LOCUS       V019466                10353 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V019466
VERSION     V019466
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2827..4476
                     /EC_number=""
                     /codon_start=1
                     /experiment=""
                     /gene="luc"
                     /product="firefly luciferase"
                     /protein_id=""
                     /transl_table=1
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
                     /label="luciferase"
     CDS             4498..5109
                     /label="Tnfaip8l3(NM_001033535)"
                     /note="Tnfaip8l3(NM_001033535)"
                     /gene="Tnfaip8l3"
     promoter        5141..5640
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             5661..6260
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6274..6862
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7069..7702
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(7831..7847)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7855..7871
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7879..7909)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7924..7945
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8233..8821)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8992..9852)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9853..9957)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    10005..10139
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 gccaccatgg aagacgccaa aaacataaag aaaggcccgg cgccattcta tcctcttgag
     2881 gatggaaccg ctggagagca actgcataag gctatgaaga gatacgccct ggttcctgga
     2941 acaattgctt ttacagatgc acatatcgag gtgaacatca cgtacgcgga atacttcgaa
     3001 atgtccgttc ggttggcaga agctatgaaa cgatatgggc tgaatacaaa tcacagaatc
     3061 gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc gttatttatc
     3121 ggagttgcag ttgcgcccgc gaacgacatt tataatgaac gtgaattgct caacagtatg
     3181 aacatttcgc agcctaccgt agtgtttgtt tccaaaaagg ggttgcaaaa aattttgaac
     3241 gtgcaaaaaa aattaccaat aatccagaaa attattatca tggattctaa aacggattac
     3301 cagggatttc agtcgatgta cacgttcgtc acatctcatc tacctcccgg ttttaatgaa
     3361 tacgattttg taccagagtc ctttgatcgt gacaaaacaa ttgcactgat aatgaactcc
     3421 tctggatcta ctgggttacc taagggtgtg gcccttccgc atagaactgc ctgcgtcaga
     3481 ttctcgcatg ccagagatcc tatttttggc aatcaaatca ttccggatac tgcgatttta
     3541 agtgttgttc cattccatca cggttttgga atgtttacta cactcggata tttgatatgt
     3601 ggatttcgag tcgtcttaat gtatagattt gaagaagagc tgtttttacg atcccttcag
     3661 gattacaaaa ttcaaagtgc gttgctagta ccaaccctat tttcattctt cgccaaaagc
     3721 actctgattg acaaatacga tttatctaat ttacacgaaa ttgcttctgg gggcgcacct
     3781 ctttcgaaag aagtcgggga agcggttgca aaacgcttcc atcttccagg gatacgacaa
     3841 ggatatgggc tcactgagac tacatcagct attctgatta cacccgaggg ggatgataaa
     3901 ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga aggttgtgga tctggatacc
     3961 gggaaaacgc tgggcgttaa tcagagaggc gaattatgtg tcagaggacc tatgattatg
     4021 tccggttatg taaacaatcc ggaagcgacc aacgccttga ttgacaagga tggatggcta
     4081 cattctggag acatagctta ctgggacgaa gacgaacact tcttcatagt tgaccgcttg
     4141 aagtctttaa ttaaatacaa aggataccag gtggcccccg ctgaattgga gtcgatattg
     4201 ttacaacacc ccaacatctt cgacgcgggc gtggcaggtc ttcccgacga tgacgccggt
     4261 gaacttcccg ccgccgttgt tgttttggag cacggaaaga cgatgacgga aaaagagatc
     4321 gtggattacg tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg agttgtgttt
     4381 gtggacgaag taccgaaagg tcttaccgga aaactcgacg caagaaaaat cagagagatc
     4441 ctcataaagg ccaagaaggg cggaaagtcc aaattggaat tcggaggagg gggaagcatg
     4501 gattcagatt ctggagagca gagcgagggc gagcccggga ctgctgcagg tcctcatgtg
     4561 tttagttcca agaatctcgc ccttcaagcc cagaagaaga tcctgagcaa gatagccagc
     4621 aaaactgtgg ccaacatgct gattgatgac accagcagtg agatctttga tgagctgtac
     4681 aaagtcacag aaatacacac ccacaacaag aaggaggccc acaagatcat gaaggatgct
     4741 atcaaggtgg caatcaaaat tggcattctc taccggaaca aacagttcag tcaagaggag
     4801 gttataattg tggagaaact ccggaagaaa ctgaaccaga ctgcgatgac catggtcagc
     4861 ttctacgaag tagagtatac ctttgatacg aatgtgctct ctaagcttct gcatgagtgc
     4921 aaggacctgg tacatgaact ggtacagcga cacttgacac ccagaaccca tggacgcata
     4981 aaccatgtct tcaaccactt tgctgatgtg gaattccttt ccactctcta tggtccgcat
     5041 ggaaactgca ggcccaatct caagaggatt tgcgaaggaa tcaataaatt gttagacgac
     5101 aagatcctct gaggatcccg cgactctaga taattctacc gggtagggga ggcgcttttc
     5161 ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt
     5221 ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt
     5281 ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg
     5341 cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag
     5401 atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag
     5461 ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc
     5521 aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc
     5581 acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg
     5641 acctgcagcc caagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     5701 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     5761 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     5821 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     5881 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     5941 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     6001 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     6061 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     6121 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     6181 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     6241 cccgcaagcc cggtgcctga ccgcgtctgg aacaatcaac ctctggatta caaaatttgt
     6301 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     6361 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     6421 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     6481 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     6541 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     6601 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     6661 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     6721 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     6781 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     6841 tccctttggg ccgcctcccc gcctggaatt aattctgcag tcgagaccta gaaaaacatg
     6901 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac
     6961 aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt
     7021 acaaggcagc tgtagatctt agccactttt taaaagaaaa gaggggactg gaagggctaa
     7081 ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
     7141 tccctgatta gcagaactac acaccagggc caggggtcag atatccactg acctttggat
     7201 ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga
     7261 acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt
     7321 tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg
     7381 agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc
     7441 cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag
     7501 cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     7561 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     7621 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     7681 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     7741 cttgcaaaga aatgaatatc agagagtgag aggccttgac attgctagcg ttttaccgtc
     7801 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     7861 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     7921 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7981 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     8041 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     8101 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     8161 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8221 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8281 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8341 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8401 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8461 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8521 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8581 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8641 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     8701 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8761 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8821 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8881 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8941 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     9001 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     9061 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9121 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9181 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9241 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9301 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9361 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9421 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9481 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9541 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9601 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9661 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9721 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9781 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9841 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9901 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9961 atttccccga aaagtgccac ctgacgtcga cggatcggga gatcaacttg tttattgcag
    10021 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
    10081 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca
    10141 actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca
    10201 ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
    10261 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttttt aaagaaattg
    10321 tatttgttaa atatgtacta caaacttagt agt
//
