LOCUS       V023783                10811 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V023783
VERSION     V023783
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2826..3542
                     /codon_start=1
                     /note=" mammalian codon-optimized"
                     /product="enhanced monomeric variant of CFP (Rizzo et al.,
                     2004)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNG
                     IKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="mCerulean"
     CDS             3579..5543
                     /label="TNFRSF21(NM_014452)"
                     /note="TNFRSF21(NM_014452)"
                     /gene="TNFRSF21"
     CDS             5550..5579
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     promoter        5599..6098
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             6119..6718
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6732..7320
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7527..8160
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8289..8305)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8313..8329
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8337..8367)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8382..8403
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8691..9279)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9450..10310)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10311..10415)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    10463..10597
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     2881 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     2941 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     3001 ccaccctcgt gaccaccctg acctggggcg tgcagtgctt cgcccgctac cccgaccaca
     3061 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     3121 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     3181 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     3241 ggcacaagct ggagtacaac gccatcagcg acaacgtcta tatcaccgcc gacaagcaga
     3301 agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     3361 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     3421 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     3481 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     3541 agggtggcgg aggttcaggc ggtggaggat ctgaattcat ggggacctct ccgagcagca
     3601 gcaccgccct cgcctcctgc agccgcatcg cccgccgagc cacagccacg atgatcgcgg
     3661 gctcccttct cctgcttgga ttccttagca ccaccacagc tcagccagaa cagaaggcct
     3721 cgaatctcat tggcacatac cgccatgttg accgtgccac cggccaggtg ctaacctgtg
     3781 acaagtgtcc agcaggaacc tatgtctctg agcattgtac caacacaagc ctgcgcgtct
     3841 gcagcagttg ccctgtgggg acctttacca ggcatgagaa tggcatagag aaatgccatg
     3901 actgtagtca gccatgccca tggccaatga ttgagaaatt accttgtgct gccttgactg
     3961 accgagaatg cacttgccca cctggcatgt tccagtctaa cgctacctgt gccccccata
     4021 cggtgtgtcc tgtgggttgg ggtgtgcgga agaaagggac agagactgag gatgtgcggt
     4081 gtaagcagtg tgctcggggt accttctcag atgtgccttc tagtgtgatg aaatgcaaag
     4141 catacacaga ctgtctgagt cagaacctgg tggtgatcaa gccggggacc aaggagacag
     4201 acaacgtctg tggcacactc ccgtccttct ccagctccac ctcaccttcc cctggcacag
     4261 ccatctttcc acgccctgag cacatggaaa cccatgaagt cccttcctcc acttatgttc
     4321 ccaaaggcat gaactcaaca gaatccaact cttctgcctc tgttagacca aaggtactga
     4381 gtagcatcca ggaagggaca gtccctgaca acacaagctc agcaaggggg aaggaagacg
     4441 tgaacaagac cctcccaaac cttcaggtag tcaaccacca gcaaggcccc caccacagac
     4501 acatcctgaa gctgctgccg tccatggagg ccactggggg cgagaagtcc agcacgccca
     4561 tcaagggccc caagagggga catcctagac agaacctaca caagcatttt gacatcaatg
     4621 agcatttgcc ctggatgatt gtgcttttcc tgctgctggt gcttgtggtg attgtggtgt
     4681 gcagtatccg gaaaagctcg aggactctga aaaaggggcc ccggcaggat cccagtgcca
     4741 ttgtggaaaa ggcagggctg aagaaatcca tgactccaac ccagaaccgg gagaaatgga
     4801 tctactactg caatggccat ggtatcgata tcctgaagct tgtagcagcc caagtgggaa
     4861 gccagtggaa agatatctat cagtttcttt gcaatgccag tgagagggag gttgctgctt
     4921 tctccaatgg gtacacagcc gaccacgagc gggcctacgc agctctgcag cactggacca
     4981 tccggggccc cgaggccagc ctcgcccagc taattagcgc cctgcgccag caccggagaa
     5041 acgatgttgt ggagaagatt cgtgggctga tggaagacac cacccagctg gaaactgaca
     5101 aactagctct cccgatgagc cccagcccgc ttagcccgag ccccatcccc agccccaacg
     5161 cgaaacttga gaattccgct ctcctgacgg tggagccttc cccacaggac aagaacaagg
     5221 gcttcttcgt ggatgagtcg gagccccttc tccgctgtga ctctacatcc agcggctcct
     5281 ccgcgctgag caggaacggt tcctttatta ccaaagaaaa gaaggacaca gtgttgcggc
     5341 aggtacgcct ggacccctgt gacttgcagc ctatctttga tgacatgctc cactttctaa
     5401 atcctgagga gctgcgggtg attgaagaga ttccccaggc tgaggacaaa ctagaccggc
     5461 tattcgaaat tattggagtc aagagccagg aagccagcca gaccctcctg gactctgttt
     5521 atagccatct tcctgacctg ctgggatccg agcagaagct gatctcagag gaggacctgt
     5581 agtctagata attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg
     5641 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
     5701 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
     5761 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
     5821 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
     5881 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg
     5941 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg
     6001 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
     6061 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat
     6121 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     6181 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     6241 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     6301 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     6361 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     6421 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     6481 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     6541 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     6601 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     6661 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacc
     6721 gcgtctggaa caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     6781 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     6841 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     6901 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     6961 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     7021 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     7081 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     7141 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     7201 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     7261 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     7321 ctggaattaa ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
     7381 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
     7441 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     7501 ccacttttta aaagaaaaga ggggactgga agggctaatt cactcccaac gaagacaaga
     7561 tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc agaactacac
     7621 accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     7681 tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt
     7741 gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg
     7801 cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata
     7861 tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg
     7921 gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg
     7981 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     8041 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     8101 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     8161 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     8221 agagtgagag gccttgacat tgctagcgtt ttaccgtcga cctctagcta gagcttggcg
     8281 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     8341 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     8401 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     8461 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     8521 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     8581 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     8641 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     8701 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     8761 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     8821 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     8881 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     8941 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     9001 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     9061 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     9121 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     9181 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     9241 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     9301 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     9361 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     9421 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     9481 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     9541 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     9601 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     9661 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     9721 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     9781 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     9841 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     9901 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     9961 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    10021 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    10081 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    10141 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    10201 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    10261 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    10321 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    10381 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    10441 gacgtcgacg gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag
    10501 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    10561 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
    10621 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
    10681 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
    10741 aatatgtact acaaacttag tagtttttaa agaaattgta tttgttaaat atgtactaca
    10801 aacttagtag t
//
