LOCUS V035410 9034 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V035410 VERSION V035410 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2843..3730 /label="SULT1B1(NM_014465)" /note="SULT1B1(NM_014465)" /gene="SULT1B1" CDS 3737..3802 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 3822..4321 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 4342..4941 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 4955..5543 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 5750..6383 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(6512..6528) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6536..6552 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6560..6590) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6605..6626 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6914..7502) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7673..8533) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8534..8638) /gene="bla" /label="AmpR promoter" polyA_signal 8686..8820 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcgcca ccatgctttc cccaaaagat attctgcgaa aagatctgaa 2881 gttggtccat ggttatccca tgacctgtgc ttttgcaagc aactgggaaa aaattgaaca 2941 gttccatagc agaccagatg acattgtgat agccacttat cctaaatcag gtactacttg 3001 ggttagtgaa attatagaca tgattctaaa tgatggagat attgaaaaat gtaagcgagg 3061 ttttattact gaaaaagttc caatgttgga aatgactctc cctggattaa gaacatcagg 3121 tatagaacaa ttggagaaga atccatcacc ccggattgtg aaaacacatc taccgactga 3181 tcttcttcct aaatctttct gggaaaacaa ttgcaagatg atttatctgg ctcgtaatgc 3241 caaggatgtt tcagtctcat attaccattt tgacttaatg aataatttac agccttttcc 3301 tggtacctgg gaagaatatc tggagaaatt cttaactgga aaagtggcct atggttcctg 3361 gtttactcat gttaaaaact ggtggaagaa aaaggaagaa cacccaatac tttttttgta 3421 ctatgaagat atgaaagaga atccaaagga ggaaatcaag aagatcatta gatttctaga 3481 gaagaacctg aatgatgaga tcttggatag gatcatccat cacacctcat ttgaagtgat 3541 gaaggacaat cctttggtaa attatacaca tctaccaact acagtgatgg atcatagcaa 3601 atcccctttt atgcgtaaag ggacggctgg tgactggaag aattacttca ccgtggccca 3661 aaatgagaaa tttgatgcta tttatgagac agaaatgtcc aaaactgcac ttcaattccg 3721 cacagagatt ggatccgact acaaagacca tgacggtgat tataaagatc atgacatcga 3781 ctacaaggat gacgatgaca agtagtctag ataattctac cgggtagggg aggcgctttt 3841 cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag 3901 tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt 3961 tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc 4021 gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca 4081 gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca 4141 gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct 4201 caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg 4261 cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc 4321 gacctgcagc ccaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc 4381 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg 4441 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc 4501 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg 4561 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg 4621 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg 4681 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac 4741 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc 4801 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg 4861 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg 4921 acccgcaagc ccggtgcctg accgcgtctg gaacaatcaa cctctggatt acaaaatttg 4981 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc 5041 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta 5101 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt 5161 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca 5221 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc 5281 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt 5341 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg 5401 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg 5461 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat 5521 ctccctttgg gccgcctccc cgcctggaat taattctgca gtcgagacct agaaaaacat 5581 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca 5641 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact 5701 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta 5761 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac 5821 ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga 5881 tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag 5941 aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg 6001 ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg 6061 gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt 6121 ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa 6181 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct 6241 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca 6301 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta 6361 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata 6421 acttgcaaag aaatgaatat cagagagtga gaggccttga cattgctagc gttttaccgt 6481 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt 6541 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg 6601 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg 6661 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 6721 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 6781 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 6841 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 6901 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 6961 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 7021 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 7081 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 7141 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 7201 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 7261 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 7321 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc 7381 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 7441 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 7501 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 7561 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 7621 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 7681 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 7741 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 7801 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 7861 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 7921 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 7981 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 8041 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 8101 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 8161 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 8221 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 8281 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 8341 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 8401 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 8461 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 8521 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 8581 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 8641 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcaactt gtttattgca 8701 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 8761 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc 8821 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc 8881 actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga attattttca 8941 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagtttt taaagaaatt 9001 gtatttgtta aatatgtact acaaacttag tagt //