LOCUS       V019952                11472 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V019952
VERSION     V019952
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2827..6201
                     /label="USP25(NM_001283041)"
                     /note="USP25(NM_001283041)"
                     /gene="USP25"
     CDS             6202..6228
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        6260..6759
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             6780..7379
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7393..7981
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8188..8821
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8950..8966)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8974..8990
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8998..9028)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9043..9064
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9352..9940)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10111..10971)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10972..11076)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    11124..11258
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 gccaccatga ccgtggagca gaacgtgctg cagcagagcg cggcgcagaa gcaccagcag
     2881 acgtttttga atcaactgag agaaattacg gggattaatg acacccagat actacagcaa
     2941 gccttgaagg atagtaatgg aaacttggaa ttagcagtgg ctttccttac tgcgaagaat
     3001 gctaagaccc ctcagcagga ggagacaact tactaccaaa cagcacttcc tggcaatgat
     3061 agatacatca gtgtgggaag ccaagcagat acaaatgtga ttgatctcac tggagatgat
     3121 aaagatgatc ttcagagagc aattgccttg agtttggccg aatcaaacag ggcattcagg
     3181 gagactggaa taactgatga ggaacaagcc attagcagag ttcttgaagc cagcatagca
     3241 gagaataaag catgtttgaa gaggacacct acagaagttt ggagggattc tcgaaaccct
     3301 tatgatagaa aaagacagga caaagctccc gttgggctaa agaatgttgg caatacttgt
     3361 tggtttagtg ctgttattca gtcattattt aatcttttgg aatttagaag attagttctg
     3421 aattacaagc ctccatcaaa tgctcaagat ttaccccgaa accaaaagga acatcggaat
     3481 ttgcctttta tgcgtgagct gaggtatcta tttgcacttc ttgttggtac caaaaggaag
     3541 tatgttgatc catcaagagc agttgaaatt cttaaggatg ctttcaaatc aaatgactca
     3601 cagcagcaag atgtgagtga gtttacacac aaattattag attggttaga agatgccttc
     3661 caaatgaaag ctgaagagga gacggatgaa gagaagccaa agaaccccat ggtagagttg
     3721 ttctatggca gattcctggc tgtgggagta cttgaaggta aaaaatttga aaacactgaa
     3781 atgtttggtc agtacccact tcaggtcaat gggttcaaag atctgcatga gtgcctagaa
     3841 gctgcaatga ttgaaggaga aattgagtct ttacattcag agaattcagg aaaatcaggc
     3901 caagagcatt ggtttactga attaccacct gtgttaacat ttgaattgtc aagatttgaa
     3961 tttaatcagg cattgggaag accagaaaaa attcacaaca aattagaatt tccccaagtt
     4021 ttatatttgg acagatacat gcacagaaac agagaaataa caagaattaa gagggaagag
     4081 atcaagagac tgaaagatta cctcacggta ttacaacaaa ggctagaaag atatttaagc
     4141 tatggttccg gtcccaaacg attccccttg gtagatgttc ttcagtatgc attggaattt
     4201 gcctcaagta aacctgtttg cacttctcct gttgacgata ttgacgctag ttccccacct
     4261 agtggttcca taccatcaca gacattacca agcacaacag aacaacaggg agccctatct
     4321 tcagaactgc caagcacagc accttcatca gttgctgcca tttcatcgag atcagtaata
     4381 cacaaaccat ttactcagtc ccggatacct ccagatttgc ccatgcatcc ggcaccaagg
     4441 cacataacgg aggaagaact ttctgtgctg gaaagttgtt tacatcgctg gaggacagaa
     4501 atagaaaatg acaccagaga tttgcaggaa agcatatcca gaatccatcg aacaattgaa
     4561 ttaatgtact ctgacaaatc tatgatacaa gttccttatc gattacatgc cgttttagtt
     4621 cacgaaggcc aagctaatgc tgggcactac tgggcatata tttttgatca tcgtgaaagc
     4681 agatggatga agtacaatga tattgctgtg acaaaatcat catgggaaga gctagtgagg
     4741 gactcttttg gtggttatag aaatgccagt gcatactgtt taatgtacat aaatgataag
     4801 gcacagttcc taatacaaga ggagtttaat aaagaaactg ggcagcccct tgttggtata
     4861 gaaacattac caccggattt gagagatttt gttgaggaag acaaccaacg atttgaaaaa
     4921 gaactagaag aatgggatgc acaacttgcc cagaaagctt tgcaggaaaa gcttttagcg
     4981 tctcagaaat tgagagagtc agagacttct gtgacaacag cacaagcagc aggagaccca
     5041 gaatatctag agcagccatc aagaagtgat ttctcaaagc acttgaaaga agaaactatt
     5101 caaataatta ccaaggcatc acatgagcat gaagataaaa gtcctgaaac agttttgcag
     5161 tcgaaacctg aaaatactac aagccaacca ctttctaatc agcgagttgt agaggtggcg
     5221 atccctcatg tagggaaatt tatgattgaa tcaaaggagg gggggtatga tgacgagatc
     5281 atgatgacac cgaacatgca aggtattatc atggcgatag gtaaatccag gagtgtatat
     5341 gacaggtgtg gccctgaagc agggttcttt aaggcaatta agttggaata tgcaaggttg
     5401 gttaagttgg cccaagaaga caccccacca gaaaccgatt atcgtttaca tcatgtagtg
     5461 gtctacttta tccagaacca ggcaccaaag aaaattattg agaaaacatt actagaacaa
     5521 tttggagata gaaatttgag ttttgatgaa aggtgtcaca acataatgaa agttgctcaa
     5581 gccaaactgg aaatgataaa acctgaagaa gtaaacttgg aggaatatga ggagtggcat
     5641 caggattata ggaaattcag ggaaacaact atgtatctca taattgggct agaaaatttt
     5701 caaagagaaa gttatataga ttccttgctg ttcctcatct gtgcttatca gaataacaaa
     5761 gaactcttgt ctaaaggctt atacagagga catgatgaag aattgatatc acattataga
     5821 agagaatgtt tgctaaaatt aaatgagcaa gccgcagaac tcttcgaatc tggagaggat
     5881 cgagaagtaa acaatggttt gattatcatg aatgagttta ttgtcccatt tttgccatta
     5941 ttactggtgg atgaaatgga agaaaaggat atactagctg tagaagatat gagaaatcga
     6001 tggtgttcct accttggtca agaaatggaa ccacacctcc aagaaaagct gacagatttt
     6061 ttgccaaaac tgcttgattg ttctatggag attaaaagtt tccatgagcc accgaagtta
     6121 ccttcatatt ccacgcatga actctgtgag cgatttgccc gaatcatgtt gtccctcagt
     6181 cgaactcctg ctgatggaag atacccatac gacgtaccag attacgctta aggatcccgc
     6241 gactctagat aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc
