LOCUS       V024142                 9621 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V024142
VERSION     V024142
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2826..4256
                     /label="TRIM72(NM_001008274)"
                     /note="TRIM72(NM_001008274)"
                     /gene="TRIM72"
     CDS             4263..4352
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     promoter        4409..4908
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             4929..5528
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5542..6130
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6337..6970
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(7099..7115)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7123..7139
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7147..7177)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7192..7213
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7501..8089)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8260..9120)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9121..9225)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    9273..9407
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ccaccatgtc ggctgcgccc ggcctcctgc accaggagct gtcctgcccg ctgtgcctgc
     2881 agctgttcga cgcgcccgtg acagccgagt gcggccacag tttctgccgc gcctgcctag
     2941 gccgcgtggc cggggagccg gcggcggatg gcaccgttct ctgcccctgc tgccaggccc
     3001 ccacgcggcc gcaggcactc agcaccaacc tgcagctggc gcgcctggtg gaggggctgg
     3061 cccaggtgcc gcagggccac tgcgaggagc acctggaccc gctgagcatc tactgcgagc
     3121 aggaccgcgc gctggtgtgc ggagtgtgcg cctcactcgg ctcgcaccgc ggtcatcgcc
     3181 tcctgcctgc cgccgaggcc cacgcacgcc tcaagacaca gctgccacag cagaaactgc
     3241 agctgcagga ggcatgcatg cgcaaggaga agagtgtggc tgtgctggag catcagctgg
     3301 tggaggtgga ggagacagtg cgtcagttcc ggggggccgt gggggagcag ctgggcaaga
     3361 tgcgggtgtt cctggctgca ctggagggct ccttggaccg cgaggcagag cgtgtacggg
     3421 gtgaggcagg ggtcgccttg cgccgggagc tggggagcct gaactcttac ctggagcagc
     3481 tgcggcagat ggagaaggtc ctggaggagg tggcggacaa gccgcagact gagttcctca
     3541 tgaaatactg cctggtgacc agcaggctgc agaagatcct ggcagagtct cccccacccg
     3601 cccgtctgga catccagctg ccaattatct cagatgactt caaattccag gtgtggagga
     3661 agatgttccg ggctctgatg ccagcgctgg aggagctgac ctttgacccg agctctgcgc
     3721 acccgagcct ggtggtgtct tcctctggcc gccgcgtgga gtgctcggag cagaaggcgc
     3781 cgccggccgg ggaggacccg cgccagttcg acaaggcggt ggcggtggtg gcgcaccagc
     3841 agctctccga gggcgagcac tactgggagg tggatgttgg cgacaagccg cgctgggcgc
     3901 tgggcgtgat cgcggccgag gccccccgcc gcgggcgcct gcacgcggtg ccctcgcagg
     3961 gcctgtggct gctggggctg cgcgagggca agatcctgga ggcacacgtg gaggccaagg
     4021 agccgcgcgc tctgcgcagc cccgagaggc ggcccacgcg cattggcctt tacctgagct
     4081 tcggcgacgg cgtcctctcc ttctacgatg ccagcgacgc cgacgcgctc gtgccgcttt
     4141 ttgccttcca cgagcgcctg cccaggcccg tgtacccctt cttcgacgtg tgctggcacg
     4201 acaagggcaa gaatgcccag ccgctgctgc tcgtgggtcc cgaaggcgcc gaggccgaat
     4261 tcgaacaaaa actaatatca gaggaagatt tggaacagaa actaatcagt gaagaagatt
     4321 tagagcaaaa gttaatttcc gaggaggact tatagtgcag tcgacggtac cgcgggcccg
     4381 ggatcccgcg actctagata attctaccgg gtaggggagg cgcttttccc aaggcagtct
     4441 ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct
     4501 cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc
     4561 gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg
     4621 tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc
     4681 gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc
     4741 gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc
     4801 aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag
     4861 cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca
     4921 agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     4981 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     5041 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     5101 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     5161 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     5221 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     5281 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     5341 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     5401 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     5461 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     5521 gtgcctgacc gcgtctggaa caatcaacct ctggattaca aaatttgtga aagattgact
     5581 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     5641 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     5701 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     5761 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     5821 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     5881 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     5941 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     6001 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     6061 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     6121 gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga gcaatcacaa
     6181 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
     6241 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
     6301 tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt cactcccaac
     6361 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc
     6421 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc
     6481 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt
     6541 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt
     6601 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga
     6661 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg
     6721 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt
     6781 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     6841 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     6901 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     6961 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     7021 tgaatatcag agagtgagag gccttgacat tgctagcgtt ttaccgtcga cctctagcta
     7081 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     7141 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     7201 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7261 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     7321 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7381 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     7441 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     7501 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     7561 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     7621 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     7681 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     7741 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     7801 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     7861 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     7921 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     7981 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     8041 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     8101 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     8161 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     8221 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     8281 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     8341 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     8401 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     8461 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     8521 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     8581 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     8641 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     8701 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     8761 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     8821 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     8881 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     8941 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     9001 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     9061 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     9121 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     9181 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     9241 agtgccacct gacgtcgacg gatcgggaga tcaacttgtt tattgcagct tataatggtt
     9301 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     9361 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc
     9421 aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc
     9481 tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt
     9541 gtatttgtta aatatgtact acaaacttag tagtttttaa agaaattgta tttgttaaat
     9601 atgtactaca aacttagtag t
//
