LOCUS       V026044                11056 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026044
VERSION     V026044
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2843..5752
                     /label="Slc8a1(NM_011406)"
                     /note="Slc8a1(NM_011406)"
                     /gene="Slc8a1"
     CDS             5759..5824
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5844..6343
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             6364..6963
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6977..7565
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7772..8405
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8534..8550)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8558..8574
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8582..8612)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8627..8648
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8936..9524)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9695..10555)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10556..10660)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    10708..10842
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattcgcca ccatgcttcg attaagtctc ccacccaatg tttcaatggg
     2881 atttcgtctg gtagctctgg tggctctctt gttttcccat gttgaccata taactgcaga
     2941 tacagaggca gaaacaggag gaaatgaaac cactgaatgt actggctcat attactgtaa
     3001 gaaaggggtg atcttgccca tttgggaacc ccaagaccca tcttttgggg acaaaattgc
     3061 tagagcaact gtgtattttg tggccatggt ctacatgttc cttggagttt ctattattgc
     3121 agaccggttt atgtcctcta tagaggtcat cacctctcaa gagaaagaaa taacgataaa
     3181 gaaaccgaat ggagagacca ccaagacgac ggtgagaatc tggaacgaga ctgtgtcgaa
     3241 cctgaccttg atggccctgg gatcttctgc tcctgagatt ctcctgtcag tcattgaagt
     3301 gtgcggccat aacttcaccg caggggacct gggtcccagc accatcgtgg gaagtgctgc
     3361 ctttaacatg ttcatcataa tcgcactctg tgtttacgtg gtccctgatg gagagacaag
     3421 gaagatcaag catctgcgtg tgttctttgt gacagcagcc tggagcatct ttgcctatac
     3481 ctggctttat ataatcttgt ctgtcagctc tcctggagtt gtggaggtct gggaaggctt
     3541 gcttactttc ttcttctttc ccatctgcgt tgtgttcgcg tgggtagcag acaggcggct
     3601 tctcttttac aagtatgtct acaagcggta cagggccggc aagcagaggg ggatgatcat
     3661 tgaacatgaa ggagacagac cagcttccaa aactgaaatc gaaatggatg ggaaagtggt
     3721 caactctcat gttgacaatt tcttagatgg ggctctggtt ttggaagttg atgagaggga
     3781 ccaagatgat gaggaagcca ggcgtgagat ggcaaggatt ctgaaggaac ttaagcagaa
     3841 gcatcctgag aaagaaattg agcaattaat agaattagcc aactaccagg tcctaagtca
     3901 acagcagaaa agccgagcat tttacaggat tcaagctact cgcctgatga ccggagctgg
     3961 caacatcttg aagaggcacg cagctgatca agcaaggaag gctgtcagta tgcatgaagt
     4021 caacatggaa atggctgaaa acgacccagt cagtaagatc ttctttgagc aaggaacata
     4081 ccagtgtcta gagaactgtg gtactgtggc cctcaccatt atgcgcagag ggggcgactt
     4141 gagcaccact gtgtttgttg acttcaggac agaagacggc acagccaatg ctgggtctga
     4201 ttatgaattc acggaaggga ctgtgatctt caaaccaggg gagacccaga aggaaatcag
     4261 agttggcatc attgatgatg atatctttga agaagatgaa aacttccttg tgcatcttag
     4321 caatgtcaga gtctcttcag atgtttcaga agatggcata ctagaatcca atcacgcttc
     4381 ttcaattgct tgtcttgggt cacccagcac tgccaccata accatttttg atgatgacca
     4441 tgcaggcatc tttacatttg aggaacccgt gactcacgtg agcgagagca ttggcatcat
     4501 ggaggtgaag gttttgagaa cctctggagc tcgaggaaat gttatcattc cctacaaaac
     4561 tattgaaggc acagcccgag gtggagggga agactttgag gacacctgtg gagagctcga
     4621 attccagaac gatgaaatag tcaaaacaat atcagtcaag gtaatcgatg acgaggagta
     4681 tgagaaaaac aagaccttct tcattgagat tggagagccc cgtctggtgg agatgagtga
     4741 gaagaaagcc ctgttgttga atgagcttgg tggcttcaca ttaacaggaa aagagatgta
     4801 tggccaacct atcttcagga aggtccatgc tagagatcat ccgattccct ctactgtaat
     4861 caccatctca gaggaatatg atgacaagca gccactgacc agcaaagaag aggaggagag
     4921 gcgcattgcg gaaatggggc gccccatcct aggcgagcac accaagctgg aggtgatcat
     4981 cgaagagtct tacgaattca agagcactgt ggacaaactc attaagaaga cgaacctggc
     5041 ccttgtggtg gggaccaaca gctggagaga gcagtttatc gaagccatca ctgtcagcgc
     5101 tggggaagat gacgatgatg atgaatgtgg ggaggagaag ctgccctcct gttttgatta
     5161 cgtgatgcac tttctcacag tgttctggaa ggttctgttt gccttcgtcc cacctacaga
     5221 atactggaat ggctgggcct gcttcattgt ctccatcctc atgatcggcc tactgaccgc
     5281 cttcattgga gacctggctt cccactttgg ctgcaccatt ggtctgaaag attccgtgac
     5341 tgccgttgtg tttgttgctc ttggaacctc ggtgccagac acatttgcca gcaaagtagc
     5401 agctacccag gaccagtatg cagatgcgtc tataggcaat gtcactggaa gcaatgctgt
     5461 gaatgtcttc ctgggaatcg gcgtggcctg gtccattgct gccatctacc atgcggccaa
     5521 cggggaacag ttcaaagtgt cccccggcac gctagctttc tctgtcactc tcttcactat
     5581 ttttgctttc atcaacgtgg gggtgctgct gtatcggcgg aggccagaaa taggaggtga
     5641 gctgggaggg ccccggactg ccaagctcct cacatcttcc ctgtttgtgc tcctgtggct
     5701 cttgtacatt ttcttctcct ccctggaagc ctactgccac ataaagggct tcggatccga
     5761 ctacaaagac catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga
     5821 caagtagtct agataattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc
     5881 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac
     5941 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc
     6001 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
     6061 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
