LOCUS       V038908                10722 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038908
VERSION     V038908
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        105..408
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        409..612
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     protein_bind    635..659
                     /bound_moiety="BP Clonase™"
                     /gene="mutant version of&nbsp;attB"
                     /note="recombination site for the Gateway® BP reaction"
                     /label="attB1"
     CDS             836..1582
                     /codon_start=1
                     /note="more active than the original APEX due to the A134P
                     mutation"
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell
                     proteomics (Lam et al., 2015)"
                     /transl_table=1
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
                     /label="APEX2"
     CDS             1583..3544
                     /label="WDR70(NM_018034)"
                     /note="WDR70(NM_018034)"
                     /gene="WDR70"
     CDS             3545..3610
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     CDS             3611..3652
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /transl_table=1
                     /translation="GKPIPNPLLGLDST"
                     /label="V5 tag"
     protein_bind    complement(3660..3684)
                     /bound_moiety="BP Clonase™"
                     /gene="mutant version of&nbsp;attB"
                     /note="recombination site for the Gateway® BP reaction"
                     /label="attB2"
     CDS             3686..3727
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /transl_table=1
                     /translation="GKPIPNPLLGLDST"
                     /label="V5 tag"
     misc_feature    3769..4357
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             4429..4662
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    4740..4861
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      4901..5036
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     promoter        complement(5057..5075)
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     primer_bind     complement(5085..5101)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     rep_origin      5243..5698
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5724..5828
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             5829..6689
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      6860..7448
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     protein_bind    7736..7757
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        7772..7802
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7810..7826
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     primer_bind     7834..7850
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     promoter        7871..7889
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /label="T3 promoter"
     promoter        7917..8143
                     /note="Rous sarcoma virus enhancer/promoter"
                     /label="RSV promoter"
     LTR             8144..8324
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    8371..8496
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    8989..9222
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             9407..9451
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     promoter        9626..10126
                     /note="human phosphoglycerate kinase 1 promoter"
                     /label="hPGK promoter"
     CDS             10138..10533
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /note="confers resistance to blasticidin"
                     /product="blasticidin S deaminase"
                     /transl_table=1
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
                     /label="BSD"
     misc_feature    10593..10710
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
ORIGIN
        1 agggacagca gagatccact ttggcgccgg ctcgaggggg cccggggtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctctctggct aactgtcggg atcaacaagt ttgtacaaaa aagcaggctt
      661 cgaaggagat agaaccatgg accctgtggt cgtgctgggg ctgtgcctga gctgtctgct
      721 gctgctgagc ctgtggaagc agtcctacgg aggcggggga tcaggaagcg gcggccgcgg
      781 atcaggaagc ggatcaggaa gcggatcagg aagcggatca ggaagcggat caggaggaaa
      841 gtcttaccca actgtgagtg ctgattacca ggacgccgtt gagaaggcga agaagaagct
      901 cagaggcttc atcgctgaga agagatgcgc tcctctaatg ctccgtttgg cattccactc
      961 tgctggaacc tttgacaagg gcacgaagac cggtggaccc ttcggaacca tcaagcaccc
     1021 tgccgaactg gctcacagcg ctaacaacgg tcttgacatc gctgttaggc ttttggagcc
     1081 actcaaggcg gagttcccta ttttgagcta cgccgatttc taccagttgg ctggcgttgt
     1141 tgccgttgag gtcacgggtg gacctaaggt tccattccac cctggaagag aggacaagcc
     1201 tgagccacca ccagagggtc gcttgcccga tcccactaag ggttctgacc atttgagaga
     1261 tgtgtttggc aaagctatgg ggcttactga ccaagatatc gttgctctat ctgggggtca
     1321 cactattgga gctgcacaca aggagcgttc tggatttgag ggtccctgga cctctaatcc
     1381 tcttattttc gacaactcat acttcacgga gttgttgagt ggtgagaagg aaggtctcct
     1441 tcagctacct tctgacaagg ctcttttgtc tgaccctgta ttccgccctc tcgttgacaa
     1501 atatgcagcg gacgaagatg ccttctttgc tgattacgct gaggctcacc aaaagctttc
     1561 cgagcttggg tttgctgatg ccatggagcg ctctgggccc agcgaagtga caggctcaga
     1621 cgcgtcggga ccggacccgc agcttgcggt caccatgggc ttcacggggt tcggtaaaaa
     1681 agctcgcaca tttgacttgg aagcaatgtt tgaacaaact cgaaggacag ctgtggaaag
     1741 aagtcgcaaa acactggaag caagagaaaa agaggaagaa atgaacagag agaaagaatt
     1801 aagaagacaa aatgaagata ttgagccaac atcctcaaga tcaaatgtgg tcagagattg
     1861 ctccaaatca tcttccaggg atacgagcag cagtgaaagt gaacagagtt ctgactcttc
     1921 tgatgatgag ttaattggcc ctcctttacc ccctaaaatg gtaggaaaac cagttaattt
     1981 tatggaggaa gatatcctcg gtcctttacc tccacctctt aatgaagaag aagaagaagc
     2041 agaggaagaa gaagaggaag aggaggaaga ggaaaatcct gttcacaaga ttcctgactc
     2101 gcatgagata acgctgaagc atggcactaa aacagtgtct gctttgggtc tggatccctc
     2161 aggtgcccgt ttggtgacag gaggatatga ctatgatgtt aagttttggg attttgctgg
     2221 aatggatgct tcttttaagg catttcgatc ccttcagccc tgtgagtgcc atcagatcaa
     2281 gtcattacag tatagtaaca caggagacat gattcttgtt gtatctggaa gctctcaggc
     2341 caaggtgatt gacagagatg gttttgaagt aatggaatgt ataaaaggag accagtatat
     2401 tgtggacatg gccaacacca agggtcatac agcaatgctt catactggct catggcatcc
     2461 caaaataaag ggagaattta tgacttgctc aaatgatgcg actgtgagga cgtgggaagt
     2521 tgaaaatcca aagaagcaaa aaagtgtgtt taaaccacgg acgatgcaag gcaaaaaagt
     2581 cattcccact acgtgcacat atagtagaga tggaaacctc atagcagctg cctgccagaa
     2641 tggaagcata cagatctggg accgaaattt gactgttcat cctaagttcc actataaaca
     2701 ggctcatgac tcgggcacag acacttcttg cgtgactttt tcctatgatg gtaatgtcct
     2761 tgcctctcgt ggaggtgacg attcattaaa attatgggac atccgacaat ttaataaacc
     2821 acttttttca gcctcgggtc ttcccaccat gttcccaatg actgactgct gtttcagtcc
     2881 agatgataag ctcatagtca ctggtacatc tattcaaaga ggatgtggca gcggcaaact
     2941 tgttttcttt gagcgtagga ctttccaaag ggtgtatgaa atagacatca cagatgcgag
     3001 tgttgttcgc tgcctgtggc atccaaagct gaaccagatc atggttggaa ctggaaatgg
     3061 attggctaaa gtctattacg accccaacaa gagtcagagg ggagcaaaat tatgtgtggt
     3121 taaaacccag cggaaggcaa aacaagctga gactctaact caggactaca tcatcacccc
     3181 tcatgccttg cctatgttcc gtgagccccg ccaacggagt acaaggaaac agctggagaa
     3241 ggacagactg gatcccctga agtcgcataa acctgaacct cctgtagcag gcccaggtcg
     3301 tggtggccga gttggaaccc acgggggcac tctctcttcc tatattgtga agaacattgc
     3361 tttggacaag accgatgaca gtaatcctcg ggaagccatt ttgcgtcatg ccaaagcagc
     3421 agaagacagc ccatattggg tttctccagc atattccaag actcagccca aaaccatgtt
     3481 tgcccaagtt gaatctgatg atgaggaagc aaagaatgag ccagaatgga aaaaacgtaa
