LOCUS       V026124                10579 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026124
VERSION     V026124
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2843..4639
                     /label="ZC3H12A(NM_025079)"
                     /note="ZC3H12A(NM_025079)"
                     /gene="ZC3H12A"
     CDS             4667..5386
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="Aequorea coerulescens GFP"
                     /transl_table=1
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK*"
                     /label="AcGFP1"
     promoter        5414..5913
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             5934..6533
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6547..7135
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7342..7975
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8104..8120)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8128..8144
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8152..8182)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8197..8218
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8506..9094)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9265..10125)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10126..10230)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    10278..10412
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcaagcttc gaattcgcca ccatgagtgg cccctgtgga gagaagcctg tcctggaagc
     2881 cagccccacc atgagtctgt gggaatttga ggacagccac agccgtcagg gcaccccaag
     2941 gccgggtcaa gagctggccg ctgaggaggc ctcggccctg gaactgcaga tgaaggtgga
     3001 cttcttccgg aagctgggct attcatccac ggagatccac agcgtcctgc agaagctggg
     3061 cgtccaggca gacaccaaca cggtgctggg tgagctggtg aaacacggga cagccaccga
     3121 gcgggagcgc cagacctcac cggacccctg ccctcagctc cctctagtcc cgcggggtgg
     3181 tggcacccct aaggctccca acctggagcc tccactccca gaagaggaaa aggagggcag
     3241 cgacctgaga ccagtggtca tcgatgggag caacgtggcc atgagccatg ggaacaagga
     3301 ggtcttctcc tgccggggca tcctgctggc agtgaactgg tttctggagc ggggccacac
     3361 agacatcaca gtgtttgtgc catcctggag gaaggagcag cctcggcccg acgtgcccat
     3421 cacagaccag cacatcctgc gggaactgga gaagaagaag atcctggtgt tcacaccatc
     3481 acgacgcgtg ggtggcaagc gggtggtgtg ctatgacgac agattcattg tgaagctggc
     3541 ctacgagtct gacgggatcg tggtttccaa cgacacatac cgtgacctcc aaggcgagcg
     3601 gcaggagtgg aagcgcttca tcgaggagcg gctgctcatg tactccttcg tcaatgacaa
     3661 gtttatgccc cctgatgacc cactgggccg gcacgggccc agcctggaca acttcctgcg
     3721 taagaagcca ctcactttgg agcacaggaa gcagccgtgt ccctatggaa ggaaatgcac
     3781 ctatgggatc aagtgccgat tcttccaccc agagcggcca agctgccccc agcgctctgt
     3841 ggcagatgag ctccgtgcca atgctctcct ctcacccccc agagccccaa gcaaggacaa
     3901 aaatggccgg cggccttcac cttcatccca gtccagctct ctgctaacag agagtgagca
     3961 gtgcagcctg gatgggaaga agctgggggc ccaggcatcc ccagggtccc gccaagaggg
     4021 tctaacacag acctatgccc catcaggcag gagcctcgca cctagcgggg gcagtggcag
     4081 cagctttggg cccacagact ggctcccaca gacgctggac tcactcccgt acgtctccca
     4141 ggattgcctg gactcgggca ttggctccct ggagagccag atgtcggaac tttggggggt
     4201 tcgaggagga ggccctggtg agccgggccc accccgagcc ccttacacgg gctacagtcc
     4261 ctatggatct gagctcccag ccaccgcagc cttctctgcc tttggccggg ccatgggtgc
     4321 tggccacttc agtgtccctg ccgactaccc acccgcgccc cctgcctttc cacctcgaga
     4381 gtactggtct gaaccatacc cactgccccc acccacatca gtccttcagg agcccccagt
     4441 gcagagccca ggggctggca ggagcccgtg gggcagggca ggcagcctgg ccaaggagca
     4501 ggccagcgtg tatactaagc tgtgtggtgt gtttcccccg cacctggtgg aggctgtgat
     4561 ggggcgcttc ccacagctcc tggaccccca gcagctggct gccgagatcc tctcctacaa
     4621 gtcccagcac cccagtgagg gtggaggtgg atcggatcca ccggtcatgg tgagcaaggg
     4681 cgccgagctg ttcaccggca tcgtgcccat cctgatcgag ctgaatggcg atgtgaatgg
     4741 ccacaagttc agcgtgagcg gcgagggcga gggcgatgcc acctacggca agctgaccct
     4801 gaagttcatc tgcaccaccg gcaagctgcc tgtgccctgg cccaccctgg tgaccaccct
     4861 gagctacggc gtgcagtgct tctcacgcta ccccgatcac atgaagcagc acgacttctt
     4921 caagagcgcc atgcctgagg gctacatcca ggagcgcacc atcttcttcg aggatgacgg
     4981 caactacaag tcgcgcgccg aggtgaagtt cgagggcgat accctggtga atcgcatcga
     5041 gctgaccggc accgatttca aggaggatgg caacatcctg ggcaataaga tggagtacaa
     5101 ctacaacgcc cacaatgtgt acatcatgac cgacaaggcc aagaatggca tcaaggtgaa
     5161 cttcaagatc cgccacaaca tcgaggatgg cagcgtgcag ctggccgacc actaccagca
     5221 gaataccccc atcggcgatg gccctgtgct gctgcccgat aaccactacc tgtccaccca
     5281 gagcgccctg tccaaggacc ccaacgagaa gcgcgatcac atgatctact tcggcttcgt
     5341 gaccgccgcc gccatcaccc acggcatgga tgagctgtac aagtgagcgg ccgcgactct
     5401 agataattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta
     5461 gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca
     5521 tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact
     5581 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa
     5641 atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag
     5701 cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag
     5761 aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc
     5821 gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg
     5881 ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt accatgaccg
     5941 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
     6001 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
     6061 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     6121 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     6181 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     6241 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     6301 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     6361 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     6421 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     6481 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgaccgcgtc
     6541 tggaacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     6601 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     6661 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     6721 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     6781 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     6841 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     6901 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     6961 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     7021 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     7081 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga
     7141 attaattctg cagtcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag
     7201 ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag
     7261 tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     7321 ttttaaaaga aaagagggga ctggaagggc taattcactc ccaacgaaga caagatatcc
     7381 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag
     7441 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     7501 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
     7561 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag
     7621 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag
     7681 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg
     7741 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct
     7801 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     7861 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     7921 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     7981 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     8041 gagaggcctt gacattgcta gcgttttacc gtcgacctct agctagagct tggcgtaatc
     8101 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     8161 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     8221 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     8281 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     8341 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     8401 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     8461 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     8521 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     8581 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     8641 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     8701 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     8761 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     8821 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     8881 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     8941 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     9001 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     9061 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     9121 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     9181 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     9241 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     9301 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     9361 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     9421 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     9481 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     9541 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     9601 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     9661 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     9721 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9781 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9841 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     9901 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9961 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    10021 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    10081 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    10141 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    10201 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    10261 cgacggatcg ggagatcaac ttgtttattg cagcttataa tggttacaaa taaagcaata
    10321 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
    10381 aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc
    10441 atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac
    10501 tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat
    10561 gtactacaaa cttagtagt
//
