LOCUS       V018354                10959 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018354
VERSION     V018354
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2827..5715
                     /label="Thbs4(NM_011582)"
                     /note="Thbs4(NM_011582)"
                     /gene="Thbs4"
     promoter        5747..6246
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             6267..6866
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6880..7468
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7675..8308
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8437..8453)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8461..8477
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8485..8515)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8530..8551
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8839..9427)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9598..10458)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10459..10563)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    10611..10745
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 gccaccatgc cggccccacg cgcggccgcg gccgccttcc tcctgctgca cctggtcctg
     2881 cagccgtggc agcggacgag cgcccaggcc acaccccagg tctttgatct tctaccgtcc
     2941 tccagccaga ggctgaaccc cagcgccctg cagccagtcc tgacagatcc caccctgcac
     3001 gaagtctatc tcatctccac cttcaagctg cagagtaaaa gctcagccac catctttggc
     3061 ctctactcct caagtgacaa cagcaaatac tttgaattca ctgtgatggg acggctgaac
     3121 aaagccatcc tccgctacct gaagaatgat ggaaagatcc atctggtggt gttcaacaac
     3181 ttgcagctgg ctgatgggag gcgccacagg gttctcctga gactgagcaa tttgcagaga
     3241 ggagatggct ctgtagaact ctacctggac tgcgctcagg cggactcggt gcgcaacctc
     3301 cccagagcct tttctggcct cacccagaac ccagagtcca ttgaattgcg gacattccag
     3361 aggaagcccc aggacttctt ggaagagctg aagctggtgg tgcggggctc gctgttccag
     3421 gtggccagcc tccaagactg cttcctgcag cagagtgagc ctctggctgc cacaagcaca
     3481 ggagacttta atcgacagtt cttgggtcaa atgacacaac tgaaccagct ccttggagag
     3541 gtgaaagatc ttctgaggca gcaggtcaag gaaacatcgt ttttgcgaaa caccattgct
     3601 gaatgccaag cttgtggtcc tctcagcttt cagtccccaa ctccaaacac actggtgccc
     3661 atagctcctc cagcaccccc aactcgccca acacgccact gtgactccag cccttgtttc
     3721 cgaggtgtcc gatgtacgga caccagggat ggcttccagt gtggaccctg cccagatggt
     3781 tacacgggaa acgggatcac ctgctctgac gtggatgagt gcaaatacca tccctgctat
     3841 ccaggtgtgc gctgtgtgaa tttggctccg ggtttcagat gtgacgcctg tccagtaggt
     3901 ttcacagggc ccatggtgca gggtgtcggg atcaactttg ctaaaacaaa caagcaggtg
     3961 tgcactgatg ttgatgagtg tcagaatgga gcgtgtgttc tgaattctat ctgcattaac
     4021 actttgggat cttatcgctg tgggccttgc aaaccagggt acactggtga tcagacgagg
     4081 ggatgcaaga ctgaaaggag ctgccggaac ccagagcaga acccatgcag tgtgcatgcg
     4141 cagtgcattg aggagcggca aggggatgtg acgtgtgtgt gtggtgttgg ctgggctggt
     4201 gacggctatg tctgtggaaa ggatgtggac attgacagtt accctgatga agaactgcca
     4261 tgctctgcca ggaactgcaa gaaggataac tgcaagtatg tgccaaactc tggccaggaa
     4321 gatgcagaca gagatggcat cggagacgcg tgtgatgagg atgcagatgg agatgggatc
     4381 ttgaatgaac aggacaactg tgtcctgact cacaatatag accaaaggaa cagtgataaa
     4441 gatatctttg gggatgcctg tgacaactgc cggatggtcc tgaataatga ccagaaggat
     4501 actgacgggg atgggagagg agacgcctgt gatgacgaca tggatggaga tggaataaaa
     4561 aacattttgg acaactgtcc cagagttccc aaccgtgacc agcaggatcg ggatggtgat
     4621 gatgtcggag atgcttgtga cagctgtcct gatgtcagta accctaacca gtcggatgtg
     4681 gataacgatc tggttgggga ctcctgtgac accaaccaag acagtgacgg ggatggccac
     4741 caggacagca cagacaactg ccccacagtc attaacagct cccagctgga cactgacaaa
     4801 gatgggatcg gcgatgaatg tgatgacgat gacgataacg atggcatccc agacctggtg
     4861 cctcctggcc cagacaactg caggctcgtc cccaacccag cccaggaaga tagcaacaat
     4921 gacggggtag gagatatctg tgaagctgac ttcgaccagg accaggtcat cgatcacatt
     4981 gatgtgtgcc ctgagaatgc ggagatcacc ctgactgact tcagggccta ccagacagtg
     5041 gtcctggacc ctgaagggga tgctcagatc gaccccaact gggtggtcct gaaccagggc
     5101 atggaaattg tgcagaccat gaacagtgac cctggcctag ctgttgggta cacggcattt
     5161 aacggagttg actttgaagg gaccttccac gtaaacaccc agacagatga tgactatgct
     5221 ggctttattt ttggttacca agacagctcc agcttctatg tagtcatgtg gaagcaaacg
     5281 gagcagacat actggcaggc tacaccgttc cgtgcagtag cagaacctgg tattcagctc
     5341 aaggctgtga agtctaaaac tggcccaggg gaacatctcc gaaactccct gtggcacaca
     5401 ggggacacca gcgaccaggt caggctgctg tggaaggact ccaggaatgt gggctggaag
     5461 gacaaggtct cctaccgctg gttcctgcag cataggcccc aggtgggcta catcagggtg
     5521 cgcttttatg aaggctcgga gttggtggct gactctgggg tcaccataga caccaccatg
     5581 cgtggaggcc gacttggtgt gttctgcttc tctcaagaaa acatcatctg gtccaacctc
     5641 aagtaccgct gcaacgacac cataccagag gacttccagg agttccagac ccagagtttc
     5701 gaccgcttgg ataattgagg atcccgcgac tctagataat tctaccgggt aggggaggcg
     5761 cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac
     5821 acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg
     5881 ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc
     5941 gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc
     6001 gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa
     6061 tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc
     6121 gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca
     6181 ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc
     6241 ctttcgacct gcagcccaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca
     6301 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     6361 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     6421 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     6481 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     6541 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     6601 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     6661 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     6721 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     6781 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     6841 gcatgacccg caagcccggt gcctgaccgc gtctggaaca atcaacctct ggattacaaa
     6901 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     6961 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     7021 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     7081 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     7141 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     7201 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     7261 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     7321 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     7381 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     7441 cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga gacctagaaa
     7501 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag
     7561 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa
     7621 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg ggactggaag
     7681 ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag
     7741 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
     7801 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
     7861 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     7921 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
     7981 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
     8041 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
     8101 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     8161 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     8221 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     8281 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     8341 ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg ctagcgtttt
     8401 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     8461 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     8521 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     8581 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     8641 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     8701 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8761 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8821 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8881 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8941 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     9001 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     9061 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     9121 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     9181 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9241 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     9301 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9361 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     9421 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     9481 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9541 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9601 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     9661 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     9721 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     9781 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     9841 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     9901 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     9961 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    10021 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    10081 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    10141 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    10201 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    10261 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    10321 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    10381 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    10441 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    10501 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    10561 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc aacttgttta
    10621 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
    10681 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
    10741 ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta
    10801 ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat
    10861 tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta gtttttaaag
    10921 aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