     6301 gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat
     6361 tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct
     6421 tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg
     6481 tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa
     6541 tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg
     6601 gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg
     6661 gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct
     6721 gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttacca
     6781 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
     6841 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
     6901 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     6961 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     7021 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     7081 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     7141 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     7201 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     7261 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     7321 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac
     7381 cgcgtctgga acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     7441 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     7501 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     7561 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     7621 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     7681 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     7741 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     7801 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     7861 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     7921 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     7981 cctggaatta attctgcagt cgagacctag aaaaacatgg agcaatcaca agtagcaata
     8041 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
     8101 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta
     8161 gccacttttt aaaagaaaag aggggactgg aagggctaat tcactcccaa cgaagacaag
     8221 atatccttga tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca
     8281 caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag
     8341 ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg
     8401 tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc
     8461 gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat
     8521 atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg
     8581 ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact
     8641 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     8701 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     8761 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     8821 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     8881 gagagtgaga ggccttgaca ttgctagcgt tttaccgtcg acctctagct agagcttggc
     8941 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     9001 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     9061 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     9121 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     9181 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     9241 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     9301 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     9361 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     9421 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     9481 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     9541 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     9601 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     9661 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     9721 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     9781 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     9841 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     9901 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     9961 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
    10021 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
    10081 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
    10141 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
    10201 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
    10261 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
    10321 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
    10381 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
    10441 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
    10501 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
    10561 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
    10621 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
    10681 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
    10741 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    10801 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    10861 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    10921 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    10981 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    11041 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    11101 tgacgtcgac ggatcgggag atcaacttgt ttattgcagc ttataatggt tacaaataaa
    11161 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    11221 tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc
    11281 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc
    11341 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
    11401 aaatatgtac tacaaactta gtagttttta aagaaattgt atttgttaaa tatgtactac
    11461 aaacttagta gt
//
