LOCUS       V032005                 9049 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032005
VERSION     V032005
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2843..3784
                     /label="TREX1(NM_033629)"
                     /note="TREX1(NM_033629)"
                     /gene="TREX1"
     CDS             3791..3817
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        3837..4336
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             4357..4956
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4970..5558
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5765..6398
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(6527..6543)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6551..6567
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6575..6605)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6620..6641
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6929..7517)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7688..8548)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8549..8653)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    8701..8835
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattcgcca ccatgggctc gcaggccctg cccccggggc ccatgcagac
     2881 cctcatcttt ttcgacatgg aggccactgg cttgcccttc tcccagccca aggtcacgga
     2941 gctgtgcctg ctggctgtcc acagatgtgc cctggagagc ccccccacct ctcaggggcc
     3001 acctcccaca gttcctccac caccgcgtgt ggtagacaag ctctccctgt gtgtggctcc
     3061 ggggaaggcc tgcagccctg cagccagcga gatcacaggt ctgagcacag ctgtgctggc
     3121 agcgcatggg cgtcaatgtt ttgatgacaa cctggccaac ctgctcctag ccttcctgcg
     3181 gcgccagcca cagccctggt gcctggtggc acacaatggt gaccgctacg acttccccct
     3241 gctccaagca gagctggcta tgctgggcct caccagtgct ctggatggtg ccttctgtgt
     3301 ggatagcatc actgcgctga aggccctgga gcgagcaagc agcccctcag aacacggccc
     3361 aaggaagagc tatagcctag gcagcatcta cactcgcctg tatgggcagt cccctccaga
     3421 ctcgcacacg gctgagggtg atgtcctggc cctgctcagc atctgtcagt ggagaccaca
     3481 ggccctgctg cggtgggtgg atgctcacgc caggcctttc ggcaccatca ggcccatgta
     3541 tggggtcaca gcctctgcta ggaccaagcc aagaccatct gctgtcacaa ccactgcaca
     3601 cctggccaca accaggaaca ctagtcccag ccttggagag agcaggggta ccaaggatct
     3661 tcctccagtg aaggaccctg gagccctatc cagggagggg ctgctggccc cactgggtct
     3721 gctggccatc ctgaccttgg cagtagccac actgtatgga ctatccctgg ccacacctgg
     3781 ggagggatcc tacccatacg acgtaccaga ttacgcttag tctagataat tctaccgggt
     3841 aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac
     3901 ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca
     3961 accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa
     4021 gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct
     4081 cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg
     4141 cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg
     4201 gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg
     4261 aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc
     4321 atctccgggc ctttcgacct gcagcccaag cttaccatga ccgagtacaa gcccacggtg
     4381 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     4441 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     4501 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     4561 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     4621 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     4681 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     4741 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     4801 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     4861 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     4921 cgcacctggt gcatgacccg caagcccggt gcctgaccgc gtctggaaca atcaacctct
     4981 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     5041 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     5101 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     5161 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     5221 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     5281 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     5341 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     5401 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     5461 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     5521 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga
     5581 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
     5641 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
     5701 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg
     5761 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac
     5821 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
     5881 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
     5941 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
     6001 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
     6061 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
     6121 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
     6181 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
     6241 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     6301 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     6361 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     6421 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg
     6481 ctagcgtttt accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     6541 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     6601 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     6661 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     6721 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     6781 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     6841 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     6901 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     6961 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7021 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7081 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     7141 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7201 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7261 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7321 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     7381 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7441 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     7501 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7561 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7621 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7681 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7741 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7801 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     7861 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     7921 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     7981 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     8041 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     8101 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     8161 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8221 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8281 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     8341 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     8401 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     8461 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     8521 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     8581 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     8641 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     8701 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     8761 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     8821 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
     8881 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc
     8941 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
     9001 gtttttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