     3541 aattgactac aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga
     3601 cgatgacaag ggcaagccca tccccaaccc cctgctgggc ctggacagca cctgataaga
     3661 cccagctttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc tcggtctcga
     3721 ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg
     3781 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     3841 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     3901 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     3961 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     4021 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     4081 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     4141 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     4201 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     4261 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     4321 cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt
     4381 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     4441 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     4501 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     4561 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     4621 gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat
     4681 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     4741 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     4801 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     4861 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     4921 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     4981 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     5041 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     5101 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     5161 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     5221 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     5281 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     5341 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     5401 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     5461 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     5521 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     5581 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     5641 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     5701 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     5761 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     5821 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     5881 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     5941 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     6001 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     6061 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     6121 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     6181 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     6241 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     6301 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     6361 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     6421 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     6481 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     6541 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     6601 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     6661 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     6721 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     6781 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     6841 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     6901 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     6961 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     7021 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     7081 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     7141 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7201 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7261 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7321 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7381 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7441 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     7501 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     7561 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     7621 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     7681 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     7741 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     7801 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     7861 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta
     7921 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt
     7981 acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc
     8041 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca
     8101 ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga
     8161 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     8221 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     8281 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     8341 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     8401 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     8461 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     8521 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     8581 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     8641 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     8701 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     8761 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     8821 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     8881 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     8941 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     9001 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     9061 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     9121 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     9181 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     9241 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     9301 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     9361 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     9421 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     9481 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     9541 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     9601 ccacctccca accccgaggg gacccttgcg ccttttccaa ggcagccctg ggtttgcgca
     9661 gggacgcggc tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg
     9721 cacattcttc acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc
     9781 cccggcgacg cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg
     9841 ccggacgtga caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca
     9901 gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcagg
     9961 gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt
    10021 gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc
    10081 ggcagtcggc tccctcgttg accgaatcac cgacctctct ccccaggggg taccaccatg
    10141 gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc tacaatcaac
    10201 agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag cgacggccgc
    10261 atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga actcgtggtg
    10321 ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc gatcggaaat
    10381 gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct cgatctgcat
    10441 cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt tgggattcgt
    10501 gaattgctgc cctctggtta tgtgtgggag ggcctgcagc tgcagtagta agaattctag
    10561 atcttgagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
    10621 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
    10681 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt ac
//
