggggg
     8041 aggtcagtgc ctcgcaggca gccgtgcagg cggcggacac agacgaggcg ctgaggtgct
     8101 acatccgctc caagccggcc ctgtaccaga aggtgctgct gtaccagccc tttgagctgc
     8161 gggagctgca ggcagagctg aggcagaacg gcctccgtgt gtcctcgcgc aggctgttgg
     8221 acttcctgga cacccactgt atcaccttca ccactgccgc cacccgcagg gagaagctcc
     8281 agggcaggag gcggcagcct cggggcaaga agaaggtgga gcggaacgga tcctacccat
     8341 acgacgtacc agattacgct tagtctagat aattctaccg ggtaggggag gcgcttttcc
     8401 caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg
     8461 gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg
     8521 gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc
     8581 agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga
     8641 tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc
     8701 tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca
     8761 ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca
     8821 cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga
     8881 cctgcagccc aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     8941 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     9001 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     9061 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     9121 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     9181 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     9241 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     9301 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     9361 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     9421 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     9481 ccgcaagccc ggtgcctgac cgcgtctgga acaatcaacc tctggattac aaaatttgtg
     9541 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     9601 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     9661 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     9721 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     9781 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     9841 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     9901 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     9961 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
    10021 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
    10081 ccctttgggc cgcctccccg cctggaatta attctgcagt cgagacctag aaaaacatgg
    10141 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca
    10201 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta
    10261 caaggcagct gtagatctta gccacttttt aaaagaaaag aggggactgg aagggctaat
    10321 tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt
    10381 ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg
    10441 gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa
    10501 caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt
    10561 agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga
    10621 gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc
    10681 agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc
    10741 agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
    10801 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
    10861 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
    10921 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
    10981 ttgcaaagaa atgaatatca gagagtgaga ggccttgaca ttgctagcgt tttaccgtcg
    11041 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
    11101 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
    11161 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
    11221 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
    11281 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
    11341 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
    11401 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
    11461 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
    11521 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
    11581 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
    11641 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
    11701 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
    11761 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
    11821 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
    11881 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
    11941 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
    12001 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
    12061 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
    12121 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
    12181 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
    12241 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
    12301 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
    12361 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
    12421 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    12481 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    12541 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    12601 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    12661 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    12721 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    12781 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    12841 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    12901 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    12961 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    13021 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    13081 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    13141 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    13201 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcaacttgt ttattgcagc
    13261 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
    13321 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
    13381 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
    13441 tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt
    13501 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttttta aagaaattgt
    13561 atttgttaaa tatgtactac aaacttagta gt
//
