LOCUS pLenti6_TR. 8354 bp DNA circular SYN 01-JAN-1980 DEFINITION Lentiviral vector for high-level constitutive expression of the Tet repressor. ACCESSION . VERSION . KEYWORDS pLenti6 TR. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8354) AUTHORS Invitrogen (Life Technologies) TITLE Direct Submission REFERENCE 2 (bases 1 to 8354) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..8354 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 3..229 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 230..410 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 457..582 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1075..1308 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1493..1537 /label=gp41 peptide /note="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" CDS 1686..1727 /note="Protein Tat from Human immunodeficiency virus type 1 group M subtype B (isolate WMJ22). Accession#: P12509" enhancer 1816..2119 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 2120..2323 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" intron 2552..3124 /label=beta-globin intron /note="intron from rabbit beta-globin gene" promoter 3179..3197 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 3208..3828 /label=TetR /note="tetracycline repressor TetR" CDS 3870..3899 /label=Myc /note="Myc (human c-Myc proto-oncogene) epitope tag" promoter 3947..4276 /label=SV40 promoter /note="SV40 enhancer and early promoter" promoter 4324..4371 /label=EM7 promoter /note="synthetic bacterial promoter" CDS 4390..4785 /label=BSD /note="blasticidin S deaminase" LTR 4875..5108 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 5180..5314 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin 5341..5476 /label=SV40 ori /note="SV40 origin of replication" promoter complement(5497..5515) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(5525..5541) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" rep_origin 5683..6138 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 6164..6268 /label=AmpR promoter CDS 6269..7126 /label=AmpR /note="beta-lactamase" rep_origin 7300..7888 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 8176..8197 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 8212..8242 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8250..8266 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 8274..8290 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" promoter 8311..8329 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" ORIGIN 1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca 61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga 121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt 181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg 241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg 421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt 481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg 541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga 601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta 661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta 721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga 781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg 841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt 901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga 961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc 1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc 1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct 1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag 1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca 1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg 1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa 1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgata 1801 agcttgggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa 1861 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac 1921 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca 1981 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg 2041 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt 2101 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg 2161 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg 2221 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat 2281 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca 2341 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gactctagag 2401 gatccactag tccagtgtgg tggaattctg cagatagctt ggtacccggg gatcctctag 2461 ggcctctgag ctattccaga agtagtgaag aggctttttt ggaggcctag gcttttgcaa 2521 aaagctccgg atcgatcctg agaacttcag ggtgagtttg gggacccttg attgttcttt 2581 ctttttcgct attgtaaaat tcatgttata tggagggggc aaagttttca gggtgttgtt 2641 tagaatggga agatgtccct tgtatcacca tggaccctca tgataatttt gtttctttca 2701 ctttctactc tgttgacaac cattgtctcc tcttattttc ttttcatttt ctgtaacttt 2761 ttcgttaaac tttagcttgc atttgtaacg aatttttaaa ttcacttttg tttatttgtc 2821 agattgtaag tactttctct aatcactttt ttttcaaggc aatcagggta tattatattg 2881 tacttcagca cagttttaga gaacaattgt tataattaaa tgataaggta gaatatttct 2941 gcatataaat tctggctggc gtggaaatat tcttattggt agaaacaact acatcctggt 3001 catcatcctg cctttctctt tatggttaca atgatataca ctgtttgaga tgaggataaa 3061 atactctgag tccaaaccgg gcccctctgc taaccatgtt catgccttct tctttttcct 3121 acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattgta 3181 atacgactca ctatagggcg aattgatatg tctagattag ataaaagtaa agtgattaac 3241 agcgcattag agctgcttaa tgaggtcgga atcgaaggtt taacaacccg taaactcgcc 3301 cagaagctag gtgtagagca gcctacattg tattggcatg taaaaaataa gcgggctttg 3361 ctcgacgcct tagccattga gatgttagat aggcaccata ctcacttttg ccctttagaa 3421 ggggaaagct ggcaagattt tttacgtaat aacgctaaaa gttttagatg tgctttacta 3481 agtcatcgcg atggagcaaa agtacattta ggtacacggc ctacagaaaa acagtatgaa 3541 actctcgaaa atcaattagc ctttttatgc caacaaggtt tttcactaga gaatgcatta 3601 tatgcactca gcgctgtggg gcattttact ttaggttgcg tattggaaga tcaagagcat 3661 caagtcgcta aagaagaaag ggaaacacct actactgata gtatgccgcc attattacga 3721 caagctatcg aattatttga tcaccaaggt gcagagccag ccttcttatt cggccttgaa 3781 ttgatcatat gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc gtacagcgga 3841 tcccgggaat tctagagggc ccgcggttcg aacaaaaact catctcagaa gaggatctga 3901 atatgcatac cggttagtaa tgagtttgga attaattctg tggaatgtgt gtcagttagg 3961 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 4021 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 4081 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac 4141 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga 4201 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg 4261 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca 4321 cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg 4381 aactaaacca tggccaagcc tttgtctcaa gaagaatcca ccctcattga aagagcaacg 4441 gctacaatca acagcatccc catctctgaa gactacagcg tcgccagcgc agctctctct 4501 agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg accttgtgca 4561 gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc 4621 gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg acaggtgctt 4681 ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca gccgacggca 4741 gttgggattc gtgaattgct gccctctggt tatgtgtggg agggctaagc acaattcgag 4801 ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa 4861 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc 4921 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 4981 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 5041 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 5101 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg 5161 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa 5221 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 5281 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc 5341 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 5401 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 5461 ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc 5521 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta 5581 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg 5641 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg 5701 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc 5761 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc 5821 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg 5881 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg 5941 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg 6001 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt 6061 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa 6121 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 6181 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 6241 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 6301 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 6361 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 6421 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 6481 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg 6541 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 6601 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 6661 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 6721 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 6781 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 6841 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 6901 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 6961 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 7021 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 7081 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 7141 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 7201 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 7261 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 7321 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 7381 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 7441 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 7501 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 7561 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 7621 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 7681 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 7741 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 7801 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 7861 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 7921 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 7981 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 8041 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc 8101 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag 8161 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt 8221 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa 8281 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct 8341 ggagctgcaa gctt //