     6121 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
     6181 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg
     6241 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac
     6301 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt
     6361 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     6421 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     6481 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     6541 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     6601 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     6661 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     6721 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     6781 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     6841 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     6901 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     6961 tgaccgcgtc tggaacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     7021 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     7081 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     7141 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     7201 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     7261 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     7321 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     7381 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     7441 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     7501 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     7561 cccgcctgga attaattctg cagtcgagac ctagaaaaac atggagcaat cacaagtagc
     7621 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg
     7681 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat
     7741 cttagccact ttttaaaaga aaagagggga ctggaagggc taattcactc ccaacgaaga
     7801 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac
     7861 tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta
     7921 ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac
     7981 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac
     8041 agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc
     8101 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
     8161 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
     8221 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     8281 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     8341 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     8401 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     8461 atcagagagt gagaggcctt gacattgcta gcgttttacc gtcgacctct agctagagct
     8521 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     8581 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     8641 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     8701 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     8761 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     8821 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     8881 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     8941 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     9001 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     9061 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     9121 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     9181 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     9241 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     9301 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     9361 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     9421 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     9481 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     9541 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     9601 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     9661 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     9721 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     9781 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     9841 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     9901 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     9961 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
    10021 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
    10081 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
    10141 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    10201 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    10261 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    10321 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    10381 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    10441 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    10501 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    10561 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    10621 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    10681 cacctgacgt cgacggatcg ggagatcaac ttgtttattg cagcttataa tggttacaaa
    10741 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
    10801 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct
    10861 aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg
    10921 tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt
    10981 tgttaaatat gtactacaaa cttagtagtt tttaaagaaa ttgtatttgt taaatatgta
    11041 ctacaaactt agtagt
//
